This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Ahrabi A.F., Handa D., Codipilly C.N., Shah S., Williams J.E., McGuire M.A., Potak D., Aharon G.G., Schanler R.J.: Effects of extended freezer storage on the integrity of human milk. J Pediatr. 177, 140–143 (2016)AhrabiA.F.HandaD.CodipillyC.N.ShahS.WilliamsJ.E.McGuireM.A.PotakD.AharonG.G.SchanlerR.J.: Effects of extended freezer storage on the integrity of human milk. J Pediatr. 177, 140–143 (2016)Search in Google Scholar
Alghamdi S., Ashshi A., Kabrah A., Atwah B., Alkurbi M.O., Assas M.B.: The impact of gastrointestinal tract infection on acquiring dengue fever virus infection and haemorrhagic fever in Jeddah region, Saudi Arabia. Cell Mol. Biol. 68(9), 45–50 (2022)AlghamdiS.AshshiA.KabrahA.AtwahB.AlkurbiM.O.AssasM.B.: The impact of gastrointestinal tract infection on acquiring dengue fever virus infection and haemorrhagic fever in Jeddah region, Saudi Arabia. Cell Mol. Biol. 68(9), 45–50 (2022)Search in Google Scholar
Amar C.F., East C.L., Grant K.A., Gray J., Iturriza-Gomara M., Maclure E.A., McLauchlin J.: Detection of viral, bacterial, and parasitological RNA or DNA of nine intestinal pathogens in fecal samples archived as part of the English infectious intestinal disease study: assessment of the stability of target nucleic acid. Diagn. Mol. Pathol. 14(2), 90–96 (2005)AmarC.F.EastC.L.GrantK.A.GrayJ.Iturriza-GomaraM.MaclureE.A.McLauchlinJ.: Detection of viral, bacterial, and parasitological RNA or DNA of nine intestinal pathogens in fecal samples archived as part of the English infectious intestinal disease study: assessment of the stability of target nucleic acid. Diagn. Mol. Pathol. 14(2), 90–96 (2005)Search in Google Scholar
Bellali S., Lagier J.C., Raoult D., Bou Khalil J.: Among Live and Dead Bacteria, the Optimization of Sample Collection and Processing Remains Essential in Recovering Gut Microbiota Components. Front Microbiol10, 1606 (2019)BellaliS.LagierJ.C.RaoultD.Bou KhalilJ.: Among Live and Dead Bacteria, the Optimization of Sample Collection and Processing Remains Essential in Recovering Gut Microbiota Components. Front Microbiol10, 1606 (2019)Search in Google Scholar
Biclot A., Huys G.R.B., Bacigalupe R., D’hoe K., Vandeputte D., Falony G., Tito R.Y., Raes J.: Effect of cryopreservation medium conditions on growth and isolation of gut anaerobes from human faecal samples. Microbiome10(1), 80 (2022)BiclotA.HuysG.R.B.BacigalupeR.D’hoeK.VandeputteD.FalonyG.TitoR.Y.RaesJ.: Effect of cryopreservation medium conditions on growth and isolation of gut anaerobes from human faecal samples. Microbiome10(1), 80 (2022)Search in Google Scholar
Bilinski J. & Dziurzynski M. et al.: Fresh Versus Frozen Stool for Fecal Microbiota Transplantation-Assessment by Multimethod Approach Combining Culturing, Flow Cytometry, and Next Generation Sequencing. Front. Microbiol. 13, 872735 (2022)BilinskiJ.DziurzynskiM.: Fresh Versus Frozen Stool for Fecal Microbiota Transplantation-Assessment by Multimethod Approach Combining Culturing, Flow Cytometry, and Next Generation Sequencing. Front. Microbiol. 13, 872735 (2022)Search in Google Scholar
Cardona S., Eck A., Cassellas M., Gallart M., Alastrue C., Dore J., Azpiroz F., Roca J., Guarner F., Manichanh C.: Storage conditions of intestinal microbiota matter in metagenomic analysis. BMC Microbiol12, 158 (2012)CardonaS.EckA.CassellasM.GallartM.AlastrueC.DoreJ.AzpirozF.RocaJ.GuarnerF.ManichanhC.: Storage conditions of intestinal microbiota matter in metagenomic analysis. BMC Microbiol12, 158 (2012)Search in Google Scholar
Chen C.C., Wu W.K., Chang C.M., Panyod S., Lu T.P., Liou J.M., Fang Y.J., Chuang E.Y., Wu M.S.: Comparison of DNA stabilizers and storage conditions on preserving fecal microbiota profiles. J. Formos. Med. Assoc. 119(12), 1791–1798 (2020)ChenC.C.WuW.K.ChangC.M.PanyodS.LuT.P.LiouJ.M.FangY.J.ChuangE.Y.WuM.S.: Comparison of DNA stabilizers and storage conditions on preserving fecal microbiota profiles. J. Formos. Med. Assoc. 119(12), 1791–1798 (2020)Search in Google Scholar
Coppola L., Cianflone A., Grimaldi A.M., Incoronato M., Bevilacqua P., Messina F., Baselice S., Soricelli A., Mirabelli P., Salvatore M.: Biobanking in health care: evolution and future directions. J Transl Med. 17(1), 172 (2019)CoppolaL.CianfloneA.GrimaldiA.M.IncoronatoM.BevilacquaP.MessinaF.BaseliceS.SoricelliA.MirabelliP.SalvatoreM.: Biobanking in health care: evolution and future directions. J Transl Med.17(1), 172 (2019)Search in Google Scholar
Coryell M.P. & Iakiviak M. et al.: A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study. Lancet Microbe, 2(6), 259–266 (2021)CoryellM.P.IakiviakM.: A method for detection of SARS-CoV-2 RNA in healthy human stool: a validation study. Lancet Microbe, 2(6), 259–266 (2021)Search in Google Scholar
Cunningham J.L., Bramstång L., Singh A., Jayarathna S., Rasmusson A.J., Moazzami A., Müller B.: Impact of time and temperature on gut microbiota and SCFA composition in stool samples. PLoS One, 15(8), 0236944 (2020)CunninghamJ.L.BramstångL.SinghA.JayarathnaS.RasmussonA.J.MoazzamiA.MüllerB.: Impact of time and temperature on gut microbiota and SCFA composition in stool samples. PLoS One, 15(8), 0236944 (2020)Search in Google Scholar
Dagher G.: Quality matters: International standards for biobanking. Cell Prolif55(8) 13282 (2022)DagherG.: Quality matters: International standards for biobanking. Cell Prolif55(8) 13282 (2022)Search in Google Scholar
Deschamps C. & Fournier E. et al.: Comparative methods for fecal sample storage to preserve gut microbial structure and function in an in vitro model of the human colon. Appl Microbiol Biotechnol104(23), 10233–10247 (2020)DeschampsC.FournierE.: Comparative methods for fecal sample storage to preserve gut microbial structure and function in an in vitro model of the human colon. Appl Microbiol Biotechnol104(23), 10233–10247 (2020)Search in Google Scholar
DNA Genotek. PD-PR-01036. Storage recommendations for samples collected using Omnigene products. Available at: https://www.dnagenotek.com. (2010)DNA Genotek. PD-PR-01036. Storage recommendations for samples collected using Omnigene products. Available at: https://www.dnagenotek.com. (2010)Search in Google Scholar
Doukhaine E., Gamwell L., Bouevitch A., Elgoff C., Lynch D., Smith C., Del Duca A., Odouli R., Ferber J., Li D., Merino C.: Critical To Quality pre-analytical factors and their impact on microbiome analysis. Available at: https://www.dnagenotek. com/us/pdf/Critical-To-Quality-pre-analytical-factors-and-their-impact-on-microbiome-analysis-MK-00629.pdf. (2021)DoukhaineE.GamwellL.BouevitchA.ElgoffC.LynchD.SmithC.Del DucaA.OdouliR.FerberJ.LiD.MerinoC.: Critical To Quality pre-analytical factors and their impact on microbiome analysis. Available at: https://www.dnagenotek.com/us/pdf/Critical-To-Quality-pre-analytical-factors-and-their-impact-on-microbiome-analysis-MK-00629.pdf. (2021)Search in Google Scholar
Flores R., Shi J., Yu G., Ma B., Ravel J., Goedert J.J., Sinha R.: Collection media and delayed freezing effects on microbial composition of human stool. Microbiome3, 33 (2015)FloresR.ShiJ.YuG.MaB.RavelJ.GoedertJ.J.SinhaR.: Collection media and delayed freezing effects on microbial composition of human stool. Microbiome3, 33 (2015)Search in Google Scholar
Guan H. & Pu Y. et al.: Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabolomics. mSphere, 6(5), e0063621 (2021)GuanH.PuY.: Comparison of Fecal Collection Methods on Variation in Gut Metagenomics and Untargeted Metabo-lomics. mSphere, 6(5), e0063621 (2021)Search in Google Scholar
Hale V.L., Tan C.L., Knight R., Amato K.R.: Effect of preservation method on spider monkey (Ateles geoffroyi) fecal microbiota over 8 weeks. J Microbial Methods. 113, 16–26 (2015)HaleV.L.TanC.L.KnightR.AmatoK.R.: Effect of preservation method on spider monkey (Ateles geoffroyi) fecal microbiota over 8 weeks. J Microbial Methods.113, 16–26 (2015)Search in Google Scholar
Hickl O., Heintz-Buschart A., Trautwein-Schult A., Hercog R., Bork P., Wilmes P., Becher D.: Sample Preservation and Storage Significantly Impact Taxonomic and Functional Profiles in Metaproteomics Studies of the Human Gut Microbiome. Microorganisms, 7(9), 367 (2019)HicklO.Heintz-BuschartA.Trautwein-SchultA.HercogR.BorkP.WilmesP.BecherD.: Sample Preservation and Storage Significantly Impact Taxonomic and Functional Profiles in Metaproteomics Studies of the Human Gut Microbiome. Microorganisms, 7(9), 367 (2019)Search in Google Scholar
Kawada Y., Naito Y., Andoh A., Ozeki M., Inoue R.: Effect of storage and DNA extraction method on 16S rRNA-profiled fecal microbiota in Japanese adults. J Clin Biochem Nutr. 64(2), 106–111 (2019)KawadaY.NaitoY.AndohA.OzekiM.InoueR.: Effect of storage and DNA extraction method on 16S rRNA-profiled fecal microbiota in Japanese adults. J Clin Biochem Nutr. 64(2), 106–111 (2019)Search in Google Scholar
Li X.M., Shi X., Yao Y., Shen Y.C., Wu X.L., Cai T., Liang L.X., Wang F.: Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis. Microbiol Spectr11(3), e0429722 (2023)LiX.M.ShiX.YaoY.ShenY.C.WuX.L.CaiT.LiangL.X.WangF.: Effects of Stool Sample Preservation Methods on Gut Microbiota Biodiversity: New Original Data and Systematic Review with Meta-Analysis. Microbiol Spectr11(3), e0429722 (2023)Search in Google Scholar
Liang Y. & Dong T. et al.: Systematic Analysis of Impact of Sampling Regions and Storage Methods on Fecal Gut Microbiome and Metabolome Profiles. mSphere, 5(1), e00763–1 (2020)LiangY.DongT.: Systematic Analysis of Impact of Sampling Regions and Storage Methods on Fecal Gut Microbiome and Metabolome Profiles. mSphere, 5(1), e00763–1 (2020)Search in Google Scholar
Life Technologies. RNAlater® tissue collection: RNA stabilizationnsolution. Available from: https://tools.thermofisher.com/content/sfs/manuals/cms_056069.pdf (2011)Life Technologies. RNAlater® tissue collection: RNA stabiliza-tionnsolution. Available from: https://tools.thermofisher.com/content/sfs/manuals/cms_056069.pdf (2011)Search in Google Scholar
Mykytczuk N.C., Foote S.J., Omelon C.R., Southam G., Greer C.W., Whyte L.G.: Bacterial growth at–15 °C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1. ISME J. 7(6), 1211–1226 (2013)MykytczukN.C.FooteS.J.OmelonC.R.SouthamG.GreerC.W.WhyteL.G.: Bacterial growth at-15 °C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1. ISME J.7(6), 1211–1226 (2013)Search in Google Scholar
Neuberger-Castillo L., Ammerlaan W., Betsou F.: Fitness for purpose of stabilized stool samples for bile acid metabolite analyses. Sci Rep. 11(1), 7904 (2021)Neuberger-CastilloL.AmmerlaanW.BetsouF.: Fitness for purpose of stabilized stool samples for bile acid metabolite analyses. Sci Rep.11(1), 7904 (2021)Search in Google Scholar
Neuberger-Castillo L., Hamot G., Marchese M., Sanchez I., Ammerlaan W., Betsou F.: Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis. Biopreserv Biobank, 18(2), 102–116 (2020)Neuberger-CastilloL.HamotG.MarcheseM.SanchezI.AmmerlaanW.BetsouF.: Method Validation for Extraction of DNA from Human Stool Samples for Downstream Microbiome Analysis. Biopreserv Biobank, 18(2), 102–116 (2020)Search in Google Scholar
OECD, Organization for Economic Co-operation and Development (OECD): OECD Series on Principles of Good Laboratory Practice and Compliance Monitoring Number 14: Advisory Document of the Working Group on Good Laboratory Practice – The Application of the Principles of GLP to in vitro Studies. OECD Publishing (2004)OECD, Organization for Economic Co-operation and Development (OECD): OECD Series on Principles of Good Laboratory Practice and Compliance Monitoring Number 14: Advisory Document of the Working Group on Good Laboratory Practice – The Application of the Principles of GLP to in vitro Studies. OECD Publishing (2004)Search in Google Scholar
O’Sullivan V., Madrid-Gambin F., Alegra T., Gibbons H., Brennan L.: Impact of Sample Storage on the NMR Fecal Water Metabolome. ACS Omega, 3(12), 16585–16590 (2018)O’SullivanV.Madrid-GambinF.AlegraT.GibbonsH.BrennanL.: Impact of Sample Storage on the NMR Fecal Water Metabolome. ACS Omega, 3(12), 16585–16590 (2018)Search in Google Scholar
Park C., Yun K.E., Chu J.M., Lee J.Y., Hong C.P., Nam Y.D., Jeong J., Han K., Ahn Y.J.: Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature. J Microbiol. 58(8), 703–710 (2020)ParkC.YunK.E.ChuJ.M.LeeJ.Y.HongC.P.NamY.D.JeongJ.HanK.AhnY.J.: Performance comparison of fecal preservative and stock solutions for gut microbiome storage at room temperature. J Microbiol.58(8), 703–710 (2020)Search in Google Scholar
Santiago A., Panda S., Mengels G., Martinez X., Azpiroz F., Dore J., Guarner F., Manichanh C.: Processing faecal samples: a step forward for standards in microbial community analysis. BMC Microbiol. 14, 112 (2014)SantiagoA.PandaS.MengelsG.MartinezX.AzpirozF.DoreJ.GuarnerF.ManichanhC.: Processing faecal samples: a step forward for standards in microbial community analysis. BMC Microbiol. 14, 112 (2014)Search in Google Scholar
Song S.J., Amir A., Metcalf J.L., Amato K.R., Xu Z.Z., Humphrey G., Knight R.: Preservation Methods Differ in Fecal Microbiome Stability, Affecting Suitability for Field Studies. mSystems1(3), e00021–16 (2016)SongS.J.AmirA.MetcalfJ.L.AmatoK.R.XuZ.Z.HumphreyG.KnightR.: Preservation Methods Differ in Fecal Microbiome Stability, Affecting Suitability for Field Studies. mSystems1(3), e00021–16 (2016)Search in Google Scholar
Souza Y.F.V.P., Souza E.V., Azevedo L.S., Medeiros R.S., Timenetsky M.D.C.S.T., Luchs A.: Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998–2005): Pre-rotavirus vaccine era. Infect Genet Evol. 94, 105007 (2021)SouzaY.F.V.P.SouzaE.V.AzevedoL.S.MedeirosR.S.TimenetskyM.D.C.S.T.LuchsA.: Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998-2005): Pre-rotavirus vaccine era. Infect Genet Evol. 94, 105007 (2021)Search in Google Scholar
Stumptner C., Stadlbauer V., O’Neil D., Gessner A., Hiergeist A., Zatloukal K., Abuja P.M.: The Pre-Analytical CEN/TS Standard for Microbiome Diagnostics-How Can Research and Development Benefit? Nutrients, 14(9), 1976 (2022)StumptnerC.StadlbauerV.O’NeilD.GessnerA.HiergeistA.ZatloukalK.AbujaP.M.: The Pre-Analytical CEN/TS Standard for Microbiome Diagnostics-How Can Research and Development Benefit?Nutrients, 14(9), 1976 (2022)Search in Google Scholar
Swidsinski A., Loening-Baucke V., Kirsch S., Doerffel Y.: Biostructure fonctionnelle du microbiote colique(zone de fermentation centrale, zone de réservegerminale et couche de mucus séparatrice) chez lessujets sains et chez les patients atteints de diarrhéetraités par Saccharomyces boulardii [Functional biostructure of colonic microbiota (central fermenting area, germinal stock area and separating mucus layer) in healthy subjects and patients with diarrhea treated with Saccharomyces boulardii]. Gastroenterol Clin Biol34 Suppl 1, 79–92 (2010)SwidsinskiA.Loening-BauckeV.KirschS.DoerffelY.: Biostructure fonctionnelle du microbiote colique(zone de fermentation centrale, zone de réservegerminale et couche de mucus séparatrice) chez lessujets sains et chez les patients atteints de diarrhéetraités par Saccharomyces boulardii [Functional biostructure of colonic microbiota (central fermenting area, germinal stock area and separating mucus layer) in healthy subjects and patients with diarrhea treated with Saccharomyces boulardii]. Gastroenterol Clin Biol34Suppl 1, 79–92 (2010)Search in Google Scholar
Tamada H., Ito Y., Ebara T., Kato S., Kaneko K., Matsuki T., Sugiura-Ogasawara M., Saitoh S., Kamijima M.: Epidemiological Studies of Children’s Gut Microbiota: Validation of Sample Collection and Storage Methods and Microbiota Analysis of Toddlers’ Feces Collected from Diapers. Nutrients, 14(16), 3315 (2022)TamadaH.ItoY.EbaraT.KatoS.KanekoK.MatsukiT.Sugiura-OgasawaraM.SaitohS.KamijimaM.: Epidemiological Studies of Children’s Gut Microbiota: Validation of Sample Collection and Storage Methods and Microbiota Analysis of Toddlers’ Feces Collected from Diapers. Nutrients, 14(16), 3315 (2022)Search in Google Scholar
Tap J., Cools-Portier S., Pavan S., Druesne A., Öhman L., Törnblom H., Simren M., Derrien M.: Effects of the long-term storage of human fecal microbiota samples collected in RNAl-ater. Sci Rep. 9(1), 601 (2019)TapJ.Cools-PortierS.PavanS.DruesneA.ÖhmanL.TörnblomH.SimrenM.DerrienM.: Effects of the long-term storage of human fecal microbiota samples collected in RNAl-ater. Sci Rep.9(1), 601 (2019)Search in Google Scholar
Thomas V., Clark J., Doré J.: Fecal microbiota analysis: an overview of sample collection methods and sequencing strategies. Future Microbiol. 10(9), 1485–504 (2015)ThomasV.ClarkJ.DoréJ.: Fecal microbiota analysis: an overview of sample collection methods and sequencing strategies. Future Microbiol. 10(9), 1485–504 (2015)Search in Google Scholar
Widjaja F., Rietjens I.M.C.M.: From-Toilet-to-Freezer: A Review on Requirements for an Automatic Protocol to Collect and Store Human Fecal Samples for Research Purposes. Biomedicines, 11(10), 2658 (2023)WidjajaF.RietjensI.M.C.M.: From-Toilet-to-Freezer: A Review on Requirements for an Automatic Protocol to Collect and Store Human Fecal Samples for Research Purposes. Biomedicines, 11(10), 2658 (2023)Search in Google Scholar
Williams G.M., Leary S.D., Ajami N.J., Chipper Keating S., Petrosin J.F., Hamilton-Shield J.P., Gillespie K.M.: Gut microbiome analysis by post: Evaluation of the optimal method to collect stool samples from infants within a national cohort study. PLoS One14(6), e0216557 (2019)WilliamsG.M.LearyS.D.AjamiN.J.Chipper KeatingS.PetrosinJ.F.Hamilton-ShieldJ.P.GillespieK.M.: Gut microbiome analysis by post: Evaluation of the optimal method to collect stool samples from infants within a national cohort study. PLoS One14(6), e0216557 (2019)Search in Google Scholar
Wirth U. & Garzetti D. et al.: Microbiome Analysis from Paired Mucosal and Fecal Samples of a Colorectal Cancer Biobank. Cancers (Basel), 12(12), 3702 (2020)WirthU.GarzettiD.: Microbiome Analysis from Paired Mucosal and Fecal Samples of a Colorectal Cancer Biobank. Cancers (Basel), 12(12), 3702 (2020)Search in Google Scholar
Wu C. & Chen T. et al.: The maintenance of microbial community in human fecal samples by a cost effective preservation buffer. Sci Rep. 11, 13453 (2021)WuC.ChenT.: The maintenance of microbial community in human fecal samples by a cost effective preservation buffer. Sci Rep. 11, 13453 (2021)Search in Google Scholar
Yang S., He Y., Zhang J., Zhang D., Wang Y., Lu X., Wang X., Shen Q., Ji L., Lu H., Zhang W.: Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea. Virol Sin. 37(1), 82–93 (2022)YangS.HeY.ZhangJ.ZhangD.WangY.LuX.WangX.ShenQ.JiL.LuH.ZhangW.: Viral metagenomics reveals diverse viruses in the fecal samples of children with diarrhea. Virol Sin.37(1), 82–93 (2022)Search in Google Scholar
Young R.R., Jenkins K., Araujo-Perez F., Seed P.C., Kelly M.S.: Long-term stability of microbiome diversity and composition in fecal samples stored in eNAT medium. Microbiologyopen, 9(7), e1046 (2020)YoungR.R.JenkinsK.Araujo-PerezF.SeedP.C.KellyM.S.: Long-term stability of microbiome diversity and composition in fecal samples stored in eNAT medium. Microbiologyopen, 9(7), e1046 (2020)Search in Google Scholar