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Gonococci – Pathogens of Growing Importance. Part 1. Current Data on Diagnostics, Genotyping and Therapy.

Joanna Białecka

Joanna Białecka

  • Centre of Microbiological Research and AutovaccinesCracow, Poland
  • Department of Pharmaceutical Microbiology, Jagiellonian University Medical CollegeCracow, Poland
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Białecka, Joanna
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Katarzyna Rak

Katarzyna Rak

Centre of Microbiological Research and AutovaccinesCracow, Poland
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Rak, Katarzyna
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Aneta Kiecka

Aneta Kiecka

  • Centre of Microbiological Research and AutovaccinesCracow, Poland
  • Chair of Biomedical Sciences, Institute of Physiotherapy, Jagiellonian University Medical CollegeCracow, Poland
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Kiecka, Aneta
  
25 mar 2024
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Pubblicato online: 25 mar 2024
Pagine: 3 - 14
Ricevuto: 01 gen 2024
Accettato: 01 feb 2024
DOI: https://doi.org/10.2478/am-2024-0001
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© 2024 Joanna Białecka et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Methods used in the diagnosis of N. gonorrhoeae.
Methods used in the diagnosis of N. gonorrhoeae.

MIC breakpoint range for N_ gonorrhoeae according to EUCAST recommendations v 12_0 2023_01_01 and CLSI 2020

Antibiotic (abbreviation) MIC limit values (mg/l)
EUCAST v 12.0 2023 CLSI 2020
≤ W > O ≤ W > O
Penicillin (P) 0,06 1,0 0,06 2,0
Cefixime (CFM) 0,125 0,125 0,25 -
Ceftriaxone (CRO) 0,125 0,125 0,25 -
Cefotaxime (CTX) 0,125 0,125 0,5 -
Azithromycin (AZM)* 1,0 (ECOFF) 1,0 -
Ciprofloxacin (CIP) 0,03 0,06 0,06 1,0
Tetracycline (T) 0,5 0,5 0,25 2,0
Spectinomycin (SPT) 64,0 64,0 32,0 128,0

FDA-approved molecular diagnostic tests for the clinical diagnosis of gonorrhea, based on CDC Recommendations (2014)

NAAT test detected pathogens Producer Method Molecular targets N. gonorrhoeae
Aptima NG assay N. gonorrhoeae (Hologic/Gen-Probe, SanDiego, California) TMA Specific region of 16S rRNA, different than in the Aptima Combo 2 assay
Aptima Combo 2 assay C. trachomatis, N. gonorrhoeae (Hologic/Gen-Probe, SanDiego, California) TMA Specific region of 16S rRNA
Abbott Real Time 2000 CT/NG C. trachomatis, N. gonorrhoeae Abbott Molecular Inc. Des Plaines, Illinois qPCR 48 bp sequence within the Opa gene
Cobas CT/NG C. trachomatis, N. gonorrhoeae Roche Molecular Diagnostics, Branchburg, New Jersey PCR two sequences within the fragment DR 9A and DR 9B (DR – direct repeat)
BD ProbeTec ET CT/GC Ampliefied DNA assay C. trachomatis, N. gonorrhoeae Becton Dickinson, Sparks, Maryland SDA Chromosomal pilin gene-inverting protein homolog
BD ProbeTec NG Q N. gonorrhoeae Becton Dickinson, Sparks, Maryland SDA Chromosomal pilin gene-inverting protein homolog
Xpert CT/NG Assay C. trachomatis, N. gonorrhoeaes Cepheid, Sunnyvale, California qPCR two specific chromosomal DNA sequence
Lingue:
Inglese, Polacco
Frequenza di pubblicazione:
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Argomenti della rivista:
Scienze biologiche, Microbiologia e virologia
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