Challenges to antimicrobial susceptibility testing of plant-derived polyphenolic compounds
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31 dic 2020
INFORMAZIONI SU QUESTO ARTICOLO
Categoria dell'articolo: Review
Pubblicato online: 31 dic 2020
Pagine: 300 - 311
Ricevuto: 01 feb 2020
Accettato: 01 ott 2020
DOI: https://doi.org/10.2478/aiht-2020-71-3396
Parole chiave
© 2020 Marina Bubonja-Šonje et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Overview of commonly used in vitro antimicrobial susceptibility testing methods
| Method | Advantages | Disadvantages | Reference |
|---|---|---|---|
| Agar disk diffusion | simplicity of performance, low cost, flexibility, no special equipment required, suitable for lead identification | qualitative assay, poor level of reproducibility, diffusion of antimicrobial substances may be affected, applicable only to fast-growing bacteria | ( |
| Agar well diffusion | simplicity of performance, low cost, more sensitive and more convenient than the disc variant for testing of cationic compounds | qualitative assay, poor level of reproducibility | ( |
| Bioautography | simplicity of performance, little amount of sample required, rapid and inexpensive evaluation, suitable for screening of antimicrobials in mixtures | qualitative assay, difficult to standardise, not suitable for synergy studies, alteration of compounds during the fractional phase | ( |
| Agar dilution | quantitative results, a number of bacterial species may be applied to a single dish | laborious and time consuming method, the large amount of reagents and space required | ( |
| Broth microdilution | quantitative results, convenience and time/cost effectiveness, capacity to test opaque materials, possible automation, the most consistent results, the killing effect can be assessed | the possibility of errors in solution preparation, relatively high amount of space and reagents required | ( |
| Microfluidic methods | smaller volumes, short run time, higher sensitivity, potential for high throughput | specialised equipment needed, high-cost | ( |