Accesso libero

Costal cartilage ensures low degradation of DNA needed for genetic identification of human remains retrieved at different decomposition stages and different postmortem intervals

Marcin Tomsia
Marcin Tomsia
, 
Kornelia Droździok
Kornelia Droździok
, 
Gulnaz T. Javan
Gulnaz T. Javan
, 
Rafał Skowronek
Rafał Skowronek
, 
Michał Szczepański
Michał Szczepański
 e 
Elżbieta Chełmecka
Elżbieta Chełmecka
   | 04 dic 2021
Postępy Higieny i Medycyny Doświadczalnej's Cover Image
INFORMAZIONI SU QUESTO ARTICOLO
Articolo precedente
Articolo Successivo
Cita
Article Category: Review
Pubblicato online: 04 dic 2021
Pagine: 852 - 858
Ricevuto: 07 dic 2020
Accettato: 24 giu 2021
DOI: https://doi.org/10.2478/ahem-2021-0035
Parole chiave
© 2021 Tomsia et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1

Anatomical location of costal cartilage in the rib cage (light gray color). The arrow shows the place from which the research material was collected
Anatomical location of costal cartilage in the rib cage (light gray color). The arrow shows the place from which the research material was collected

Figure 2

Morphological changes in costal cartilage at different times elapsed since death and at different exposure times to environmental factors: A – few days after death; B – few weeks after death; C – 7 months after death; D – 2 years and 11 months after death
Morphological changes in costal cartilage at different times elapsed since death and at different exposure times to environmental factors: A – few days after death; B – few weeks after death; C – 7 months after death; D – 2 years and 11 months after death

Figure 3

Morphological changes in costal cartilage in almost skeletonized cadavers: A – remains of costal cartilage collected from 67-year-old male cadaver of unknown identity, found in the converted and abandoned bus 14 months after death; B – remains of costal cartilage collected from male cadaver of unknown identity and age, found in a ditch 3 years after death
Morphological changes in costal cartilage in almost skeletonized cadavers: A – remains of costal cartilage collected from 67-year-old male cadaver of unknown identity, found in the converted and abandoned bus 14 months after death; B – remains of costal cartilage collected from male cadaver of unknown identity and age, found in a ditch 3 years after death

Figure 4

Places in which the cadavers included in the study were found
Places in which the cadavers included in the study were found

Figure 5

DNA concentration [ng/ μl] after T. Large autosomal sequence (214 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments
DNA concentration [ng/ μl] after T. Large autosomal sequence (214 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments

Figure 6

DNA concentration [ng/ μl] after T. Small autosomal sequence (80 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments
DNA concentration [ng/ μl] after T. Small autosomal sequence (80 bp) amplification in costal cartilage samples collected from cadavers retrieved from different environments

Figure 7

DNA concentration [ng/ μl] after the Y chromosome sequence (75 bp) amplification in costal cartilage samples collected from male cadavers retrieved from different environments
DNA concentration [ng/ μl] after the Y chromosome sequence (75 bp) amplification in costal cartilage samples collected from male cadavers retrieved from different environments

Figure 8

DNA degradation index* in costal cartilage samples collected from cadavers retrieved from different environments. *T. Large amplicon DNA concentration/T. Small amplicon DNA concentration
DNA degradation index* in costal cartilage samples collected from cadavers retrieved from different environments. *T. Large amplicon DNA concentration/T. Small amplicon DNA concentration

Statistical analysis of DNA concentration measured for different loci in costal cartilage samples collected from cadavers retrieved from different environments. The results are presented as median (lower – upper quartile). Statistical significance was set at p > 0.05

Cadaver retrieval location
Variable 1 Indoors (flat/after hospitalization) n = 19 2 Outdoors (primarily in forest area) n = 24 3 Water/exhumation site n = 5 4 Coal mine n = 9 5 Fire site n = 6 6 Basement/uninhabited building n = 7 7 Unknown location n = 9 p
T. Large amplicon DNA concentration [ng/μl] n = 79 4.8 (2.6-8.7) 4.8 (2.5-9.7) 8.4 (3.9-14.1) 9.1 (7.6-11.6) 4.6 (2.3-13.7) 6.5 (4.8-7.3) 6.2 (4.4-19.5) 0.143
T. Small amplicon DNA concentration [ng/μl] n = 79 3.8 (2.3-8.2) 3.8 (2.3-7.1) 7.1 (2.5-13.4) 7.1(6.2-9.2) 4.9 (2.5-10.0) 6.6 (4.3-7.8) 4.8 (3.7-16.7) 0.146
Y amplicon DNA concentration [ng/μl] n = 68 6.0 (3.9-9.8) n = 16 4.3 (2.3-7.1) n = 22 9.3 (3.2-17.2) n = 4 8.7 (7.2-13.3) n = 9 9.5 (5.4-14.9) n = 4 6.4 (8.8-7.9) n = 6 10.2 (4.4-21.3) n = 7 0.132
Degradation index* n = 79 0.9 (0.8-1.0) 0.8 (0.7-0.9) 0.9 (0.6-0.9) 0.9 (0.8-0.9) 1.0 (0.8-1.1) 1.0 (0.7-1.1) 0.8 (0.8-0.9) 0.503
eISSN:
1732-2693
Lingua:
Inglese
Frequenza di pubblicazione:
Volume Open
Argomenti della rivista:
Life Sciences, Molecular Biology, Microbiology and Virology, Medicine, Basic Medical Science, Immunology
Feed RSS della rivista