<abstract xmlns="http://www.w3.org/1999/xhtml">

African swine fever continues to pose a global agricultural problem due to the absence of vaccine prevention and the high cost of anti-epizootic measures. This study examines the functional role of the African swine fever virus (ASFV) 1L-5-6L multigene family 110 genes <italic>in vivo</italic>. Four clinically healthy Large White pigs were used in this study. Two groups of animals were inoculated with either the parental strain or the deletion variant, respectively. For subsequent challenge infection, the homologous virulent strain “Stavropol 01/08” was used. Blood samples were collected at specific time intervals. The ASFV infectious activity was determined by titration in porcine blood-derived macrophages. Virus-specific antibodies to the ASFV p30 protein were detected using an enzyme-linked immunosorbent assay (ELISA).
The results of real-time polymerase chain reaction (PCR) showed a significant difference in Ct values between samples from the two groups of animals. The determination of ASFV infectious activity in blood samples demonstrated the presence of the virus in animals immunized with the parental strain. The virus was not detected in samples from animals immunized with the deletion strain. The ELISA method demonstrated the presence of p30 protein antibodies in serum samples from 10 to 14 days after immunization with the parental strain, while no antibodies were detected in serum samples from animals immunized with the deletion strain.
The properties of the ASFV recombinant strain “Volgograd/D(1L-5-6L) MGF110” were studied in an <italic>in vivo</italic> experiment. It was found that the deletion strain does not reproduce in animals, unlike the parental strain.
</abstract>

African swine fever continues to pose a global agricultural problem due to the absence of vaccine prevention and the high cost of anti-epizootic measures. This study examines the functional role of the African swine fever virus (ASFV) 1L-5-6L multigene family 110 genes in vivo. Four clinically healthy Large White pigs were used in this study. Two groups of animals were inoculated with either the parental strain or the deletion variant, respectively. For subsequent challenge infection, the homologous virulent strain “Stavropol 01/08” was used. Blood samples were collected at specific time intervals. The ASFV infectious activity was determined by titration in porcine blood-derived macrophages. Virus-specific antibodies to the ASFV p30 protein were detected using an enzyme-linked immunosorbent assay (ELISA).
The results of real-time polymerase chain reaction (PCR) showed a significant difference in Ct values between samples from the two groups of animals. The determination of ASFV infectious activity in blood samples demonstrated the presence of the virus in animals immunized with the parental strain. The virus was not detected in samples from animals immunized with the deletion strain. The ELISA method demonstrated the presence of p30 protein antibodies in serum samples from 10 to 14 days after immunization with the parental strain, while no antibodies were detected in serum samples from animals immunized with the deletion strain.
The properties of the ASFV recombinant strain “Volgograd/D(1L-5-6L) MGF110” were studied in an in vivo experiment. It was found that the deletion strain does not reproduce in animals, unlike the parental strain.

Determination of the 1L-5-6L MGF110 Genes Influence on the Biological Properties of the African Swine Fever Virus (<italic>Asfarviridae; Asfivirus</italic>) “Volgograd/14c” <italic>in vivo</italic>

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Determination of the 1L-5-6L MGF110 Genes Influence on the Biological Properties of the African Swine Fever Virus () “Volgograd/14c” 

African swine fever continues to pose a global agricultural problem due to the absence of vaccine prevention and the high cost of anti-epizootic measures. This...

Determination of the 1L-5-6L MGF110 Genes Influence on the Biological Properties of the African Swine Fever Virus (Asfarviridae; Asfivirus) “Volgograd/14c” in vivo

Article Category: Research article

Pubblicato online: 24 giu 2024

Pagine: 210 - 221

Ricevuto: 22 feb 2024

Accettato: 07 giu 2024

DOI: https://doi.org/10.2478/acve-2024-0014

Parole chiave
African swine fever virus, multigenic family 110 (MGF110), PCR, ELISA

© 2024 Mikhail E. Vlasov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Determination of the 1L-5-6L MGF110 Genes Influence on the Biological Properties of the African Swine Fever Virus (Asfarviridae; Asfivirus) “Volgograd/14c” in vivo

Article Category: Research article

Pubblicato online: 24 giu 2024

Pagine: 210 - 221

Ricevuto: 22 feb 2024

Accettato: 07 giu 2024

DOI: https://doi.org/10.2478/acve-2024-0014

Parole chiaveAfrican swine fever virus, multigenic family 110 (MGF110), PCR, ELISA

© 2024 Mikhail E. Vlasov et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Parole chiave
African swine fever virus, multigenic family 110 (MGF110), PCR, ELISA