<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Ovine respiratory complex is a significant cause of death in sheep flocks, where <italic>Pasteurella multocida</italic> is the most frequent microorganisms isolated from animals with pneumonia. There is an urgent need to refine the use of different antimicrobials to avoid the problem of antimicrobial resistance and optimize the control of this disease in ovine livestock. The first step in approaching this problem is gaining an insight into the antimicrobial susceptibility of ovine pathogens. This study evaluated the <italic>in vitro</italic> activity of tildipirosin, gamithromycin, oxytetracycline, and danofloxacin against <italic>Pasteurella multocida</italic> strains isolated from sheep with fibrinous pneumonia. The strains were incubated following Clinical and Laboratory Standards Institute (CLSI) standard conditions and also with a modified method by 25% supplementation with sheep serum. Minimum inhibitory concentrations (MIC) were determined using the broth microdilution technique. The lowest MIC<sub>90</sub> under standard conditions and by supplementation with sheep serum was obtained with tildipirosin. Sheep serum significantly reduced tildipirosin, gamithromycin, and danofloxacin MIC values for <italic>Pasteurella multocida</italic> strains. In brief, the potency of tildipirosine, gamithromycin, and danofloxacin against <italic>Pasteurella multocida</italic> increases when sheep serum is added to the culture media.</p>
</abstract>

Ovine respiratory complex is a significant cause of death in sheep flocks, where Pasteurella multocida is the most frequent microorganisms isolated from animals with pneumonia. There is an urgent need to refine the use of different antimicrobials to avoid the problem of antimicrobial resistance and optimize the control of this disease in ovine livestock. The first step in approaching this problem is gaining an insight into the antimicrobial susceptibility of ovine pathogens. This study evaluated the in vitro activity of tildipirosin, gamithromycin, oxytetracycline, and danofloxacin against Pasteurella multocida strains isolated from sheep with fibrinous pneumonia. The strains were incubated following Clinical and Laboratory Standards Institute (CLSI) standard conditions and also with a modified method by 25% supplementation with sheep serum. Minimum inhibitory concentrations (MIC) were determined using the broth microdilution technique. The lowest MIC90 under standard conditions and by supplementation with sheep serum was obtained with tildipirosin. Sheep serum significantly reduced tildipirosin, gamithromycin, and danofloxacin MIC values for Pasteurella multocida strains. In brief, the potency of tildipirosine, gamithromycin, and danofloxacin against Pasteurella multocida increases when sheep serum is added to the culture media.

Antimicrobial Susceptibility of <italic>Pasteurella multocida</italic> Isolated from Sheep with Fibrinous Pneumonia

Escuela de Medicina Veterinaria. Colegio de Ciencias de la Salud. Universidad San Francisco de Quito

Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare Nostrum”

Acta Veterinaria

This work is licensed under the Creative Commons Attribution 4.0 International License.

Antimicrobial Susceptibility of  Isolated from Sheep with Fibrinous Pneumonia

Ovine respiratory complex is a significant cause of death in sheep flocks, where Pasteurella multocida is the most frequent microorganisms isolated from...

Antimicrobial Susceptibility of Pasteurella multocida Isolated from Sheep with Fibrinous Pneumonia

Article Category: Research article

Pubblicato online: 01 lug 2023

Pagine: 171 - 178

DOI: https://doi.org/10.2478/acve-2023-0013

Parole chiave
Antimicrobial susceptibility, pneumonia, sheep,

© 2023 Juan Sebastián Galecio et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Antimicrobial Susceptibility of Pasteurella multocida Isolated from Sheep with Fibrinous Pneumonia

Article Category: Research article

Pubblicato online: 01 lug 2023

Pagine: 171 - 178

DOI: https://doi.org/10.2478/acve-2023-0013

Parole chiaveAntimicrobial susceptibility, pneumonia, sheep,

© 2023 Juan Sebastián Galecio et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Parole chiave
Antimicrobial susceptibility, pneumonia, sheep,