<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The in vitro culture of ovarian follicles or cumulus-oocyte complexes (COC) is used to study the factors that regulate follicular development and may have potential use in artificial reproductive technology (ART). Before ovulation, the follicle is formed by oocyte and cell populations known as granulosa cells (GCs). These cells build the internal and external mass of the follicular wall. Oocyte growth and proliferation of the surrounding cells depend on the gap junctions between the oocyte and the GCs. Maintenance of the optimal in vitro culture system allowing for preservation of follicle architecture and granulosa-oocyte interaction may be critical for success in vitro maturation of follicles. Recently many studies have focused on a culture of GCs, which have important functions related to steroidogenesis. Granulosa cells maintained in in vitro conditions exhibit stem cell properties making it important to consider in vitro culture (IVC) methods of the GC population.</p>
</abstract>

The in vitro culture of ovarian follicles or cumulus-oocyte complexes (COC) is used to study the factors that regulate follicular development and may have potential use in artificial reproductive technology (ART). Before ovulation, the follicle is formed by oocyte and cell populations known as granulosa cells (GCs). These cells build the internal and external mass of the follicular wall. Oocyte growth and proliferation of the surrounding cells depend on the gap junctions between the oocyte and the GCs. Maintenance of the optimal in vitro culture system allowing for preservation of follicle architecture and granulosa-oocyte interaction may be critical for success in vitro maturation of follicles. Recently many studies have focused on a culture of GCs, which have important functions related to steroidogenesis. Granulosa cells maintained in in vitro conditions exhibit stem cell properties making it important to consider in vitro culture (IVC) methods of the GC population.

Approaches for in vitro culture of granulosa cells and ovarian follicles

University Hospital and Masaryk University

Université Catholique de LouvainBrussels

Department of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences

Department of Basic and Preclinical Sciences, Faculty of Biological and Veterinary Sciences

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Approaches for in vitro culture of granulosa cells and ovarian follicles"}

The in vitro culture of ovarian follicles or cumulus-oocyte complexes (COC) is used to study the factors that regulate follicular development and may have...

Approaches for in vitro culture of granulosa cells and ovarian follicles

Pubblicato online: 10 apr 2022

Pagine: 34 - 42

Ricevuto: 25 gen 2022

Accettato: 04 mar 2022

DOI: https://doi.org/10.2478/acb-2022-0006

Parole chiave
granulosa cells (GCs), cumulus-oocyte complexes (COC), ovarian follicle, ovarian follicle, in vitro culture systems

© 2022 Wiktoria Zgórecka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Approaches for in vitro culture of granulosa cells and ovarian follicles

Pubblicato online: 10 apr 2022

Pagine: 34 - 42

Ricevuto: 25 gen 2022

Accettato: 04 mar 2022

DOI: https://doi.org/10.2478/acb-2022-0006

Parole chiavegranulosa cells (GCs), cumulus-oocyte complexes (COC), ovarian follicle, ovarian follicle, in vitro culture systems

© 2022 Wiktoria Zgórecka et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Parole chiave
granulosa cells (GCs), cumulus-oocyte complexes (COC), ovarian follicle, ovarian follicle, in vitro culture systems