<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Multiple Ovulation Embryo Transfer is a biotech method with more than 50 years of history and an established position in cattle breeding. This procedure is beneficial in many ways, but it also carries a risk of failure. The study presents the overview of the most important risk factors that may affect conception rates in the course of embryo transfer, including the factors associated with the embryo sourcing (embryo production method, embryo quality, development stage and breed, embryo storage method), embryo transfer procedure (synchrony/asynchrony, embryo transfer difficulty, the time of the transcervical insemination gun passage, depth of embryo deposition, localization and structure of the corpus luteum relative to the follicle and both individual characteristics of donors and recipients (level of concentration of progesterone, the state of health of the udder, lactation level, body condition score and age) and some environmental factors.</p>
</abstract>

Multiple Ovulation Embryo Transfer is a biotech method with more than 50 years of history and an established position in cattle breeding. This procedure is beneficial in many ways, but it also carries a risk of failure. The study presents the overview of the most important risk factors that may affect conception rates in the course of embryo transfer, including the factors associated with the embryo sourcing (embryo production method, embryo quality, development stage and breed, embryo storage method), embryo transfer procedure (synchrony/asynchrony, embryo transfer difficulty, the time of the transcervical insemination gun passage, depth of embryo deposition, localization and structure of the corpus luteum relative to the follicle and both individual characteristics of donors and recipients (level of concentration of progesterone, the state of health of the udder, lactation level, body condition score and age) and some environmental factors.

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

Department of Basic and Preclinical Sciences, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences

Department of Clinical Sciences and Diagnostics, Institute of Veterinary Medicine, Faculty of Biological and Veterinary Sciences

Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine

Wroclaw University of Environmental and Life Sciences

Medical Journal of Cell Biology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Multiple Ovulation Embryo Transfer is a biotech method with more than 50 years of history and an established position in cattle breeding. This procedure is...

FACTOR	CR
Asynchrony¹
− 48h (5d)	25%
none (7d)	30%
+ 48h (9d)	40%
Embryo transfer difficulty²
Easy	43%
Moderate	32%
Difficult	18%
TTIGP³
10–60s	53.4%
> 60 s	20.4%
Depth of embryo deposition²
Deep in the horn	43%
In the middle part of uterus horn	31%
In the uterus body	24%
Localization of the CL relative to the follicle³
Ipsilaterally	65%
Contrlaterally	33%
Structure of corpus luteum⁴
Cavitary CL	52%
Compact CL	33%

FACTOR	CR FOR THE TRANSFER OF EMBRYOS PRODUCED IN VIVO	CR FOR THE TRANSFER OF EMBRYOS PRODUCED IN VITRO
Embryo production method¹	41.5	33.5
Embryo quality²
Excelent	42.2
Good	32.8
Sufficient	23.7
Embryo development stage²
Morula	36.5
early blastocyst	38.1
Blastocyst	45.3
Embryo breed^3,4
Brangus^3,4	47	36.8
Angus^3,4	39	32.9
Nelore⁴		36.7
HF⁴		22.7
Hereford³	75
Charolaise³	53
Storage method²
Fresh	49.3	39
Frozen	36.8	32.9
Sorting process⁵
Sorter	40	37.3
not sorter	48.7	42.3
Biopsy⁶
Biopsied	54.2	26.4
not biopsied	55.9	19.8

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

Pubblicato online: 27 set 2021

Pagine: 123 - 131

Ricevuto: 13 lug 2021

Accettato: 28 ago 2021

DOI: https://doi.org/10.2478/acb-2021-0017

Parole chiave
embryo transfer, cattle reproduction, assisted reproduction techniques

© 2021 Maria Wieczorkiewicz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of factors related to embryo transfer on the conception rates ((own compilation based on: 1Ledgard et al. [43], 2Roper et al. [16], 3Jaśkowski JM et al. [49] 3Del Campo et al. [13], 4Jaśkowski BM et al. [46]

Effects of factors related to in vivo or in vitro embryo production on conception rates (own compilation based on: 1Pontes et al [5], 2Ferraz et al [6], 3Donaldson et al [21], 4Arreseigor et al [20], 5Mikola et al [24], 6De Sousa et al [30])

Effectiveness of embryo transfer in cows - risk factors including in vivo derived and in vitro produced embryos

Pubblicato online: 27 set 2021

Pagine: 123 - 131

Ricevuto: 13 lug 2021

Accettato: 28 ago 2021

DOI: https://doi.org/10.2478/acb-2021-0017

Parole chiaveembryo transfer, cattle reproduction, assisted reproduction techniques

© 2021 Maria Wieczorkiewicz et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Influence of factors related to embryo transfer on the conception rates ((own compilation based on: 1Ledgard et al. [43], 2Roper et al. [16], 3Jaśkowski JM et al. [49] 3Del Campo et al. [13], 4Jaśkowski BM et al. [46]

Effects of factors related to in vivo or in vitro embryo production on conception rates (own compilation based on: 1Pontes et al [5], 2Ferraz et al [6], 3Donaldson et al [21], 4Arreseigor et al [20], 5Mikola et al [24], 6De Sousa et al [30])

Parole chiave
embryo transfer, cattle reproduction, assisted reproduction techniques