Comparison of microfluidic and swim-up sperm separation methods for IVF

Figure 1

Demonstration of efficiency of the separation methods_ Ability of the separation methods to reduce the number of spermatozoa with fragmented DNA was evaluated, the initial DFI value of the unprocessed ejaculate is considered 100%

PATIENT	DFI (%; RELATIVE VALUE)			DIFFERENCES BETWEEN SWIM-UP AND FERTILE
	SEMEN	SWIM-UP	FERTILE
1	100	55.0	50.7	4.3
2	100	68.0	66.0	2.0
3	100	42.8	26.2	16.0
4	100	69.0	41.3	27.7
5	100	81.0	62.5	18.5
6	100	15.4	6.3	9.1
7	100	55.9	46.8	9.1
8	100	28.1	21.3	6.8
9	100	32.6	25.5	7.1
10	100	37.5	33.7	3.8
Ø	100	48.5	38.0	10.5

DFI values of all patients before and after separation by swim-up and FERTILE method

PATIENT	DFI (%; ABSOLUTE VALUE)			DIFFERENCES BETWEEN SWIM-UP AND FERTILE
	SEMEN	SWIM-UP	FERTILE
1	13.0	7.2	6.6	0.6
2	9.4	6.4	6.2	0.2
3	21.0	9.0	5.5	3.5
4	23.0	16.0	9.5	6.5
5	16.0	13.0	10.0	3.0
6	11.0	1.4	0.7	0.7
7	22.0	12.3	10.3	2.0
8	16.0	4.5	3.4	1.1
9	43.0	14.0	11.0	3.0
10	8.0	3.0	2.7	0.3
Ø	18.2	8.7	6.6	2.1

List of all patients

PATIENT	AGE	CONCENTRATION (MIL/ML)	MOTILITY (%)	NORMAL MORPHOLOGY (%)	DIAGNOSIS
1	27	42	62	11	Normozoospermia
2	30	27	43	6	Normozoospermia
3	38	37	54	12	Normozoospermia
4	29	45	64	15	Normozoospermia
5	32	49	45	13	Normozoospermia
6	33	104	61	12	Normozoospermia
7	37	34	28	7	Asthenozoospermia
8	40	78	67	11	Normozoospermia
9	35	56	39	5	Asthenozoospermia
10	27	38	48	9	Normozoospermia