<abstract xmlns="http://www.w3.org/1999/xhtml">

Despite the significant gains made in cancer therapy, cancer remains a major cause of global deaths due to rapid drug resistance. Therefore, urgent concerted efforts towards the discovery and development of newer and effective anticancer agents cannot be overemphasized. This study investigated <italic>in vitro</italic> cytotoxicity potential of methanol extracts of the root, stem, and leaves of <italic>Mezoneuron benthamianum</italic>. Leaf, stem and root samples were collected, authenticated, dried, separately pulverized and extracted in methanol. The methanol extracts were analysed for the presence of phytochemicals and cytotoxic potential evaluated by tetrazolium 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay on selected human cancer cells lines, HeLa (cervical cancer) and HEp-2 (epidermal carcinoma of the larynx), using mammalian Vero cells as a negative control. Data generated was subjected to descriptive statistics. The flavonoids in the plant was between 40 - 67.2% and was significantly higher (<italic>p</italic> &lt; 0.05) compared to alkaloids and saponins. At concentrations of ≥ 50 µg/mL, the extracts exhibited 100% cytotoxicity on the cancer cells. The methanol root and leaf extracts with CC50 of 15.64 and 11.38 µg/mL were more cytotoxic on HeLa and HEp-2, respectively. In comparison to the stem and root extracts, the methanol leaf extract was selectively more toxic to cancer cell lines than Vero cells (CC50 = 28.89 µg/mL). Preliminary investigation reveals that <italic>Mezoneuron benthamianum</italic> contain bioactive compounds that possess promising anticancer potential that could be exploited.
</abstract>

Despite the significant gains made in cancer therapy, cancer remains a major cause of global deaths due to rapid drug resistance. Therefore, urgent concerted efforts towards the discovery and development of newer and effective anticancer agents cannot be overemphasized. This study investigated in vitro cytotoxicity potential of methanol extracts of the root, stem, and leaves of Mezoneuron benthamianum. Leaf, stem and root samples were collected, authenticated, dried, separately pulverized and extracted in methanol. The methanol extracts were analysed for the presence of phytochemicals and cytotoxic potential evaluated by tetrazolium 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay on selected human cancer cells lines, HeLa (cervical cancer) and HEp-2 (epidermal carcinoma of the larynx), using mammalian Vero cells as a negative control. Data generated was subjected to descriptive statistics. The flavonoids in the plant was between 40 - 67.2% and was significantly higher (p < 0.05) compared to alkaloids and saponins. At concentrations of ≥ 50 µg/mL, the extracts exhibited 100% cytotoxicity on the cancer cells. The methanol root and leaf extracts with CC50 of 15.64 and 11.38 µg/mL were more cytotoxic on HeLa and HEp-2, respectively. In comparison to the stem and root extracts, the methanol leaf extract was selectively more toxic to cancer cell lines than Vero cells (CC50 = 28.89 µg/mL). Preliminary investigation reveals that Mezoneuron benthamianum contain bioactive compounds that possess promising anticancer potential that could be exploited.

Phytochemical Screening and <italic>In-Vitro</italic> Cytotoxic Activities of Methanolic Extracts of <italic>Mezoneuron benthamianum</italic> Baill. Against Cancer Cells Lines

Department of Pharmaceutical Microbiology

Department of Pharmacology and Therapeutics, College of Health Sciences

Department of Pharmacology and Therapeutics, College of Medicine

Acta Biologica Marisiensis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Phytochemical Screening and  Cytotoxic Activities of Methanolic Extracts of  Baill. Against Cancer Cells Lines

Despite the significant gains made in cancer therapy, cancer remains a major cause of global deaths due to rapid drug resistance. Therefore, urgent concerted...

Phytochemical Screening and In-Vitro Cytotoxic Activities of Methanolic Extracts of Mezoneuron benthamianum Baill. Against Cancer Cells Lines

Pubblicato online: 23 giu 2023

Pagine: 62 - 72

Ricevuto: 06 feb 2023

Accettato: 16 giu 2023

DOI: https://doi.org/10.2478/abmj-2023-0006

Parole chiave
cancer cells, chemotherapy, MTT

© 2023 Bolaji B. Oluremi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Phytochemical Screening and In-Vitro Cytotoxic Activities of Methanolic Extracts of Mezoneuron benthamianum Baill. Against Cancer Cells Lines

Pubblicato online: 23 giu 2023

Pagine: 62 - 72

Ricevuto: 06 feb 2023

Accettato: 16 giu 2023

DOI: https://doi.org/10.2478/abmj-2023-0006

Parole chiavecancer cells, chemotherapy, MTT

© 2023 Bolaji B. Oluremi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Parole chiave
cancer cells, chemotherapy, MTT