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Antibody response to enterotoxigenic Bacteroides fragilis of Filipino colorectal cancer patients

Ana Maria D. Cariño
Ana Maria D. Cariño
, 
Gregg Austine Balanag
Gregg Austine Balanag
, 
Edrienne Myenna Magat
Edrienne Myenna Magat
, 
Allan Fellizar
Allan Fellizar
, 
Teresa Sy Ortin
Teresa Sy Ortin
, 
Oliver Villaflores
Oliver Villaflores
, 
Leonardo Guevarra
Leonardo Guevarra
 e 
Pia Marie Albano
Pia Marie Albano
   | 28 dic 2023
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Article Category: Brief communication
Pubblicato online: 28 dic 2023
Pagine: 273 - 280
DOI: https://doi.org/10.2478/abm-2023-0070
Parole chiave
© 2023 Ana Maria D. Cariño et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1.

An overview of the method adopted in this study. CRC, colorectal cancer; ELISA, enzyme-linked immunosorbent assay; ETBF, enterotoxigenic Bacteroides fragilis.
An overview of the method adopted in this study. CRC, colorectal cancer; ELISA, enzyme-linked immunosorbent assay; ETBF, enterotoxigenic Bacteroides fragilis.

Figure 2.

Checkerboard titration results at 1:1,000 secondary antibody dilution: X-axis = primary antibody (pooled plasma samples) concentration; Y-axis = anti-ETBF IgG antibody level absorbance at OD450 nm. (A) 80 μg/mL coating antigen; (B) 50 μg/mL coating antigen; (C) 25 μg/mL coating antigen; (D) 10 μg/mL coating antigen; (E) 5 μg/mL coating antigen. ETBF, enterotoxigenic Bacteroides fragilis.
Checkerboard titration results at 1:1,000 secondary antibody dilution: X-axis = primary antibody (pooled plasma samples) concentration; Y-axis = anti-ETBF IgG antibody level absorbance at OD450 nm. (A) 80 μg/mL coating antigen; (B) 50 μg/mL coating antigen; (C) 25 μg/mL coating antigen; (D) 10 μg/mL coating antigen; (E) 5 μg/mL coating antigen. ETBF, enterotoxigenic Bacteroides fragilis.

Figure 3.

Comparison of optical density (OD) values (nanometer) of anti-ETBF IgG and anti-ETBF IgA levels between CRC patients and healthy individuals (cases, n = 39; controls, n = 39). (A) Bacterial lysate as coating antigen; (B) culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.
Comparison of optical density (OD) values (nanometer) of anti-ETBF IgG and anti-ETBF IgA levels between CRC patients and healthy individuals (cases, n = 39; controls, n = 39). (A) Bacterial lysate as coating antigen; (B) culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.

Figure 4.

Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by (A) tumor grade and (B) tumor stage using bacterial lysate as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.
Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by (A) tumor grade and (B) tumor stage using bacterial lysate as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.

Figure 5.

Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by tumor grade (A) and tumor stage (B) using bacterial culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.
Comparison of the mean OD values (nanometer) of the anti-ETBF IgG and IgA antibody levels of CRC patients when grouped by tumor grade (A) and tumor stage (B) using bacterial culture broth as coating antigen. CRC, colorectal cancer; ETBF, enterotoxigenic Bacteroides fragilis.

Demographic and clinical characteristics of CRC cases and controls

Characteristics Cases (n = 39) Controls (n = 39) P value*
Sex
  Male 22 (56%) 22 (56%)
  Female 17 (44%) 17 (44%)
Age at initial diagnosis (years)
  <50 11 (28%) 10 (26%) 0.49
  ≥50 28 (72%) 29 (74%) 0.43
Mean age at initial diagnosis (years ± SD) 58 ± 12
Median age at initial diagnosis 61
Tumor grade
  G1 (well differentiated) 11 (28%)
  G2 & G3 (moderately and poorly differentiated) 17 (44%)
  No information available 11 (28%)
Tumor stage
  T1 & T2 (early) 8 (20%)
  T3 & T4 (advanced) 21 (54%)
  No information available 10 (26%)
eISSN:
1875-855X
Lingua:
Inglese
Frequenza di pubblicazione:
6 volte all'anno
Argomenti della rivista:
Medicine, Assistive Professions, Nursing, Basic Medical Science, other, Clinical Medicine
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