miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A
Categoria dell'articolo: Original article
Pubblicato online: 09 ott 2023
Pagine: 124 - 135
DOI: https://doi.org/10.2478/abm-2023-0052
Parole chiave
© 2023 Xiaoyong Huang et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
The ambiguity of renal cell carcinoma (RCC) symptoms hinders early diagnosis, thereby contributing to high mortality rates. By attaching to the 3′-untranslated region (UTR) of the target gene, microRNAs (miRNAs) exert significant control over the expression of genes.
Objectives
To investigate the influence of miR-30c-2-3p and DNA topoisomerase II alpha (TOP2A) on RCC growth and the mechanisms underlying the regulation of its expression.
Methods
The expression of miRNA-30c-2-3p and
Results
miR-30c-2-p is underexpressed in RCC cells. Overexpression of miR-30c-2-p promotes apoptosis and inhibits proliferation of ACHN, Caki-1, and 786-O cells. miR-30c-2-3p targets TOP2A, which is elevated in RCC tissues and cells, whereas TOP2A silencing inhibits the proliferation ability of RCC cells. The miRNA-30c-2-3p inhibitor compromises TOP2A shRNA-induced apoptosis of RCC. RCC cells cotransfected with miRNA-30c-2-3p inhibitors and TOP2A shRNAs have a higher proliferation rate than those transfected with only TOP2A shRNAs.
Conclusions
Collectively, our results verify that miRNA-30c-2-3p has a tumor suppressor property. miRNA-30c-2-3p inhibits the proliferation of RCC through regulation of TOP2A. The data provide a viable therapeutic target for RCC.