<abstract xmlns="http://www.w3.org/1999/xhtml"> Glycopolymers of two types were isolated from the cell wall of Lactococcus lactis BIM B-1024 by stepwise extraction with cold and hot 10% CCl3CO2H and separated by anion-exchange gel chromatography. The following structures of the glycopolymers were established by sugar analysis, dephosphorylation with 48% HF, 1D and 2D NMR spectroscopy, and ESI-MS: β-D-Galp-(1→4)-β-D-Glcp-(1→6)┐→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp-(1→ PSIβ-D-Glcp-(1→3)┐→60-α-D-GalpNAc-(1→3)-β-D-GalpNAc-(1→5)-Rib-ol-1-P-(O→ PSIIβ-D-Glcp-(1→4)┘Polysaccharides with the same or similar structures to PSI have been found earlier in various Lactobacillus species, whereas, to our knowledge, the structure of PSII is new.</abstract>

Glycopolymers of two types were isolated from the cell wall of Lactococcus lactis BIM B-1024 by stepwise extraction with cold and hot 10% CCl3CO2H and separated by anion-exchange gel chromatography. The following structures of the glycopolymers were established by sugar analysis, dephosphorylation with 48% HF, 1D and 2D NMR spectroscopy, and ESI-MS: β-D-Galp-(1→4)-β-D-Glcp-(1→6)┐→4)-β-D-Galp-(1→4)-β-D-Glcp-(1→4)-α-D-Glcp-(1→ PSIβ-D-Glcp-(1→3)┐→60-α-D-GalpNAc-(1→3)-β-D-GalpNAc-(1→5)-Rib-ol-1-P-(O→ PSIIβ-D-Glcp-(1→4)┘Polysaccharides with the same or similar structures to PSI have been found earlier in various Lactobacillus species, whereas, to our knowledge, the structure of PSII is new.

Structures of cell-wall glycopolymers of Lactococcus lactis BIM B-1024

The EuroBiotech Journal

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Structures of cell-wall glycopolymers of Lactococcus lactis BIM B-1024"}

Glycopolymers of two types were isolated from the cell wall of Lactococcus lactis BIM B-1024 by stepwise extraction with cold and hot 10% CCl3CO2H and...

Structures of cell-wall glycopolymers of Lactococcus lactis BIM B-1024

Pubblicato online: 27 gen 2017

Pagine: 41 - 45

Ricevuto: 27 gen 2017

DOI: https://doi.org/10.24190/ISSN2564-615X/2017/01.07

© 2018

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.