<abstract xmlns="http://www.w3.org/1999/xhtml"><p>This work presents results of the research on the occurrence of <italic>Coxiella burnetii</italic> and <italic>Francisella tularensis</italic> in the tissues of wild-living animals and ticks collected from Drawsko County, West Pomeranian Voivodeship. The real-time PCR testing for the pathogens comprised 928 samples of animal internal organs and 1551 ticks. The presence of <italic>C. burnetii</italic> was detected in 3% of wild-living animals and in 0.45–3.45% (dependent on collection areas) of ticks. The genetic sequences of <italic>F. tularensis</italic> were present in 0.49 % of ticks (only in one location – Drawa) and were not detected in animal tissues. The results indicate respectively low proportion of animals and ticks infected with <italic>C. burnetii</italic> and <italic>F. tularensis</italic>.</p></abstract>

This work presents results of the research on the occurrence of Coxiella burnetii and Francisella tularensis in the tissues of wild-living animals and ticks collected from Drawsko County, West Pomeranian Voivodeship. The real-time PCR testing for the pathogens comprised 928 samples of animal internal organs and 1551 ticks. The presence of C. burnetii was detected in 3% of wild-living animals and in 0.45–3.45% (dependent on collection areas) of ticks. The genetic sequences of F. tularensis were present in 0.49 % of ticks (only in one location – Drawa) and were not detected in animal tissues. The results indicate respectively low proportion of animals and ticks infected with C. burnetii and F. tularensis.

Detection of <italic>Coxiella burnetii</italic> and <italic>Francisella tularensis</italic> in Tissues of Wild-living Animals and in Ticks of North-west Poland

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Detection of  and  in Tissues of Wild-living Animals and in Ticks of North-west Poland

This work presents results of the research on the occurrence of Coxiella burnetii and Francisella tularensis in the tissues of wild-living animals and ticks...

Site	Number of pools	PoolSize	Number of positive pools for C. burnetii	Number of positive pools for F. tularensis	Number of individuals	Total number of individuals	Estimated prevalence (%)¹C. burnetii	Estimated prevalence (%)¹F. tularensis
Drawa	9	20	1	0	59	211	0.5%	0.49%
	1	19	0	0	52
	1	12	0	1	100
Karwice	23	20	2	0	460	460	0.45%	0
Lake Konotop	17	20	8	0	340	377	3.1%	0
	1	19	0	0	19
	1	13	0	0	13
	1	5	1	0	5
Konotop	5	20	2	0	100	117	3.45%	0
Konotop	1	17	1	0	17	117	3.45%	0
Oleszno	18	20	2	0	360	386	0.55%	0
	1	19	0	0	19
	1	7	0	0	7

	C. burnetii	F. tularensis
	IS1111⁺ (Klee et al. 2006)	fopA (Emmanuel et al. 2003)	tul4 (Fujita et al. 2006)
Forward primer	5’-GTCTTAAGGTGGGCTGCGT G-3’	5’-AACAATGGCACCTAGTAAT ATTTCTGG-3’	5’-ATTACAATGGCAGGCTCC AGA-3’
Reverse primer	5’-CCCCGAATCTCATTGATC AGC-3’	5’-CCACCAAAGAACCATGTT AAACC-3’	5’-TGCCCAAGTTTTATCGTTC TTCT-3’
Probe	5’-FAM-AGCGAACCATTGGTATC GGACGTTT-TAMRA-TATGG-Pho-3’	5’-FAM-TGGCAGAGCGGGTACT AACATGATTGGT-TAMRA-3’	5’-FAM TCCTAAGTGCCATGAT ACAAGCTTCCCAATTACTAAG-BHQ1-3’

Detection of Coxiella burnetii and Francisella tularensis in Tissues of Wild-living Animals and in Ticks of North-west Poland

Article Category: short-communication

Pubblicato online: 10 dic 2018

Pagine: 529 - 534

Ricevuto: 12 lug 2018

Accettato: 29 set 2018

DOI: https://doi.org/10.21307/pjm-2018-059

Parole chiave
reservoirs, real-time PCR

© 2018 Agata Bielawska-Drózd et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The estimated prevalence of positive ticks pools for C. burnetii and F. tularensis.

The oligonucleotides used in real-time PCR.

Detection of Coxiella burnetii and Francisella tularensis in Tissues of Wild-living Animals and in Ticks of North-west Poland

Article Category: short-communication

Pubblicato online: 10 dic 2018

Pagine: 529 - 534

Ricevuto: 12 lug 2018

Accettato: 29 set 2018

DOI: https://doi.org/10.21307/pjm-2018-059

Parole chiavereservoirs, real-time PCR

© 2018 Agata Bielawska-Drózd et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

The estimated prevalence of positive ticks pools for C. burnetii and F. tularensis.

The oligonucleotides used in real-time PCR.

Parole chiave
reservoirs, real-time PCR