Variable | Level | ||||
---|---|---|---|---|---|
–2 | –1 | 0 | 1 | 2 | |
A: Post induction temperature (T) (°C) | 20 | 24.25 | 28.5 | 32.75 | 37 |
B: Dissolved oxygen (DO) (%) | 10 | 20 | 30 | 40 | 50 |
Run | Post induction Temperature (T) (°C) | Dissolved Oxygen (DO) (%) | Response 1 Recombinant Protein Expression Level (%) | Response 2 Cell Growth (Abs 600nm) | ||
---|---|---|---|---|---|---|
1 | –1 | 24.25 | –1 | 20 | 8.0 ± 0.9 | 1.83 ± 0.18 |
2 | +1 | 32.75 | –1 | 20 | 15.3 ± 1.2 | 2.60 ± 0.19 |
3 | –1 | 24.25 | +1 | 40 | 10.1 ± 1.0 | 1.02 ± 0.11 |
4 | +1 | 32.75 | +1 | 40 | 8.4 ± 0.8 | 5.60 ± 0.29 |
5 | –2 | 20.0 | 0 | 30 | 4.6 ± 0.4 | 1.36 ± 0.11 |
6 | +2 | 37.0 | 0 | 30 | 11.1 ± 1.1 | 3.40 ± 0.22 |
7 | 0 | 28.5 | –2 | 10 | 10.0 ± 0.9 | 1.87 ± 0.21 |
8 | 0 | 28.5 | +2 | 50 | 3.8 ± 0.4 | 2.05 ± 0.20 |
9 | 0 | 28.5 | 0 | 30 | 14.6 ± 1.2 | 2.45 ± 0.19 |
10 | 0 | 28.5 | 0 | 30 | 14.2 ± 1.2 | 2.60 ± 0.19 |
Source of variation | Degree of freedom | Sum of squares | Mean squares | |||
---|---|---|---|---|---|---|
Recombinant Protein Expression Level (%) | Model | 5 | 141.50 | 28.30 | 120.36 | 0.0002 |
A-Temperature (°C) | 1 | 29.14 | 29.14 | 123.94 | 0.0004 | |
B-Dissolved Oxygen (%) | 1 | 24.65 | 24.65 | 104.85 | 0.0005 | |
AB | 1 | 20.25 | 20.25 | 86.13 | 0.0007 | |
A2 | 1 | 42.94 | 42.94 | 182.62 | 0.0002 | |
B2 | 1 | 56.17 | 56.17 | 238.90 | 0.0001 | |
Error | 1 | 0.058 | 0.058 | – | – | |
Total | 9 | 142.44 | – | – | – | |
Cell Growth (Abs 600 nm) | Model | 3 | 11.58 | 3.86 | 6.82 | 0.0232 |
A-Temperature (°C) | 1 | 7.41 | 7.41 | 13.10 | 0.0111 | |
B-Dissolved Oxygen (%) | 1 | 0.54 | 0.54 | 0.96 | 0.3655 | |
AB | 1 | 3.63 | 3.63 | 6.41 | 0.0445 | |
Error | 1 | 0.011 | 0.011 | – | – | |
Total | 9 | 14.98 | – | – | – |