Transcriptome Analyses of Pre-parasitic and Parasitic Meloidogyne Chitwoodi Race 1 to Identify Putative Effector Genes

Almagro Armenteros, J. J., Tsirigos, K. D., Sønderby, C. K., Petersen, T. N., Winther, O., Brunak, S., von Heijne, G. and Nielsen, H. 2019. SignalP 5.0 improves signal peptide predictions using deep neural networks. Nature Biotechnology 37:420–423. Almagro Armenteros J. J. Tsirigos K. D. Sønderby C. K. Petersen T. N. Winther O. Brunak S. von Heijne G. and Nielsen H. 2019 . SignalP 5.0 improves signal peptide predictions using deep neural networks . Nature Biotechnology 37 : 420 – 423 . 10.1038/s41587-019-0036-z30778233 Search in Google Scholar

Bali, S., Hu, S., Vining, K., Brown, C. R., Majtahedi, H., Zhang, L., Gleason, C. and Sathuvalli, V. 2021. Nematode Genome Announcement: Draft genome of Meloidogyne chitwoodi, an economically important pest of potato in the Pacific Northwest. Molecular Plant-Microbe Interactions March 29, doi: 10.1094/MPMI-12-20-0337-A. Bali S. Hu S. Vining K. Brown C. R. Majtahedi H. Zhang L. Gleason C. and Sathuvalli V. 2021 . Nematode Genome Announcement: Draft genome of Meloidogyne chitwoodi, an economically important pest of potato in the Pacific Northwest . Molecular Plant-Microbe Interactions March 29 , doi: 10.1094/MPMI-12-20-0337-A . 33779267 Open DOISearch in Google Scholar

Bali, S., Vining, K., Gleason, C., Majtahedi, H., Brown, C. R. and Sathuvalli, V. 2019. Transcriptome profiling of resistance response to Meloidogyne chitwoodi introgressed from wild species Solanum bulbocastanum into cultivated potato. BMC Genomics 20:907. Bali S. Vining K. Gleason C. Majtahedi H. Brown C. R. and Sathuvalli V. 2019 . Transcriptome profiling of resistance response to Meloidogyne chitwoodi introgressed from wild species Solanum bulbocastanum into cultivated potato . BMC Genomics 20 : 907 . 10.1186/s12864-019-6257-1688358231779600 Search in Google Scholar

Barcala, M., García, A., Cabrera, J., Casson, S., Lindsey, K., Favery, B., García-Casado, G., Solano, R., Fenoll, C. and Escobar, C. 2010. Early transcriptomic events in microdissected Arabidopsis nematode-induced giant cells. The Plant Journal 61:698–712. Barcala M. García A. Cabrera J. Casson S. Lindsey K. Favery B. García-Casado G. Solano R. Fenoll C. and Escobar C. 2010 . Early transcriptomic events in microdissected Arabidopsis nematode-induced giant cells . The Plant Journal 61 : 698 – 712 . 10.1111/j.1365-313X.2009.04098.x20003167 Search in Google Scholar

Bert, W., Leliaert, F., Vierstraete, A. R., Vanfleteren, J. R. and Borgonie, G. 2008. Molecular phylogeny of the Tylenchina and evolution of the female gonoduct (Nematoda: Rhabditida). Molecular Phylogenetics and Evolution 48:728–744. Bert W. Leliaert F. Vierstraete A. R. Vanfleteren J. R. and Borgonie G. 2008 . Molecular phylogeny of the Tylenchina and evolution of the female gonoduct (Nematoda: Rhabditida) . Molecular Phylogenetics and Evolution 48 : 728 – 744 . 10.1016/j.ympev.2008.04.01118502668 Search in Google Scholar

Birch, P. R. J., Bryan, G., Fenton, B., Gilroy, E. M., Hein, I., Jones, J. T., Prashar, A., Taylor, M. A., Torrance, L. and Toth, I. K. 2012. Crops that feed the world 8: Potato: are the trends of increased global production sustainable? Food Security 4:477–508. Birch P. R. J. Bryan G. Fenton B. Gilroy E. M. Hein I. Jones J. T. Prashar A. Taylor M. A. Torrance L. and Toth I. K. 2012 . Crops that feed the world 8: Potato: are the trends of increased global production sustainable? Food Security 4 : 477 – 508 . 10.1007/s12571-012-0220-1 Search in Google Scholar

Bird, A. F. 1961. The ultrastructure and histochemistry of a nematode-induced giant cell. The Journal of Biophysical and Biochemical Cytology 11:701–715. Bird A. F. 1961 . The ultrastructure and histochemistry of a nematode-induced giant cell . The Journal of Biophysical and Biochemical Cytology 11 : 701 – 715 . 10.1083/jcb.11.3.701222512613869341 Search in Google Scholar

Bolger, A. M., Lohse, M. and Usadel, B. 2014. Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120. Bolger A. M. Lohse M. and Usadel B. 2014 . Trimmomatic: a flexible trimmer for Illumina sequence data . Bioinformatics 30 : 2114 – 2120 . 10.1093/bioinformatics/btu170410359024695404 Search in Google Scholar

Bybd, D. W., Kirkpatrick, T. and Barker, K. R. 1983. An improved technique for clearing and staining plant tissues for detection of nematodes. Journal of Nematology 15:142–143. Bybd D. W. Kirkpatrick T. and Barker K. R. 1983 . An improved technique for clearing and staining plant tissues for detection of nematodes . Journal of Nematology 15 : 142 – 143 . Search in Google Scholar

CABI/EPPO. 2012. Meloidogyne chitwoodi. [Distribution map]. Distribution Maps of Plant Diseases, No. April, Wallingford: CABI, Map 803 (Edition 2). CABI/EPPO . 2012 . Meloidogyne chitwoodi . [Distribution map]. Distribution Maps of Plant Diseases, No. April , Wallingford : CABI , Map 803 (Edition 2) . Search in Google Scholar

Caillaud, M.- C., Dubreuil, G., Quentin, M., Perfus-Barbeoch, L., Lecomte, P., de Almeida Engler, J., Abad, P., Rosso, M.- N. and Favery, B. 2008. Root-knot nematodes manipulate plant cell functions during a compatible interaction. Journal of Plant Physiology 165:104–113. Caillaud M.- C. Dubreuil G. Quentin M. Perfus-Barbeoch L. Lecomte P. de Almeida Engler J. Abad P. Rosso M.- N. and Favery B. 2008 . Root-knot nematodes manipulate plant cell functions during a compatible interaction . Journal of Plant Physiology 165 : 104 – 113 . 10.1016/j.jplph.2007.05.00717681399 Search in Google Scholar

Dubreuil, G., Magliano, M., Deleury, E., Abad, P. and Rosso, M. N. 2007. Transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism. New Phytologist 176:426–436. Dubreuil G. Magliano M. Deleury E. Abad P. and Rosso M. N. 2007 . Transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism . New Phytologist 176 : 426 – 436 . 10.1111/j.1469-8137.2007.02181.x17692078 Search in Google Scholar

Elashry, A. M., Habash, S. S., Vijayapalani, P., Brocke-Ahmadinejad, N., Blümel, R., Seetharam, A., Schoof, H. and Grundler, F. M. W. 2020. Transcriptome and parasitome analysis of beet cyst nematode Heterodera schachtii. Scientific Reports 10:3315. Elashry A. M. Habash S. S. Vijayapalani P. Brocke-Ahmadinejad N. Blümel R. Seetharam A. Schoof H. and Grundler F. M. W. 2020 . Transcriptome and parasitome analysis of beet cyst nematode Heterodera schachtii . Scientific Reports 10 : 3315 . 10.1038/s41598-020-60186-0703998532094373 Search in Google Scholar

Finley, A. M. 1981. Histopathology of Meloidogyne chitwoodi (Golden et al.) on Russett Burbank Potato. Journal of Nematology 13:486–491. Finley A. M. 1981 . Histopathology of Meloidogyne chitwoodi (Golden et al.) on Russett Burbank Potato . Journal of Nematology 13 : 486 – 491 . Search in Google Scholar

Golden, A. M., Obannon, J. H., Santo, G. S. and Finley, A. M. 1980. Description and some observations of Meloidogyne chitwoodi n. sp. (Meloidogynidae), a root-knot nematode on potato in the Pacific Northwest. Journal of Nematology 12:319–327. Golden A. M. Obannon J. H. Santo G. S. and Finley A. M. 1980 . Description and some observations of Meloidogyne chitwoodi n. sp. (Meloidogynidae), a root-knot nematode on potato in the Pacific Northwest . Journal of Nematology 12 : 319 – 327 . Search in Google Scholar

Haas, B. J., Papanicolaou, A., Yassour, M., Grabherr, M., Blood, P. D., Bowden, J., Couger, M. B., Eccles, D., Li, B., Lieber, M., MacManes, M. D., Ott, M., Orvis, J., Pochet, N., Strozzi, F., Weeks, N., Westerman, R., William, T., Dewey, C. N., Henschel, R., LeDuc, R. D., Friedman, N. and Regev, A. 2013. De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nature Protocols 8:1494–1512. Haas B. J. Papanicolaou A. Yassour M. Grabherr M. Blood P. D. Bowden J. Couger M. B. Eccles D. Li B. Lieber M. MacManes M. D. Ott M. Orvis J. Pochet N. Strozzi F. Weeks N. Westerman R. William T. Dewey C. N. Henschel R. LeDuc R. D. Friedman N. and Regev A. 2013 . De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis . Nature Protocols 8 : 1494 – 1512 . 10.1038/nprot.2013.084387513223845962 Search in Google Scholar

Huang, G., Dong, R., Allen, R., Davis, E. L., Baum, T. J. and Hussey, R. S. 2005. Two chorismate mutase genes from the root-knot nematode Meloidogyne incognita. Molecular Plant Pathology 6:23–30. Huang G. Dong R. Allen R. Davis E. L. Baum T. J. and Hussey R. S. 2005 . Two chorismate mutase genes from the root-knot nematode Meloidogyne incognita . Molecular Plant Pathology 6 : 23 – 30 . 10.1111/j.1364-3703.2004.00257.x20565635 Search in Google Scholar

Huang, G., Gao, B., Maier, T., Allen, R., Davis, E. L., Baum, T. J. and Hussey, R. S. 2003. A profile of putative parasitism genes expressed in the esophageal gland cells of the root-knot nematode Meloidogyne incognita. Molecular Plant–Microbe Interactions 16:376–381. Huang G. Gao B. Maier T. Allen R. Davis E. L. Baum T. J. and Hussey R. S. 2003 . A profile of putative parasitism genes expressed in the esophageal gland cells of the root-knot nematode Meloidogyne incognita . Molecular Plant–Microbe Interactions 16 : 376 – 381 . 10.1094/MPMI.2003.16.5.37612744507 Search in Google Scholar

Ingham, R. E., Hamm, P. B., Williams, R. E. and Swanson, W. H. 2000. Control of Meloidogyne chitwoodi in potato with fumigant and nonfumigant nematicides. Journal of Nematology 32:556–565. Ingham R. E. Hamm P. B. Williams R. E. and Swanson W. H. 2000 . Control of Meloidogyne chitwoodi in potato with fumigant and nonfumigant nematicides . Journal of Nematology 32 : 556 – 565 . Search in Google Scholar

Ingham, R. E., Hamm, P. B., Baune, M., David, N. L. and Wade, N. M. 2007. Control of Meloidogyne chitwoodi in potato with shank-injected metam sodium and other nematicides. Journal of Nematology 39:161–168. Ingham R. E. Hamm P. B. Baune M. David N. L. and Wade N. M. 2007 . Control of Meloidogyne chitwoodi in potato with shank-injected metam sodium and other nematicides . Journal of Nematology 39 : 161 – 168 . Search in Google Scholar

Jacob, J. J. and van Bezooijen, J. 1984. Manual for Practical Work in Nematology. LH. Search in Google Scholar

King, B. A. and Taberna, J. P. Jr 2013. Site-specific management of Meloidogyne chitwoodi in Idaho potatoes using 1,3-Dichloropropene; approach, experiences, and economics. Journal of Nematology 45:202–213. King B. A. and Taberna J. P. Jr 2013 . Site-specific management of Meloidogyne chitwoodi in Idaho potatoes using 1,3-Dichloropropene; approach, experiences, and economics . Journal of Nematology 45 : 202 – 213 . Search in Google Scholar

Kooliyottil, R., Dandurand, L.- M., Kuhl, J. C., Caplan, A., Xiao, F., Mimee, B. and Lafond-Lapalme, J. 2019. Transcriptome analysis of Globodera pallida from the susceptible host Solanum tuberosum or the resistant plant Solanum sisymbriifolium. Scientific Reports 9:13256. Kooliyottil R. Dandurand L.- M. Kuhl J. C. Caplan A. Xiao F. Mimee B. and Lafond-Lapalme J. 2019 . Transcriptome analysis of Globodera pallida from the susceptible host Solanum tuberosum or the resistant plant Solanum sisymbriifolium . Scientific Reports 9 : 13256 . 10.1038/s41598-019-49725-6674440831519937 Search in Google Scholar

Koutsovoulos, G. D., Poullet, M., Elashry, A., Kozlowski, D. K. L., Sallet, E., Da Rocha, M., Perfus-Barbeoch, L., Martin-Jimenez, C., Frey, J. E., Ahrens, C. H., Kiewnick, S. and Danchin, E. G. J. 2020. Genome assembly and annotation of Meloidogyne enterolobii, an emerging parthenogenetic root-knot nematode. Scientific Data 7:324. Koutsovoulos G. D. Poullet M. Elashry A. Kozlowski D. K. L. Sallet E. Da Rocha M. Perfus-Barbeoch L. Martin-Jimenez C. Frey J. E. Ahrens C. H. Kiewnick S. and Danchin E. G. J. 2020 . Genome assembly and annotation of Meloidogyne enterolobii, an emerging parthenogenetic root-knot nematode . Scientific Data 7 : 324 . 10.1038/s41597-020-00666-0753618533020495 Search in Google Scholar

Krogh, A., Larsson, B., von Heijne, G. and Sonnhammer, E. L. 2001. Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. Journal of Molecular Biology 305:567–580. Krogh A. Larsson B. von Heijne G. and Sonnhammer E. L. 2001 . Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes . Journal of Molecular Biology 305 : 567 – 580 . 10.1006/jmbi.2000.431511152613 Search in Google Scholar

Lambert, K. N., Allen, K. D. and Sussex, I. M. 1999. Cloning and characterization of an esophageal-gland-specific chorismate mutase from the phytoparasitic nematode Meloidogyne javanica. Molecular Plant–Microbe Interactions 12:328–336. Lambert K. N. Allen K. D. and Sussex I. M. 1999 . Cloning and characterization of an esophageal-gland-specific chorismate mutase from the phytoparasitic nematode Meloidogyne javanica . Molecular Plant–Microbe Interactions 12 : 328 – 336 . 10.1094/MPMI.1999.12.4.32810188271 Search in Google Scholar

Lee, I. H., Shim, D., Jeong, J. C., Sung, Y. W., Nam, K. J., Yang, J.- W., Ha, J., Lee, J. J. and Kim, Y.- H. 2019. Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-resistant and susceptible sweetpotato cultivars. Planta 249:431–444. Lee I. H. Shim D. Jeong J. C. Sung Y. W. Nam K. J. Yang J.- W. Ha J. Lee J. J. and Kim Y.- H. 2019 . Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-resistant and susceptible sweetpotato cultivars . Planta 249 : 431 – 444 . 10.1007/s00425-018-3001-z30232599 Search in Google Scholar

Lima, F. S. O., Mattos, V. S., Silva, E. S., Carvalho, M. A. S., Teixeira, R. A., Silva, J. C. and Correa, V. R. 2018. “Nematodes affecting potato and sustainable practices for their Management”, In Yildiz, M. (Ed.), Potato – From Incas to All Over the World, IntechOpen, Available at: https://www.intechopen.com/chapters/59075. Lima F. S. O. Mattos V. S. Silva E. S. Carvalho M. A. S. Teixeira R. A. Silva J. C. and Correa V. R. 2018 . “Nematodes affecting potato and sustainable practices for their Management” , In Yildiz M. (Ed.), Potato – From Incas to All Over the World , IntechOpen , Available at: https://www.intechopen.com/chapters/59075 . Search in Google Scholar

Livak, K. J. and Schmittgen, T. D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408. Livak K. J. and Schmittgen T. D. 2001 . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method . Methods 25 : 402 – 408 . 10.1006/meth.2001.126211846609 Search in Google Scholar

Lozano-Torres, J. L., Wilbers, R. H., Warmerdam, S., Finkers-Tomczak, A., Diaz-Granados, A., van Schaik, C. C., Helder, J., Bakker, J., Goverse, A., Schots, A. and Smant, G. 2014. Apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors. PLoS Pathogens 10:e1004569. Lozano-Torres J. L. Wilbers R. H. Warmerdam S. Finkers-Tomczak A. Diaz-Granados A. van Schaik C. C. Helder J. Bakker J. Goverse A. Schots A. and Smant G. 2014 . Apoplastic venom allergen-like proteins of cyst nematodes modulate the activation of basal plant innate immunity by cell surface receptors . PLoS Pathogens 10 : e1004569 . 10.1371/journal.ppat.1004569426376825500833 Search in Google Scholar

Nguyen, C.- N., Perfus-Barbeoch, L., Quentin, M., Zhao, J., Magliano, M., Marteu, N., Da Rocha, M., Nottet, N., Abad, P. and Favery, B. 2018. A root-knot nematode small glycine and cysteine-rich secreted effector, MiSGCR1, is involved in plant parasitism. New Phytologist 217:687–699. Nguyen C.- N. Perfus-Barbeoch L. Quentin M. Zhao J. Magliano M. Marteu N. Da Rocha M. Nottet N. Abad P. and Favery B. 2018 . A root-knot nematode small glycine and cysteine-rich secreted effector, MiSGCR1, is involved in plant parasitism . New Phytologist 217 : 687 – 699 . 10.1111/nph.1483729034957 Search in Google Scholar

Nielsen, H. 2017. Predicting Secretory Proteins with SignalP. Methods in Molecular Biology 1611:59–73. Nielsen H. 2017 . Predicting Secretory Proteins with SignalP . Methods in Molecular Biology 1611 : 59 – 73 . 10.1007/978-1-4939-7015-5_628451972 Search in Google Scholar

Paganini, J., Campan-Fournier, A., Da Rocha, M., Gouret, P., Pontarotti, P., Wajnberg, E., Abad, P. and Danchin, E. G. J. 2012. Contribution of lateral gene transfers to the genome composition and parasitic ability of root-knot nematodes. PLoS ONE 7:e50875. Paganini J. Campan-Fournier A. Da Rocha M. Gouret P. Pontarotti P. Wajnberg E. Abad P. and Danchin E. G. J. 2012 . Contribution of lateral gene transfers to the genome composition and parasitic ability of root-knot nematodes . PLoS ONE 7 : e50875 . 10.1371/journal.pone.0050875351127223226415 Search in Google Scholar

Petitot, A. S., Dereeper, A., Agbessi, M., Da Silva, C., Guy, J., Ardisson, M. and Fernandez, D. 2016. Dual RNA-seq reveals Meloidogyne graminicola transcriptome and candidate effectors during the interaction with rice plants. Molecular Plant Pathology 17:860–874. Petitot A. S. Dereeper A. Agbessi M. Da Silva C. Guy J. Ardisson M. and Fernandez D. 2016 . Dual RNA-seq reveals Meloidogyne graminicola transcriptome and candidate effectors during the interaction with rice plants . Molecular Plant Pathology 17 : 860 – 874 . 10.1111/mpp.12334663836126610268 Search in Google Scholar

Phan, N. T., Orjuela, J., Danchin, E. G. J., Klopp, C., Perfus-Barbeoch, L., Kozlowski, D. K., Koutsovoulos, G. D., Lopez-Roques, C., Bouchez, O., Zahm, M., Besnard, G. and Bellafiore, S. 2020. Genome structure and content of the rice root-knot nematode (Meloidogyne graminicola). Ecology and Evolution 10:11006–11021. Phan N. T. Orjuela J. Danchin E. G. J. Klopp C. Perfus-Barbeoch L. Kozlowski D. K. Koutsovoulos G. D. Lopez-Roques C. Bouchez O. Zahm M. Besnard G. and Bellafiore S. 2020 . Genome structure and content of the rice root-knot nematode (Meloidogyne graminicola) . Ecology and Evolution 10 : 11006 – 11021 . 10.1002/ece3.6680759317933144944 Search in Google Scholar

Pinkerton, J. N., Santo, G. S., Ponti, R. P. and Wilson, J. S. 1986. Control of Meloidogyne chitwoodi in commercially grown Russet Burbank potatoes. Plant Disease 70:860–863. Pinkerton J. N. Santo G. S. Ponti R. P. and Wilson J. S. 1986 . Control of Meloidogyne chitwoodi in commercially grown Russet Burbank potatoes . Plant Disease 70 : 860 – 863 . 10.1094/PD-70-860 Search in Google Scholar

Porter, I., Banks, J., Mattner, S. and Fraser, P. 2009. “Global phaseout of methyl bromide under the Montreal protocol: implications for bioprotection, biosecurity and the ozone layer”, In Gisi, U., Chet, I. and Gullino, M. L. (Eds), Recent Developments in Management of Plant Diseases, Springer Netherlands, Dordrecht, pp. 293–309. Porter I. Banks J. Mattner S. and Fraser P. 2009 . “Global phaseout of methyl bromide under the Montreal protocol: implications for bioprotection, biosecurity and the ozone layer” , In Gisi U. Chet I and Gullino M. L. (Eds), Recent Developments in Management of Plant Diseases , Springer Netherlands , Dordrecht , pp. 293 – 309 . Search in Google Scholar

Roze, E., Hanse, B., Mitreva, M., Vanholme, B., Bakker, J. and Smant, G. 2008. Mining the secretome of the root-knot nematode Meloidogyne chitwoodi for candidate parasitism genes. Molecular Plant Pathology 9:1–10. Roze E. Hanse B. Mitreva M. Vanholme B. Bakker J. and Smant G. 2008 . Mining the secretome of the root-knot nematode Meloidogyne chitwoodi for candidate parasitism genes . Molecular Plant Pathology 9 : 1 – 10 . Search in Google Scholar

Shukla, N., Yadav, R., Kaur, P., Rasmussen, S., Goel, S., Agarwal, M., Jagannath, A., Gupta, R. and Kumar, A. 2018. Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-infected tomato (Solanum lycopersicum) roots reveals complex gene expression profiles and metabolic networks of both host and nematode during susceptible and resistance responses. Molecular Plant Pathology 19:615–633. Shukla N. Yadav R. Kaur P. Rasmussen S. Goel S. Agarwal M. Jagannath A. Gupta R. and Kumar A. 2018 . Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-infected tomato (Solanum lycopersicum) roots reveals complex gene expression profiles and metabolic networks of both host and nematode during susceptible and resistance responses . Molecular Plant Pathology 19 : 615 – 633 . 10.1111/mpp.12547663813628220591 Search in Google Scholar

Trapnell, C., Roberts, A., Goff, L., Pertea, G., Kim, D., Kelley, D. R., Pimentel, H., Salzberg, S. L., Rinn, J. L. and Pachter, L. 2012. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nature Protocols 7:562–578. Trapnell C. Roberts A. Goff L. Pertea G. Kim D. Kelley D. R. Pimentel H. Salzberg S. L. Rinn J. L. and Pachter L. 2012 . Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks . Nature Protocols 7 : 562 – 578 . 10.1038/nprot.2012.016333432122383036 Search in Google Scholar

Vieira, P. and Gleason, C. 2019. Plant-parasitic nematode effectors-insights into their diversity and new tools for their identification. Current Opinion in Plant Biology 50:37–43. Vieira P. and Gleason C. 2019 . Plant-parasitic nematode effectors-insights into their diversity and new tools for their identification . Current Opinion in Plant Biology 50 : 37 – 43 . 10.1016/j.pbi.2019.02.00730921686 Search in Google Scholar

Wang, X., Xue, B., Dai, J., Qin, X., Liu, L., Chi, Y., Jones, J. T. and Li, H. 2018. A novel Meloidogyne incognita chorismate mutase effector suppresses plant immunity by manipulating the salicylic acid pathway and functions mainly during the early stages of nematode parasitism. Plant Pathology 67:1436–1448. Wang X. Xue B. Dai J. Qin X. Liu L. Chi Y. Jones J. T. and Li H. 2018 . A novel Meloidogyne incognita chorismate mutase effector suppresses plant immunity by manipulating the salicylic acid pathway and functions mainly during the early stages of nematode parasitism . Plant Pathology 67 : 1436 – 1448 . 10.1111/ppa.12841 Search in Google Scholar

Westermann, A. J., Gorski, S. A. and Vogel, J. 2012. Dual RNA-seq of pathogen and host. Nature Reviews Microbiology 10:618–630. Westermann A. J. Gorski S. A. and Vogel J. 2012 . Dual RNA-seq of pathogen and host . Nature Reviews Microbiology 10 : 618 – 630 . 10.1038/nrmicro285222890146 Search in Google Scholar

Zasada, I. A., Dandurand, L. M., Gleason, C., Hagerty, C. H. and Ingham, R. E. 2018. “Plant Parasitic Nematodes of the Pacific Northwest: Idaho”, In S. S. and C. J. (Eds), Oregon and Washington, Plant Parasitic Nematodes in Sustainable Agriculture of North America, Springer, Cham. Zasada I. A. Dandurand L. M. Gleason C. Hagerty C. H. and Ingham R. E. 2018 . “Plant Parasitic Nematodes of the Pacific Northwest: Idaho” , In S. S. and C. J. (Eds), Oregon and Washington , Plant Parasitic Nematodes in Sustainable Agriculture of North America , Springer , Cham . 10.1007/978-3-319-99585-4_8 Search in Google Scholar