Effect of fluensulfone on different functional genes of root-knot nematode

	Nematode mortality (%)
Fluensulfone concentration (ppm)	1hr	2.5hr	5hr	7.5hr	10hr	24hr	Remarks
100	0.86	3.47	8.62	18.51	27.78	100	Reduced motility and agility observed 0.5hr onward the drug treatment
75	0	1.74	4.35	8.62	18.18	100	Reduced motility and agility observed 1hr onward the drug treatment
50	0	1.74	8.08	13.79	13.79	100	Reduced motility and agility observed 1hr onward the drug treatment
25	0	0.86	2.59	8.62	11.01	90.74	Reduced motility and agility observed 4hr onward the drug treatment
10	0	0	0	2.59	6.03	75.92	Reduced motility and agility observed 4hr onward the drug treatment
5	0	0	0	1.74	1.74	43.48	Reduced motility and agility observed 7hr onward the drug treatment
1	0	0	0	0	1.74	29.57	Reduced motility and agility in few worms observed 7–8hr onward the drug treatment
Vehicle control (0.05% DMSO)	0	0	0	0	0	0	No change in motility and agility

List of primers used in this study.

Sl	Primers name	Gene name (accession number)	Primers Sequences (5′–3′)	Tm (°C)	Purpose
1	RT miODR-1 F	Mi-odr-1 (MG780832)	GAACCACGCTCTTACGATTC	60°C	qRT PCR
	RT miODR-1 R		CTCCAGAAGCGACCATGTA
2	RT miODR-3 F	Mi-odr-3 (MG780833)	CTGGCTATAGACCCACAGAA	60°C	qRT PCR
	RT miODR-3 R		GAACGTTGTCCACCTACATC
3	RT miTAX-2 F	Mi-tax-2 (MG780834)	GAGGGAATATCCTGAAGCG	60°C	qRT PCR
	RT miTAX-2 R		CCTGATTCCACTGTTCTGG
4	RT miTAX-4 F	Mi-tax-4 (MG780835)	GTGAAGTTCCTTGGCCTAT	60°C	qRT PCR
	RT miTAX-4 R		CAGAGCTAGCAATCATACTC C
5	RT miOSM-9 F	M. incognita osm-9 (MT676864)	CATGCCTGAAGATTGGGAAG	60°C	Cloning and qRT PCR
	RT miOSM-9 R		GGTCAGGATTAGCACCATAC
6	RT miGPC-1 F	M. incognita gpc-1 (MT676857)	CAACTTCGACAAGAGGCAA	60°C	Cloning and qRT PCR
	RT miGPC-1 R		CCCTATTGGTCCGGTAATTAAG
7	RT mi GPA-11F	M. incognita gpa-11 (MT676854)	CGACTACGGGTATTTGTGAC	60°C	Cloning and qRT PCR
	RT miGPA-11 R		GAAAGTTCGGCTACGAACAG
8	RT miGPA-13 F	M. incognita gpa-13 (MT676855)	GCTGAAACTTCAGAGGATGG	60°C	Cloning and qRT PCR
	RT miGPA-13 R		GCTGGTATTCACGAGAACG
9	RT miGRK-2 F	M. incognita grk-2 (MT676858)	CTCAATTCTACGCAGCAGAG	60°C	Cloning and qRT PCR
	RT miGRK-2 R		GGTGCCATATAGCCAACAG
10	RT miXYL-1 F	Mi-xylanase (AF224342)	GGGATTAGTTGCATACAGTTAAGATAAG	60°C	qRT PCR
	RT miXYL-1 R		GCTGTTCGAGTAAGCAGTAGAG
11	RT miXYL-3 F	Mi-xylanase (EU475876)	GGAACTTCCGGTAAACCCTA	60°C	qRT PCR
	RT miXYL-3 R		GACCGGACTGATGTGTTATC
12	RT miENG-1 F	Mi-β-1,4-endoglucanase (AF100549)	ACCGAGCAACTCACAAAC	60°C	qRT PCR
	RT miENG-1 R		GGCATTGCTACCCGTATTT
13	RT miPEL F	Mi-pectatelyase (AF527788)	CGAATAACGACGAAGAGGAC	60°C	qRT PCR
	RT miPEL R		AGAACCGACAACCCTACA
14	RT miMSP-20 F	Mi-msp-20 (AY134439)	TGGTGACGAACGCACACCTACATA	60°C	qRT PCR
	RT miMSP-20 R		GCGCTGTCTTTGACCATTTGCTCT
15	RT miMSP-33 F	Mi-msp-33 (AY142118)	GTGGCCTCCTTTGCTTGGACATTT	60°C	qRT PCR
	RT miMSP-33 R		CATCACCTCCAATTACTCCGGGTT
16	RT miFAT-6 F	M. incognita fat-6 (MT676846)	GAATTACTGCAGGTCCTCAC	60°C	Cloning and qRT PCR
	RT miFAT-6 R		CATCAGTGTCAGTCCACTTG
17	RT miACS-2 F	M. incognita acs-2 (MT676837)	CCCACCAGAGGAAAGGATAA	60°C	Cloning and qRT PCR
	RT miACS-2 R		CGCTCCTATACCAACGATCT
18	RT miMDT-15 F	M. incognita mdt-15 (MT676859)	CAGCTATGCCACCAGGTTT	60°C	Cloning and qRT PCR
	RT miMDT-15 R		CCTAATCCTCCTCCACCATTTC
19	RT miNHR-49 F	M. incognita nhr-49 (MT676861)	GCCAATGAGGCAATTAGAG	60°C	Cloning and qRT PCR
	RT miNHR-49 R		CCTCGTCTACCGGTTTAGAT
20	RT miELO-2 F	M. incognita elo-2 (MT676842)	CAAACGGATATTGGGTCTGG	60°C	Cloning and qRT PCR
	RT miELO-2 R		GGCGTGATAGGGTAGGAATA
21	RT miECH-5 F	M. incognita ech-5 (MT676839)	CACTAGGTGGAGGACTAGAA	60°C	Cloning and qRT PCR
	RT miECH-5 R		CTTGGGAGTCTTTGAGTTCC
22	RT miECH-6 F	M. incognita ech-6 (MT676840)	CGAGTCTTCTGGAAGGGATA	60°C	Cloning and qRT PCR
	RT miECH-6 R		GCTTAGATGGCAAAGAGGTC
23	RT miFLP-12 F	Mi-flp-12 (AY804187)	TGAGGAAGCGGCCCGATAGTTCTT	60°C	qRT PCR
	RT miFLP-12 R		GGATGAAGAAATGCTTGGACGAGT
24	RT miFLP-14 F	Mi-flp-14 (AY907829)	GCGAGTCCATGTGTAGCAGCTAAT	60°C	qRT PCR
	RT miFLP-14 R		GGGAGATGAAGAACGTTTACTACTTTGCC
25	RT miFLP-16 F	Mi-flp-16 (EU549831)	GGCAATATTCACGAGACTGGCAAC	60°C	qRT PCR
	RT miFLP-16 R		GGCCATTCAATGCCTGAAGAGGG
26	RT miFLP-18 F	Mi-flp-18 (AY729022)	AGGATGACTTATTGCGCCAGGA	60°C	qRT PCR
	RT miFLP-18 R		TTCCTTTACCGAATCTGAGCACGC
27	RT miACE-1 F	Mi-ace-1 (AF075718)	CTCCTTGTTCTGAGGATTGTCT	60°C	qRT PCR
	RT miACE-1 R		TTTATGGAGGTGGATTCTGGAG
28	RT miACE-2 F	Mi-ace-2 (AF495588)	AGATGTGGAATCCGCCTAATG	60°C	qRT PCR
	RT miACE-2 R		TTTACTCGGGCTCTCCTTCT
29	RT miNLP-3 F	Mi-nlp-3 (KY054882)	TCCACAATTTGTGCCAGGTC	60°C	qRT PCR
	RT miNLP-3 R		TTGTGGGGCTGATCGTTTTC
30	RT miNLP-12 F	Mi-nlp-12 (KY054885)	GATAGGCGAAAGCGGACTATT	60°C	qRT PCR
	RT miNLP-12 R		CAAGGGCCTGTAATCTCTCTTT
31	RT mi18S rRNA F	Mi-18S rRNA (HE667742)	TCAACGTGCTTGTCCTACCCTGAA	60°C	qRT PCR
	RT mi18S rRNA R		TGTGTACAAAGGGCAGGGACGTAA
32	RT mi actin F	Mi-actin (BE225475)	TGACTCTGGAGATGGTGTTACG	60°C	qRT PCR
	RT mi actin R		GTGATGACTTGACCGTCAGGC

Homology parameters of unannotated genes used in this study.

Sl	Primers name	Gene name (accession number)	Primers Sequences (5′–3′)	Amplicon length (bp)	Identity (%)	Score	E-value	Purpose
1	RT miOSM-9 F	M. incognita osm-9 homologue	CATGCCTGAAGATTGGGAAG	150	100	124	3.6E‒62	qRT PCR
	RT miOSM-9 R		GGTCAGGATTAGCACCATAC
2	RT miGPC-1 F	M. incognita gpc-1 homologue	CAACTTCGACAAGAGGCAA	117	100	199	1.4E‒18	qRT PCR
	RT miGPC-1 R		CCCTATTGGTCCGGTAATTAAG
3	RT mi GPA-11F	M. incognita gpa-11 homologue	CGACTACGGGTATTTGTGAC	143	100	58	8.2E‒23	qRT PCR
	RT miGPA-11 R		GAAAGTTCGGCTACGAACAG
4	RT miGPA-13 F	M. incognita gpa-13 homologue	GCTGAAACTTCAGAGGATGG	112	100	194	1.1E‒17	qRT PCR
	RT miGPA-13 R		GCTGGTATTCACGAGAACG
5	RT miGRK-2 F	M. incognita grk-2 homologue	CTCAATTCTACGCAGCAGAG	124	100	202	4.4E‒19	qRT PCR
	RT miGRK-2 R		GGTGCCATATAGCCAACAG
6	RT miFAT-6 F	M. incognita fat-6 homologue	GAATTACTGCAGGTCCTCAC	162	100	306	1.9E‒33	qRT PCR
	RT miFAT-6 R		CATCAGTGTCAGTCCACTTG
7	RT miACS-2 F	M. incognita acs-2 homologue	CCCACCAGAGGAAAGGATAA	200	100	356	4.2E‒40	qRT PCR
	RT miACS-2 R		CGCTCCTATACCAACGATCT
8	RT miMDT-15 F	M. incognita mdt-15 homologue	CAGCTATGCCACCAGGTTT	100	100	105	2.5E‒5	qRT PCR
	RT miMDT-15 R		CCTAATCCTCCTCCACCATTTC
9	RT miNHR-49 F	M. incognita nhr-49 homologue	GCCAATGAGGCAATTAGAG	179	100	294	9.4E‒32	qRT PCR
	RT miNHR-49 R		CCTCGTCTACCGGTTTAGAT
10	RT miELO-2 F	M. incognita elo-2 homologue	CAAACGGATATTGGGTCTGG	148	100	64	2.2E‒26	qRTPCR
	RT miELO-2 R		GGCGTGATAGGGTAGGAATA
11	RT miECH-5 F	M. incognita ech-5 homologue	CACTAGGTGGAGGACTAGAA	130	100	209	4.7E‒20	qRT PCR
	RT miECH-5 R		CTTGGGAGTCTTTGAGTTCC
12	RT miECH-6 F	M. incognita ech-6 homologue	CGAGTCTTCTGGAAGGGATA	193	100	342	1.3E‒36	qRT PCR
	RT miECH-6 R		GCTTAGATGGCAAAGAGGTC

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Effect of fluensulfone on different functional genes of root-knot nematode Meloidogyne incognita

Pubblicato online: 17 ago 2021

Pagine: 1 - 14

DOI: https://doi.org/10.21307/jofnem-2021-073

Parole chiaveChemosensation, Fluensulfone, Metabolism, Neurotransmission, Parasitism, Root-knot nematode

© 2021 Alkesh Hada et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Assessment of mortality of M. incognita J2s at variable concentrations of fluensulfone and time of exposure.

List of primers used in this study.

Homology parameters of unannotated genes used in this study.

Parole chiave
Chemosensation, Fluensulfone, Metabolism, Neurotransmission, Parasitism, Root-knot nematode