First record of native entomopathogenic nematodes from Montana agroecosystems

Character	Steinernema feltiae 1	Steinernema feltiae 2	ANOVA results	Heterorhabditis bacteriophora
L	820.25±11.16 (711-900)^a	807.85±9.60 (743-906)^a	t=0.82, df=37, P=0.11	602.25±9.48 (513-654)
D	28.48±0.73 (22-34.4)^a	29.93±0.57 (26-35)^a	t=−1.63, df=36, P=0.11	24.74±0.64 (18-31)
EP	64.45±1.94 (48-80)^a	55.25±1.72 (43-67)^b	t=3.45, df=37, P=0.001	77.35±2.24 (55-95)
NR	72±2.83 (42-91)^a	59.65±2.01 (48-81)^b	t=3.47, df=34, P=0.001	78.2±1.32 (68-90)
ES	90.2±2.97 (61-114)^a	87.7±2.02 (48-81)^a	t=0.68, df=33, P=0.50	106.5±3.10 (93-140)
T	79.34±1.49 (61-90)^a	80.85±1.09 (74-89)^a	t=−0.82, df=35, P=0.42	91.26±4.52 (56-140.3)
A	29.15±0.81 (24.58-39.68)^a	27.19±0.6 (23.23-32.78)^a	t=1.90, df=36, P=0.07	24.34±0.60 (14.93-28.5)
B	9.31±0.36 (7.45-13.15)^a	9.31±0.25 (7.40-11.13)^a	t=0.0, df=34, P=1.00	5.65±0.18 (3.063-5.52)
C	10.40±0.21 (8.67-13.14)^a	10.02±0.16 (8.78-11.19)^a	t=1.63, df=37, P=0.11	6.60±0.29 (2.10-9.16)
D%	72.42±2.36 (55.24-87.91)^a	63.62±2.37 (39.81-83.33)^a	t=−1.63, df=36, P=0.11	72.63±2.61 (72.29-59.14)
E%	81.60±2.63 (63.16-108.11)^a	68.42±2.11 (51.81-86.84)^b	t=3.82, df=36, P=0.0005	84.75±5.04 (49.52-98.21)

Morphological characteristics of males of three entomopathogenic nematode isolates from Montana, USA.

Character	Steinernema feltiae 1 (1st gen.)	Steinernema feltiae 2 (1st gen.)	t-test results	Heterorhabditis bacteriophora (2nd gen.)
L	1570.35±36.84 (1259-1813)^a	1304.8±43.3 (913-1570)^b	t=4.55, df=37, P<0.0001	802.1±20.16 (610-920)
D	96.93±3.01 (76-130)^a	96.05±3.14 (65-119)^a	t=0.20, df=38, P=0.85	62.3±1.99 (50-78)
EP	90.06±2.25 (73-115)^a	93±1.55 (84-109)^a	t=−1.05, df=34, P=0.30	77.9±1.17 (70-90)
NR	98.35±1.63 (88-113)^a	100±1.18 (88-110)^a	t=−0.80, df=35, P=0.43	80.45±1.32 (70-91)
ES	145.55±2.80 (121-167)^b	164.55±1.72 (154-180)^a	t=−5.63, df=32, P<0.0001	110.25±1.97 (97-129)
T	41.65±0.75 (36-48)^b	52.75±2.62 (34-72) ^a	t=−3.96, df=22, P=0.0006	61.58±3.59 (41-110)
ABD	44.72±1.07 (35-51)^b	62.85±3.00 (45-91)^a	t=−5.56, df=24, P<0.0001	34.51±0.67 (30-39.4)
SP	80.35±1.41 (67-92)^a	71.5±2.21 (58-98)^b	t=4.49, df=36, P<0.0001	41±1.26 (32-51)
GU	49.95±1.11 (41-59) ^a	45.85±0.86 (40-53) ^b	t=2.85, df=36, P=0.007	22.95±0.74 (14-28)
D%	62.09±1.47 (49.08-72.34)^a	56.56±0.86 (51.14-65.19)^a	t=0.20, df=38, P=0.85	70.90±1.19 (63.2-83.50)
SW%	181.27±4.51 (145.65-235.90)^a	117.34±6.81 (63.74-168)^a	t=7.63, df=33, P<0.0001	119.83±4.69 (91.37-164.52)
GS%	62.74±2.13 (49.40-85.51)^a	66.35±2.16 (51.19-91.38)^a	t=−1.16, df=38, P=0.25	56.85±2.61 (41.18-87.5)

Different agricultural field sites surveyed in Montana, USA, for native entomopathogenic nematodes during 2018.

Sampling site	Farmer	Latitude	Longitude	Elevation (m)	Vegetation	Soil texture	Soil pH	Organic Matter (%)	Presence/Absence of EPNs	Re-culture^*
Pendroy	John Stoltz	N48.04130°	W112.16945°	4220	Wheat/Canola	Clay loam	8.2	3.9	+(3)	No
Pendroy	Kevin Johnson	N48.04206°	W112.20099°	4328	Wheat	Sandy clay loam	7.9	4.4	+(1)	No
Choteau	Joe Miller	N47.56785°	W112.13926°	3906	Fallow	Clay	7.8	3.2	−	−
Choteau	Mike Leys	N47.90238°	W112.3802°	−	Wheat	Sandy clay loam	8	3.3	+(2)	Yes (1 Steinernematid and 1 Heterorhabditid)
Valier	John Majerus	N48.18454°	W111.55524°	−	Wheat	Sandy clay loam	8.1	3.4	−
Conrad	Mark Grub	N48.6334°	W111.53175°	−	Peas	Sandy clay loam	7	2.9	−	−
Conrad	Mark Orcutt	N48.16259°	W111.52896°	3498	Fallow	Clay loam	8.1	2.3	−	−
Conrad	Garett Grub	N47.56786°	W112.13930°	−	Wheat/Vegetables	Sandy clay loam	7.8	2.4	+(2)	No
Kalispell	Ron De Yong	N48.1945°	W114.2341°	−	Fallow/Winter wheat	Loam	8.1	3.4	+(1)	Yes (1 Steinernematid)
Valier	Jeremy Curry	N48.3032°	W112.4055°	3749	Fallow/Wheat	Sandy clay loam	8.2	2.1	+(2)	No
Conrad	Zane Drishinski	N48.77764°	W111.8948°	3508	Alfalfa	Clay loam	6.8	2.8	−	−
Conrad	WTARC	N48.3076°	W111.9255°	−	Fallow/Wheat	Clay loam	7.8	2.9	−	−
Conrad	Garman	N48.27808°	W111.92616°	1116.05	Alfalfa	Clay loam	8.1	3.7	−	−
Choteau (Knees)	A Killion	N48.00729°	W111.36596°	1081.26	Chickpea	Clay loam	8.2	2.5	−	−
Knees	A Killion	N48.00729°	W111.36596°	1081.26	Flax	Clay loam	7.5	3.5	+(1)	No
Brady	Winterhood	N47.98468°	W111.84313°	1112.80	Fallow/Winter wheat	Sandy clay loam	7.3	1.9	+(1)	No
Collins	Chickenhead	N47.95588°	W111.90971°	1122.68	Chickpea	Clay	7.8	2.3	+(1)	No
Choteau		N47.91965°	W112.04917°	1177.36	Hemp	Clay	8.2	2.8	−	−
Choteau	Nesbitt	N47.79007°	W111.16589°	1224.32	Durum Wheat	Clay loam	6.6	4.4	−	−
Dutton	Tims	N47.83529°	W111.53990°	1144.20	Lentils	Clay loam	7.9	3.4	−	−
Shelby west	John Wigand	N48.56643°	W111.98537°	1103.57	Peas	Sandy clay loam	6.9	3.9	−	−
Shelby west	John Wigand	N48.57870°	W111.99317°	1087.12	Triticale	Sandy loam	7.6	2.6	−	−
Shelby West	John Wigand	N48.54374°	W111.99096°	1071.82	Fallow	Clay loam	7.6	2.3	−	−
Potter Rd Shelby	John Wigand	N48.65339°	W111.96044°	1057.40	Fallow	Clay loam	7.9	2.7	−	−
Sunburst	Koeye Fauque	N48.82692°	W111.85641°	1093.77	Chickpea	Sandy clay loam	8	2.9	+(1)	No
Sunburst	Koeye Fauque	N48.82701°	W111.81002°	1102.46	Spring wheat	Clay loam	8.2	2.3	−	−
Tiber	Duncan	N48.19011°	W111.25206°	993.03	Fallow	Clay loam	8.2	2.6	−	−
Tiber	Paul Kolstad	N48.17184°	W111.17372°	960.91	Fallow	Clay loam	8.1	2.3	−	−
Tiber	Wicks	N48.23188°	W111.14511°	1029.37	Lentil/Fallow	Clay loam	7.7	2.5	−	−
Tiber	Broadhurst	N48.27280°	W111.14571°	930.70	Fallow	Clay loam	8.1	2.6	−	−

eISSN:: 2640-396X
Lingua:: Inglese

Frequenza di pubblicazione:: Volume Open
Argomenti della rivista:: Life Sciences, other

Feed RSS della rivista

First record of native entomopathogenic nematodes from Montana agroecosystems

Pubblicato online: 06 lug 2020

Pagine: 1 - 11

Ricevuto: 27 dic 2019

DOI: https://doi.org/10.21307/jofnem-2020-060

Parole chiaveEntomopathogenic nematodes, Golden Triangle Region of Montana, Heterorhabditis, Steinernema

© 2020 Ramandeep K. Sandhi et al., published by Sciendo.

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Morphological characters of 3rd stage infective juveniles for three entomopathogenic nematode isolates from Montana, USA.

Morphological characteristics of males of three entomopathogenic nematode isolates from Montana, USA.

Different agricultural field sites surveyed in Montana, USA, for native entomopathogenic nematodes during 2018.

Parole chiave
Entomopathogenic nematodes, Golden Triangle Region of Montana, Heterorhabditis, Steinernema