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Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet

Richard A. Quick

Richard A. Quick

Temperate Tree Fruit and Vegetable Research Unit, USDA-Agricultural Research ServiceProsser, US
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Quick, Richard A.
, 
Launa Cimrhakl

Launa Cimrhakl

Irrigation Agriculture Research and Extension Center, Washington State UniversityProsser, US
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Cimrhakl, Launa
, 
Hassan Mojtahedi

Hassan Mojtahedi

AgNema LLCRichland, US
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Mojtahedi, Hassan
, 
Vidyasagar Sathuvalli

Vidyasagar Sathuvalli

Hermiston Agricultural Research and Extension Center, Oregon State UniversityHermiston, US
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Sathuvalli, Vidyasagar
, 
Maximilian J. Feldman

Maximilian J. Feldman

  • Temperate Tree Fruit and Vegetable Research Unit, USDA-Agricultural Research ServiceProsser, US
  • Irrigation Agriculture Research and Extension Center, Washington State UniversityProsser, US
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Feldman, Maximilian J.
 e 
Charles R. Brown

Charles R. Brown

  • Temperate Tree Fruit and Vegetable Research Unit, USDA-Agricultural Research ServiceProsser, US
  • Irrigation Agriculture Research and Extension Center, Washington State UniversityProsser, US
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Brown, Charles R.
  
20 mar 2020
Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet's Cover Image
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Pubblicato online: 20 mar 2020
Pagine: 1 - 10
Ricevuto: 12 ago 2019
DOI: https://doi.org/10.21307/jofnem-2020-011
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© 2020 Richard A. Quick et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Two weeks post-transplant, plant hosts were inoculated to evaluate their ability to host Stubby root nematodes (SRN) and Tobacco rattle virus (TRV). At month zero, test hosts were transplanted into pots containing viruliferous SRNs and cultured for one to three months. Each month a subset of hosts is sacrificed, nematode counts are taken, and a tobacco bait plant is transplanted into the test pot. After one month the tobacco bait plant is harvested and both foliar and root tissue was tested for the presence of TRV using RT-PCR.
Two weeks post-transplant, plant hosts were inoculated to evaluate their ability to host Stubby root nematodes (SRN) and Tobacco rattle virus (TRV). At month zero, test hosts were transplanted into pots containing viruliferous SRNs and cultured for one to three months. Each month a subset of hosts is sacrificed, nematode counts are taken, and a tobacco bait plant is transplanted into the test pot. After one month the tobacco bait plant is harvested and both foliar and root tissue was tested for the presence of TRV using RT-PCR.

Figure 2:

Population counts of stubby root nematode, Paratrichodorus allius, across three months in pots grown with alfalfa, Russet Burbank potato, Castle Russet potato and tobacco; inoculated with 60 and 1,060 nematodes/10 L soil in Experiment 1 and 2, respectively.
Population counts of stubby root nematode, Paratrichodorus allius, across three months in pots grown with alfalfa, Russet Burbank potato, Castle Russet potato and tobacco; inoculated with 60 and 1,060 nematodes/10 L soil in Experiment 1 and 2, respectively.

Figure 3:

Number of bait plant tissue samples that tested positive and negative for the presence of TRV by RT-PCR. (A) Foliage tissue in Experiment 1 (B) Root tissue in Experiment 1 (C) Foliage tissue in Experiment 2 (D) Root tissue in Experiment 2.
Number of bait plant tissue samples that tested positive and negative for the presence of TRV by RT-PCR. (A) Foliage tissue in Experiment 1 (B) Root tissue in Experiment 1 (C) Foliage tissue in Experiment 2 (D) Root tissue in Experiment 2.

Figure A1:

Nematode reproduction factor (Rf) estimates calculated from 250 cm3 soil samples collected from four plant genotypes, across three months, in two independent experiments. Individual data points are plotted as circular dots, whereas the population mean is plotted using an “X” symbol and standard error of the mean is denoted by the error bars. Experiments are differentiated by inoculation pressure. (A) Experiment 1 (60 nematodes/10 L) is plotted in orange (B) Experiment 2 (1,060 nematodes/10 L) plotted in red.
Nematode reproduction factor (Rf) estimates calculated from 250 cm3 soil samples collected from four plant genotypes, across three months, in two independent experiments. Individual data points are plotted as circular dots, whereas the population mean is plotted using an “X” symbol and standard error of the mean is denoted by the error bars. Experiments are differentiated by inoculation pressure. (A) Experiment 1 (60 nematodes/10 L) is plotted in orange (B) Experiment 2 (1,060 nematodes/10 L) plotted in red.

Figure A1:

(continued)
(continued)

Average nematode count, reproduction factor (Rf) values and proportion of foliage and root tissue samples with TRV infection at the final sampling time point_

Plant Experiment Nematode count Rf value Foliage TRV infection (%) Root TRV infection (%)
Alfalfa 1 126.40b 84.26b 0a,b 0b
Burbank 1 20.00d 13.33b 33a,b 50a,b
Castle 1 119.87c 79.91b 0b 0b
Tobacco 1 720.80a 480.53a 80a 100a
Alfalfa 2 6.00d 0.22c 0b 20a
Burbank 2 64b 2.41b 100a 100a
Castle 2 19.6c 0.73b,c 0b 0a
Tobacco 2 768a 28.98a 100a 80a
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