Accesso libero

A new rare nematode Nothocriconemoides hangzhouensis n. sp. (Nematoda: Criconematidae) from Hangzhou, China

Munawar Maria
Munawar Maria
, 
Wentao Miao
Wentao Miao
, 
Ruihang Cai
Ruihang Cai
, 
Pablo Castillo
Pablo Castillo
 e 
Jingwu Zheng
Jingwu Zheng
   | 18 mar 2020
Journal of Nematology's Cover Image
INFORMAZIONI SU QUESTO ARTICOLO
Articolo precedente
Articolo Successivo
Cita
Pubblicato online: 18 mar 2020
Pagine: 1 - 14
Ricevuto: 06 gen 2020
DOI: https://doi.org/10.21307/jofnem-2020-006
Parole chiave
© 2020 Munawar Maria et al., published by Sciendo.
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1:

Line drawings of Nothocriconemoides hangzhouensis n. sp. Female A: esophageal region; B: Cepahlic region; C: En face view: D-F: Cuticle markings; G: Tail region under SEM; H: Tail region under LM; I: Cepahlic region of juvenile: J: Crenation on cuticle of juvenile; K: Tail region of juvenile. (Scale bars = A =50 μm, B-I = 10 μm).
Line drawings of Nothocriconemoides hangzhouensis n. sp. Female A: esophageal region; B: Cepahlic region; C: En face view: D-F: Cuticle markings; G: Tail region under SEM; H: Tail region under LM; I: Cepahlic region of juvenile: J: Crenation on cuticle of juvenile; K: Tail region of juvenile. (Scale bars = A =50 μm, B-I = 10 μm).

Figure 2:

Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Female A: Entire body; B-E: Cepahlic regions; F-H: Esophageal regions, arrow pointing on the excretory pore (exp): I-K: Cuticle markings; L-O: Tail regions, arrows pointing on vulva (v) and anus (a). (Scale bars=A=50 μm, B-I=10 μm).
Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Female A: Entire body; B-E: Cepahlic regions; F-H: Esophageal regions, arrow pointing on the excretory pore (exp): I-K: Cuticle markings; L-O: Tail regions, arrows pointing on vulva (v) and anus (a). (Scale bars=A=50 μm, B-I=10 μm).

Figure 3:

Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Juvenile A: Entire body; B: Cepahlic region; C,D: Esophageal regions: E: Crenation on cuticle; F, G: Tail regions, arrows pointing on anus (a). (Scale bars=A=50 μm, B-I=10 μm).
Light photomicrographs of Nothocriconemoides hangzhouensis n. sp. Juvenile A: Entire body; B: Cepahlic region; C,D: Esophageal regions: E: Crenation on cuticle; F, G: Tail regions, arrows pointing on anus (a). (Scale bars=A=50 μm, B-I=10 μm).

Figure 4:

Scanning electron microscopy of Nothocriconemoides hangzhouensis n. sp. Female. A: Entire body; B-D: En face view; E: Cuticle markings; F-H: tail regions arrows pointing on vulva (v) and anus (a) (Scale bars, A = 100 μm; B, C = 10 μm; D, H = 20 μm; G-F = 30 μm).
Scanning electron microscopy of Nothocriconemoides hangzhouensis n. sp. Female. A: Entire body; B-D: En face view; E: Cuticle markings; F-H: tail regions arrows pointing on vulva (v) and anus (a) (Scale bars, A = 100 μm; B, C = 10 μm; D, H = 20 μm; G-F = 30 μm).

Figure 5:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=7,315.8130; AIC = 14,859.6260; freqA=0.2371; freqC=0.2413; freqG=0.2833; freqT=0.2384; R(a)=1.5166; R(b)=2.2509; R(c)=09364; R(d)=0.7246; R(e)=6.0997; R(f)=1.0000; Pinva=0.6630; and Shape=0.6070). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the 18 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=7,315.8130; AIC = 14,859.6260; freqA=0.2371; freqC=0.2413; freqG=0.2833; freqT=0.2384; R(a)=1.5166; R(b)=2.2509; R(c)=09364; R(d)=0.7246; R(e)=6.0997; R(f)=1.0000; Pinva=0.6630; and Shape=0.6070). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 6:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=8,382.1334; AIC=16,972.2669; freqA=0.1451; freqC=0.2354; freqG=0.3515; freqT=0.2681; R(a)=0.8404; R(b)=2.5613; R(c)=1.6924; R(d)=0.4616; R(e)=4.7092; R(f)=1.0000; Pinva=0.2730; and Shape=0.8370). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the D2-D3 of 28 S rRNA gene sequence dataset with the GTR + I + G model (−lnL=8,382.1334; AIC=16,972.2669; freqA=0.1451; freqC=0.2354; freqG=0.3515; freqT=0.2681; R(a)=0.8404; R(b)=2.5613; R(c)=1.6924; R(d)=0.4616; R(e)=4.7092; R(f)=1.0000; Pinva=0.2730; and Shape=0.8370). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 7:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR + I + G model (−lnL=7727.9982; AIC = 15603.9963; freqA=0.2067; freqC=0.2560; freqG=0.2814; freqT=0.2559; R(a)=1.5781; R(b)=2.9918; R(c)=1.7856; R(d)=0.6423; R(e)=2.8799; R(f)=1.0000; Pinva=0.0460; and Shape=0.6180). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the ITS rRNA gene sequence dataset with the GTR + I + G model (−lnL=7727.9982; AIC = 15603.9963; freqA=0.2067; freqC=0.2560; freqG=0.2814; freqT=0.2559; R(a)=1.5781; R(b)=2.9918; R(c)=1.7856; R(d)=0.6423; R(e)=2.8799; R(f)=1.0000; Pinva=0.0460; and Shape=0.6180). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Figure 8:

Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the coxI gene sequence dataset with the GTR + I + G model (−lnL=13,473.0592; AIC=27,198.1184; freqA=0.3715; freqC=0.0509; freqG=0.0477; freqT=0.5299; R(a)=0.7544; R(b)=36.5547; R(c)=1.6680; R(d)=51.5187; R(e)=20.3538; R(f)=1.0000; Pinva=0.2510; and Shape=0.3470). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.
Phylogenetic relationships of Nothocriconemoides hangzhouensis n. sp. with other criconematids species as inferred from Bayesian analysis using the coxI gene sequence dataset with the GTR + I + G model (−lnL=13,473.0592; AIC=27,198.1184; freqA=0.3715; freqC=0.0509; freqG=0.0477; freqT=0.5299; R(a)=0.7544; R(b)=36.5547; R(c)=1.6680; R(d)=51.5187; R(e)=20.3538; R(f)=1.0000; Pinva=0.2510; and Shape=0.3470). Posterior probability more than 70% is given for appropriate clades. Newly obtained sequences are indicated in bold.

Morphometric data for Nothocriconemoides hangzhouensis n. sp.

Holotype Paratype
n 17
Body Length 494.0 487.1±43.8 (419.6-572.3)
R 36.0 37.2±1.2 (35.0-39.0)
Rst 7.0 6.5±0.5 (6.0-7.0)
Rex 15.0 14.7±0.6 (13.0-15.0)
RV 3.0 2.9±0.2 (2.0-3.0)
Rvan 0.0 0.0±0.0 (0.0-0.0)
Ran 3.0 2.9±0.2 (2.0-3.0)
a 8.2 7.9±0.7 (6.3-9.4)
b 4.2 4.1±0.3 (3.5-4.5)
c 18.6 17.3±1.7 (14.2-20.0)
c' 0.8 0.9±0.1 (0.7-1.1)
V 93.2 92.6±0.9 (90.5-94.0)
VL/VB 0.9 1.0±0.1 (0.9-1.2)
Lip height 8.8 9.5±0.7 (7.8-10.6)
Lip diam. 18.4 20.2±1.4 (17.2-22.1)
Stylet length 71.0 71.1±3.0 (64.4-75.5)
Stylet percentage 14.4 14.7±1.2 (13.1-17.4)
Pharynx length 117.5 118.9±5.0 (111.8-129.6)
Body width 60.3 61.8±5.3 (52.0-69. 4)
Vulval body diam. 37.0 35.7±2.5 (31.8-38.5)
Anal body diam. 31.9 32.1±3.1 (26.4-37.4)
Vulva to tail terminus 33.4 36.0±4.0 (29.8-41.7)
Tail length 26.5 28.3±1.9 (23.3-30.5)
Annuli width 13.1 14.5±1.2 (13.1-16.9)
eISSN:
2640-396X
Lingua:
Inglese
Frequenza di pubblicazione:
Volume Open
Argomenti della rivista:
Life Sciences, other
Feed RSS della rivista