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Morphological and molecular characterization of Labronema montanum sp. n. (Dorylaimida, Dorylaimidae) from Spain

R. Peña-Santiago
R. Peña-Santiago
 e 
J. Abolafia
J. Abolafia
   | 03 giu 2019
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Article Category: Arts & Humanities
Pubblicato online: 03 giu 2019
Pagine: 1 - 11
Ricevuto: 26 mar 2019
DOI: https://doi.org/10.21307/jofnem-2019-029
© 2019 R. Peña-Santiago et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1:

Labronema montanum sp. n. from Spain. (A, B): Anterior region in lateral, median view. (C): Pharyngeal expansion. (D): Female, entire. (E): Male, entire. (F): Female, anterior genital branch. (G): Lip region in lateral, surface view. (H): Pharyngo-intestinal junction. (I): Vagina. (J): Spicule. (K, L): Female tail. (M): Male tail. (N): Lateral guiding piece. (Scale bars: A, B, G, I–M = 10 µm; C, F = 50 µm; D, E = 500 µm; H = 20 µm; N = 5 µm).
Labronema montanum sp. n. from Spain. (A, B): Anterior region in lateral, median view. (C): Pharyngeal expansion. (D): Female, entire. (E): Male, entire. (F): Female, anterior genital branch. (G): Lip region in lateral, surface view. (H): Pharyngo-intestinal junction. (I): Vagina. (J): Spicule. (K, L): Female tail. (M): Male tail. (N): Lateral guiding piece. (Scale bars: A, B, G, I–M = 10 µm; C, F = 50 µm; D, E = 500 µm; H = 20 µm; N = 5 µm).

Figure 2:

Labronema montanum sp. n. from Spain (female, LM). (A): Entire. (B, C): Anterior region in lateral median view. (D): Lip region in lateral, surface view. (E, I): Vagina. (F): Anterior genital branch. (G): Pharyngeal expansion. (H): Pharyngeal enlargement. (J–M): Tail. (Scale bars: A = 500 µm; B–D, H–M = 10 µm; F, G = 50 µm.)
Labronema montanum sp. n. from Spain (female, LM). (A): Entire. (B, C): Anterior region in lateral median view. (D): Lip region in lateral, surface view. (E, I): Vagina. (F): Anterior genital branch. (G): Pharyngeal expansion. (H): Pharyngeal enlargement. (J–M): Tail. (Scale bars: A = 500 µm; B–D, H–M = 10 µm; F, G = 50 µm.)

Figure 3:

Labronema montanum sp. n. from Spain (male, LM). (A): Entire. (B–D): Pharyngo-intestinal junction showing the three lobes. (E): Posterior body region. (F): Spicule. (G, H): Male. (I): Lateral guiding piece. (J): Sperm cells. (Scale bars: A = 500 µm; D–B = 20 µm; E = 50 µm; F–H = 10 µm; I = 5 µm; J = 10 µm).
Labronema montanum sp. n. from Spain (male, LM). (A): Entire. (B–D): Pharyngo-intestinal junction showing the three lobes. (E): Posterior body region. (F): Spicule. (G, H): Male. (I): Lateral guiding piece. (J): Sperm cells. (Scale bars: A = 500 µm; D–B = 20 µm; E = 50 µm; F–H = 10 µm; I = 5 µm; J = 10 µm).

Figure 4:

Labronema montanum sp. n. from Spain (SEM). (A): Lip region in ventral view. (B): Same in frontal view. (C): Vulva, ventral view. (D, E): Male, posterior body region showing ad-cloacal pair (D) and ventromedian series (E) of genital papillae. (F): Female tail, ventral view. (G, H): Female tail, lateral view, showing indentations of body cuticle.
Labronema montanum sp. n. from Spain (SEM). (A): Lip region in ventral view. (B): Same in frontal view. (C): Vulva, ventral view. (D, E): Male, posterior body region showing ad-cloacal pair (D) and ventromedian series (E) of genital papillae. (F): Female tail, ventral view. (G, H): Female tail, lateral view, showing indentations of body cuticle.

Figure 5:

The Bayesian tree inferred from known and the newly sequenced Labronema montanum sp. n. based on sequences of the 28S rRNA region.
The Bayesian tree inferred from known and the newly sequenced Labronema montanum sp. n. based on sequences of the 28S rRNA region.

Morphometrics of Labronema montanum sp. n. from Spain. Measurements in μm except L in mm, and in the form: average ± sd (range).

Holotype Paratypes Paratypes
Character ♀ 17♀♀ 11♂♂
L 1.74 1.83 ± 0.14 (1.56–2.07) 1.87 ± 0.12 (1.68–2.08)
a 17.8 21.3 ± 2.0 (17.8–24.4) 22.7 ± 1.8 (20.3–25.9)
b 3.7 3.8 ± 0.3 (3.3–4.3) 3.7 ± 0.2 (3.3–4.1)
c 62 68.1 ± 9.4 (56–86) 72.2 ± 5.5 (65–84)
V 58 58.4 ± 1.2 (56.5–60.2) –
c’ 0.6 0.6 ± 0.1 (0.5–0.8) 0.6 ± 0.0 (0.6–0.7)
Lip region diameter 23 22.0 ± 1.1 (19–23) 22.2 ± 1.3 (19–24)
Odontostyle length ventral side 27 25.0 ± 1.9 (21–28) 26.0 ± 2.0 (22–29)
Odontostyle length dorsal side 28 26.5 ± 2.1 (23–30) 27.3 ± 2.0 (23–30)
Odontophore length 43 42.0 ± 1.9 (37–44) 42.7 ± 2.2(38–45)
Neck length 474 476 ± 27 (417–514) 500 ± 31 (450–551)
Pharyngeal expansion length 238 242 ± 19 (205–272) 247 ± 16 (212–264)
Body diam. at neck base 92 80.6 ± 8.3 (65–98) 78.9 ± 6.1 (72–91)
 mid-body 98 85.9 ± 7.6 (72–99) 82.8 ± 8.9 (71–101)
 anus/cloaca 44 43.9 ± 3.3 (39–51) 42.3 ± 3.7 (36–47)
Distance vulva – anterior end 1015 1068 ± 88 (889–1232) –
Prerectum length 93 125 ± 23 (84–160) 202 ± 34 (150–268)
Rectum/cloaca length 61 60.0 ± 4.1 (52–68) 71.6 ± 5.6 (61–80)
Tail length 28 27.4 ± 4.5 (19–35) 26.1 ± 2.9 (20–30)
Spicules length – – 71.3 ± 3.6 (65–76)
Ventromedian supplements – – (20–25)
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