<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, <italic>Heterodera carotae</italic> was found parasitizing carrots, <italic>Daucus carota</italic>. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial <italic>COI</italic> genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of <italic>H. carotae</italic> from <italic>H. cruciferae</italic>. The host range test allowed to distinguish these species from each other. Our study showed that male stylet length is longer for <italic>H. carotae</italic> compared with that for <italic>H. cruciferae</italic>. This is an example of importance of combination of several methods for the correct identification of cyst nematodes. To our knowledge, this is the first report of <italic>H. carotae</italic> in Mexico.</p>
</abstract>

During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing carrots, Daucus carota. The nematode was present in 61% of the sampled fields with high population densities, causing severe carrot yield losses in the Tepeaca Valley. The aim of this work was to study morphology, morphometrics, host range, and molecular characterization of the nematode. The morphological and morphometric characterization was made using light and scanning electron microscopy of the second stage juveniles, females, males and cysts, and the host range study, was performed with nine different plants from five families. The molecular identification was made by sequencing and analysing the ITS rRNA and partial COI genes. It was shown that using presently available molecular tools it is not possible to make an accurate differentiation of H. carotae from H. cruciferae. The host range test allowed to distinguish these species from each other. Our study showed that male stylet length is longer for H. carotae compared with that for H. cruciferae. This is an example of importance of combination of several methods for the correct identification of cyst nematodes. To our knowledge, this is the first report of H. carotae in Mexico.

First Report of Carrot Cyst Nematode <italic>Heterodera carotae</italic> in Mexico: Morphological, Molecular Characterization, and Host Range Study

Journal of Nematology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

First Report of Carrot Cyst Nematode  in Mexico: Morphological, Molecular Characterization, and Host Range Study

right-running

During 2008 to 2016 in several nematological surveys in the Tepeaca Valley, Puebla, Mexico, carrot cyst nematode, Heterodera carotae was found parasitizing...

First Report of Carrot Cyst Nematode Heterodera carotae in Mexico: Morphological, Molecular Characterization, and Host Range Study

Article Category: research-article

Pubblicato online: 03 giu 2018

Pagine: 229 - 242

DOI: https://doi.org/10.21307/jofnem-2018-021

Parole chiave
Carrot, COI gene, Morphometrics, Phylogeny, ITS

© 2018 Ilia Mariana Escobar-Avila et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

First Report of Carrot Cyst Nematode Heterodera carotae in Mexico: Morphological, Molecular Characterization, and Host Range Study

Article Category: research-article

Pubblicato online: 03 giu 2018

Pagine: 229 - 242

DOI: https://doi.org/10.21307/jofnem-2018-021

Parole chiaveCarrot, COI gene, Morphometrics, Phylogeny, ITS

© 2018 Ilia Mariana Escobar-Avila et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Parole chiave
Carrot, COI gene, Morphometrics, Phylogeny, ITS