<abstract xmlns="http://www.w3.org/1999/xhtml">Since monoclonal antibodies (Mabs) are potentially available in an unlimited volume, they can be used to screen numerous donor blood samples to identify antigen-negative donors. We have used a Mab (MIMA-9) with characteristics that allow for the simultaneous screening of RBCs of any ABO group for high-incidence antigennegativity in the Kell and Gerbich blood group systems. MIMA-9, a murine IgG2a antibody, previously shown to facilitate the identification of K+k–, Kp(a+b–), K0, McLeod, or Ge:–3 red blood cells (RBCs), was used in MTS gel cards containing anti-mouse IgG as the second antibody to test 1134 K– donors. Among the 1134 donors tested, we found one Kp(a+b–) and one Ge:–2,–3,4 donor. If random donor samples had been used instead of preselecting for K–, we would have expected to identify two K+k– donors. One reagent (MIMA-9) can be used to simultaneously screen for K+k–, Kp(a+b–), K0, McLeod, and Ge:–3 RBCs and thereby conserve rare antisera. Inclusion of anti-mouse IgG in gel cards allowed for rapid screening. MIMA-9 is also a useful reagent to type RBCs with a positive direct antiglobulin test. This antibody is available to donor screening laboratories at no cost for this specific use. <bold><italic>Immunohematology</italic> 2002:18:43–45</bold>.</abstract>

Since monoclonal antibodies (Mabs) are potentially available in an unlimited volume, they can be used to screen numerous donor blood samples to identify antigen-negative donors. We have used a Mab (MIMA-9) with characteristics that allow for the simultaneous screening of RBCs of any ABO group for high-incidence antigennegativity in the Kell and Gerbich blood group systems. MIMA-9, a murine IgG2a antibody, previously shown to facilitate the identification of K+k–, Kp(a+b–), K0, McLeod, or Ge:–3 red blood cells (RBCs), was used in MTS gel cards containing anti-mouse IgG as the second antibody to test 1134 K– donors. Among the 1134 donors tested, we found one Kp(a+b–) and one Ge:–2,–3,4 donor. If random donor samples had been used instead of preselecting for K–, we would have expected to identify two K+k– donors. One reagent (MIMA-9) can be used to simultaneously screen for K+k–, Kp(a+b–), K0, McLeod, and Ge:–3 RBCs and thereby conserve rare antisera. Inclusion of anti-mouse IgG in gel cards allowed for rapid screening. MIMA-9 is also a useful reagent to type RBCs with a positive direct antiglobulin test. This antibody is available to donor screening laboratories at no cost for this specific use. Immunohematology 2002:18:43–45.

MIMA-9, a valuable antibody for screening for rare donors

Immunohematology

{"article-title":"MIMA-9, a valuable antibody for screening for rare donors"}

Since monoclonal antibodies (Mabs) are potentially available in an unlimited volume, they can be used to screen numerous donor blood samples to identify...

MIMA-9, a valuable antibody for screening for rare donors

Pubblicato online: 14 ott 2020

Pagine: 43 - 45

DOI: https://doi.org/10.21307/immunohematology-2019-508

Parole chiave
monoclonal antibody, anti-k, anti-Kp, rare donors

© 2002 E. Tossas et al., published by Sciendo

MIMA-9, a valuable antibody for screening for rare donors

Pubblicato online: 14 ott 2020

Pagine: 43 - 45

DOI: https://doi.org/10.21307/immunohematology-2019-508

Parole chiavemonoclonal antibody, anti-k, anti-Kp, rare donors

© 2002 E. Tossas et al., published by Sciendo

Parole chiave
monoclonal antibody, anti-k, anti-Kp, rare donors