<abstract xmlns="http://www.w3.org/1999/xhtml">Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic transfusion reactions. The molecular basis of the <italic>DOA/DOB</italic> polymorphism is associated with three nucleotide changes: 378C>T, 624 T>C,and 793 A>G of <italic>DO</italic>. While the 378 C>T and 624 T>C are silent mutations, the 793A>G polymorphism in codon 265 encodes asparagine for Doa and aspartic acid for Dob. We describe here the use of a PCR-RFLP assay as an alternative to traditional hemagglutination for typing donor blood for Dombrock. Primers were designed to amplify the region of <italic>DO</italic> containing the 793A>G polymorphism. DNA samples from blood donors were amplified and subjected to RFLP analysis. A total of 613 samples were tested for the Dombrock polymorphism (793 A>G) by PCR-RFLP. PCR-RFLP can be used to screen for Do(a–) or Do(b–) donors. This approach overcomes the scarcity of the reagents required for testing by hemagglutination.</abstract>

Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic transfusion reactions. The molecular basis of the DOA/DOB polymorphism is associated with three nucleotide changes: 378C>T, 624 T>C,and 793 A>G of DO. While the 378 C>T and 624 T>C are silent mutations, the 793A>G polymorphism in codon 265 encodes asparagine for Doa and aspartic acid for Dob. We describe here the use of a PCR-RFLP assay as an alternative to traditional hemagglutination for typing donor blood for Dombrock. Primers were designed to amplify the region of DO containing the 793A>G polymorphism. DNA samples from blood donors were amplified and subjected to RFLP analysis. A total of 613 samples were tested for the Dombrock polymorphism (793 A>G) by PCR-RFLP. PCR-RFLP can be used to screen for Do(a–) or Do(b–) donors. This approach overcomes the scarcity of the reagents required for testing by hemagglutination.

DNA analysis for donor screening of Dombrock blood group antigens

Immunohematology

{"article-title":"DNA analysis for donor screening of Dombrock blood group antigens"}

Due to the scarcity of reliable antibodies, RBC typing for Doa and Dob is notoriously difficult. Inaccurate typing can place patients at risk for hemolytic...

DNA analysis for donor screening of Dombrock blood group antigens

Pubblicato online: 14 ott 2020

Pagine: 73 - 76

DOI: https://doi.org/10.21307/immunohematology-2019-480

Parole chiave
Dombrock blood group system, Dombrock polymorphisms Do/Do, PCR-RFLP, typing donors

© 2003 J.R. Storry et al., published by Sciendo

DNA analysis for donor screening of Dombrock blood group antigens

Pubblicato online: 14 ott 2020

Pagine: 73 - 76

DOI: https://doi.org/10.21307/immunohematology-2019-480

Parole chiaveDombrock blood group system, Dombrock polymorphisms Do/Do, PCR-RFLP, typing donors

© 2003 J.R. Storry et al., published by Sciendo

Parole chiave
Dombrock blood group system, Dombrock polymorphisms Do/Do, PCR-RFLP, typing donors