<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The incidence of weak D has been reported to be between 0.23 and 0.5 percent in Europe and 3.0 percent in the United States. All studies were performed before the introduction of monoclonal anti-D reagents. Using current commercial reagents, this study evaluated D+ samples for the presence of weak D. D+ donors, typed by the Olympus PK 7200, using diluted monoclonal blend anti-D and diluted polyclonal anti-D, were selected by sampling batches of 100 to 200 samples from the previous day’s collection. Anti-D reagents used on the Olympus PK 7200 are required to detect RBCs with the weak D phenotype which do not agglutinate at immediate spin (IS) when tested with polyclonal anti-D by manual tube methods. More than 95 percent of donors tested were Caucasian. Using tube tests with two different monoclonal blend anti-D reagents and one polyclonal anti-D typing reagent, the presence or absence of the D antigen was evaluated after the IS reading. Donors found negative or weakly positive (&lt; 2+) at IS were further typed for weak D by the IAT. The weak D samples were <italic>RHD</italic> genotyped by allele-specific PCR. Of 1005 donors tested, 4 (0.4%) were classified as weak D by one or more anti-D reagents. Polyclonal anti-D reagent demonstrated weaker reactions when compared with the monoclonal blends. All weak D samples were found positive for exon 4, intron 4, and exon 10, a finding consistent with most D+ samples. The incidence of weak D found in this study is not significantly different from that found in earlier studies using polyclonal anti-D reagents. <italic><bold>Immunohematology</bold></italic><bold>2005;21:152–4.</bold></p></abstract>

The incidence of weak D has been reported to be between 0.23 and 0.5 percent in Europe and 3.0 percent in the United States. All studies were performed before the introduction of monoclonal anti-D reagents. Using current commercial reagents, this study evaluated D+ samples for the presence of weak D. D+ donors, typed by the Olympus PK 7200, using diluted monoclonal blend anti-D and diluted polyclonal anti-D, were selected by sampling batches of 100 to 200 samples from the previous day’s collection. Anti-D reagents used on the Olympus PK 7200 are required to detect RBCs with the weak D phenotype which do not agglutinate at immediate spin (IS) when tested with polyclonal anti-D by manual tube methods. More than 95 percent of donors tested were Caucasian. Using tube tests with two different monoclonal blend anti-D reagents and one polyclonal anti-D typing reagent, the presence or absence of the D antigen was evaluated after the IS reading. Donors found negative or weakly positive (< 2+) at IS were further typed for weak D by the IAT. The weak D samples were RHD genotyped by allele-specific PCR. Of 1005 donors tested, 4 (0.4%) were classified as weak D by one or more anti-D reagents. Polyclonal anti-D reagent demonstrated weaker reactions when compared with the monoclonal blends. All weak D samples were found positive for exon 4, intron 4, and exon 10, a finding consistent with most D+ samples. The incidence of weak D found in this study is not significantly different from that found in earlier studies using polyclonal anti-D reagents. Immunohematology2005;21:152–4.

Incidence of weak D in blood donors typed as D positive by the Olympus PK 7200

Immunohematology

{"article-title":"Incidence of weak D in blood donors typed as D positive by the Olympus PK 7200"}

The incidence of weak D has been reported to be between 0.23 and 0.5 percent in Europe and 3.0 percent in the United States. All studies were performed before...

Incidence of weak D in blood donors typed as D positive by the Olympus PK 7200

Pubblicato online: 01 mag 2020

Pagine: 152 - 154

DOI: https://doi.org/10.21307/immunohematology-2019-411

Parole chiave
incidence of weak D, anti-D typing reagents

© 2005 C.M. Jenkins, et al., published by Sciendo

Incidence of weak D in blood donors typed as D positive by the Olympus PK 7200

Pubblicato online: 01 mag 2020

Pagine: 152 - 154

DOI: https://doi.org/10.21307/immunohematology-2019-411

Parole chiaveincidence of weak D, anti-D typing reagents

© 2005 C.M. Jenkins, et al., published by Sciendo

Parole chiave
incidence of weak D, anti-D typing reagents