<abstract xmlns="http://www.w3.org/1999/xhtml">The low-prevalence MNS blood group antigen TSEN is located at the junction of glycophorin A (GPA) to glycophorin B (GPB) in several hybrid glycophorin molecules. Extremely rare people have RBCs with a double dose of the TSEN antigen and have made an antibody to a high-prevalence MNS antigen. We report the first patient who is heterozygous for <italic>GYP.JL</italic> and <italic>Mk</italic>. During prenatal tests, an alloantibody to a high-prevalence antigen was detected in the serum of a 21-year-old Hispanic woman. The antibody detected an antigen resistant to treatment by papain, trypsin, α-chymotrypsin, or DTT. The antibody was strongly reactive by the IAT with all RBCs tested except those having the MkMk, GP.Hil/GP.Hil, or GP.JL/GP.JL phenotypes. The patient’s RBCs typed M+N–S+/–s–U+, En(a+/–), Hut–, Mi(a–), Mur–, Vw–, Wr(a–b–), and were TSEN+, MINY+. Reactivity with <italic>Glycine soja</italic> suggested that her RBCs had a decreased level of sialic acid. Immunoblotting showed the presence of monomer and dimer forms of a GP(A-B) hybrid and an absence of GPA and GPB. Sequencing of DNA and PCR-RFLP using the restriction enzyme <italic>Rsa</italic>I confirmed the presence of a hybrid <italic>GYP(A-B)</italic>. The patient’s antibody was determined to be anti-EnaFR. She is the first person reported with the GP.JL phenotype associated with a deletion of <italic>GYPA</italic> and <italic>GYPB</italic> in <italic>trans</italic> to <italic>GYP.JL. <bold>Immunohematology</bold></italic><bold>2007;23:146–9.</bold></abstract>

The low-prevalence MNS blood group antigen TSEN is located at the junction of glycophorin A (GPA) to glycophorin B (GPB) in several hybrid glycophorin molecules. Extremely rare people have RBCs with a double dose of the TSEN antigen and have made an antibody to a high-prevalence MNS antigen. We report the first patient who is heterozygous for GYP.JL and Mk. During prenatal tests, an alloantibody to a high-prevalence antigen was detected in the serum of a 21-year-old Hispanic woman. The antibody detected an antigen resistant to treatment by papain, trypsin, α-chymotrypsin, or DTT. The antibody was strongly reactive by the IAT with all RBCs tested except those having the MkMk, GP.Hil/GP.Hil, or GP.JL/GP.JL phenotypes. The patient’s RBCs typed M+N–S+/–s–U+, En(a+/–), Hut–, Mi(a–), Mur–, Vw–, Wr(a–b–), and were TSEN+, MINY+. Reactivity with Glycine soja suggested that her RBCs had a decreased level of sialic acid. Immunoblotting showed the presence of monomer and dimer forms of a GP(A-B) hybrid and an absence of GPA and GPB. Sequencing of DNA and PCR-RFLP using the restriction enzyme RsaI confirmed the presence of a hybrid GYP(A-B). The patient’s antibody was determined to be anti-EnaFR. She is the first person reported with the GP.JL phenotype associated with a deletion of GYPA and GYPB in trans to GYP.JL. Immunohematology2007;23:146–9.

An alloantibody to a high-prevalence MNS antigen in a person with a GP.JL/Mk phenotype

Immunohematology

An alloantibody to a high-prevalence MNS antigen in a person with a GP.JL/M phenotype

The low-prevalence MNS blood group antigen TSEN is located at the junction of glycophorin A (GPA) to glycophorin B (GPB) in several hybrid glycophorin...

An alloantibody to a high-prevalence MNS antigen in a person with a GP.JL/M^k phenotype

Pubblicato online: 01 apr 2020

Pagine: 146 - 149

DOI: https://doi.org/10.21307/immunohematology-2019-332

© 2007 J. Ratliff, et al., published by Sciendo