<abstract xmlns="http://www.w3.org/1999/xhtml">Because of the scarcity of anti-Hy and anti-Joa, hemagglutination typing for the Dombrock blood group system antigens, Hy and Joa, is not feasible. The molecular bases associated with these antigens have been determined, making it possible to distinguish <italic>HY</italic> and <italic>JO</italic> from wild-type <italic>DO.</italic> This provides a tool to predict the probable phenotype of patients and to screen for antigen-negative donors. PCR-RFLP assays and a microchip assay were used to determine the frequency of <italic>HY</italic> and <italic>JO</italic> alleles in donors from Brazil and New York. DNA from random Brazilian donors, 288 by PCR-RFLP and 599 by the bead array method (BeadChip, BioArray Solutions, Warren, NJ), was tested to determine 323G/T (<italic>HY</italic>+/<italic>HY</italic>–) and 350C>T (<italic>JO</italic>+/<italic>JO</italic>–) single-nucleotide polymorphisms. In New York, 27,226 donors who self-identified as being African American were tested by hemagglutination with anti-Gya. Nonreactive and weakly reactive samples were tested by PCR-RFLP for the same alleles as listed above. In Brazil, 30 (3.4%) of the samples were <italic>JO/DO</italic> and 13 (1.4%) were <italic>HY/DO.</italic> In New York, of the samples that had <italic>HY</italic> or <italic>JO</italic> alleles, 14 were homozygous <italic>HY/HY,</italic> 132 were heterozygous <italic>HY/DO,</italic> 13 were heterozygous <italic>HY/JO,</italic> 14 were heterozygous <italic>JO/DO,</italic> and 3 were homozygous <italic>JO/JO.</italic> These results show that in donors from Brazil, <italic>JO</italic> (30 alleles) is more than twice as prevalent as <italic>HY</italic> (13 alleles), whereas in donors from New York, <italic>HY</italic> (173 alleles) was more than five times more common than <italic>JO</italic> (33 alleles). <italic><bold>Immunohematology</bold></italic><bold> 2008;24:135–137.</bold></abstract>

Because of the scarcity of anti-Hy and anti-Joa, hemagglutination typing for the Dombrock blood group system antigens, Hy and Joa, is not feasible. The molecular bases associated with these antigens have been determined, making it possible to distinguish HY and JO from wild-type DO. This provides a tool to predict the probable phenotype of patients and to screen for antigen-negative donors. PCR-RFLP assays and a microchip assay were used to determine the frequency of HY and JO alleles in donors from Brazil and New York. DNA from random Brazilian donors, 288 by PCR-RFLP and 599 by the bead array method (BeadChip, BioArray Solutions, Warren, NJ), was tested to determine 323G/T (HY+/HY–) and 350C>T (JO+/JO–) single-nucleotide polymorphisms. In New York, 27,226 donors who self-identified as being African American were tested by hemagglutination with anti-Gya. Nonreactive and weakly reactive samples were tested by PCR-RFLP for the same alleles as listed above. In Brazil, 30 (3.4%) of the samples were JO/DO and 13 (1.4%) were HY/DO. In New York, of the samples that had HY or JO alleles, 14 were homozygous HY/HY, 132 were heterozygous HY/DO, 13 were heterozygous HY/JO, 14 were heterozygous JO/DO, and 3 were homozygous JO/JO. These results show that in donors from Brazil, JO (30 alleles) is more than twice as prevalent as HY (13 alleles), whereas in donors from New York, HY (173 alleles) was more than five times more common than JO (33 alleles). Immunohematology 2008;24:135–137.

Molecular studies of <italic>DO</italic> alleles reveal that <italic>JO</italic> is more prevalent than <italic>HY</italic> in Brazil, whereas <italic>HY</italic> is more prevalent in New York

Immunohematology

Molecular studies of  alleles reveal that  is more prevalent than  in Brazil, whereas  is more prevalent in New York

Because of the scarcity of anti-Hy and anti-Joa, hemagglutination typing for the Dombrock blood group system antigens, Hy and Joa, is not feasible. The...

Molecular studies of DO alleles reveal that JO is more prevalent than HY in Brazil, whereas HY is more prevalent in New York

Article Category: Original Report

Pubblicato online: 25 mar 2020

Pagine: 135 - 137

DOI: https://doi.org/10.21307/immunohematology-2019-287

Parole chiave
blood groups, DNA testing, Dombrock, molecular basis

© 2008 L. Castilho, et al., published by Sciendo