<abstract xmlns="http://www.w3.org/1999/xhtml"><p>The Rh blood group system is highly complex both in the number of discrete antigens and in the existence of partial antigens, especially D and e. Recently, several partial c antigens have been reported. Here we report findings on an African American man with sickle cell disease whose RBCs typed C+c+ and whose plasma contained anti-c. Hemagglutination tests, DNA extraction, PCR-RFLP, reticulocyte RNA isolation, RT-PCR cDNA analyses, cloning, and sequencing were performed by standard procedures. RBCs from the patient typed C+c+ but his plasma contained alloanti-c. DNA analyses showed the presence of <italic>RHCE*Ce</italic> in <italic>trans</italic> to <italic>RHCE*ceAR</italic> with <italic>RHD*D</italic> and <italic>RHD*Weak D Type 4.2.2</italic>. The amino acid changes on RhceAR are such that a C+c+ patient made alloanti-c. This case shows that RhceAR carries a partial c antigen and illustrates the value of DNA testing as an adjunct to hemagglutination to aid in antibody identification in unusual cases. <italic><bold>Immunohematology</bold></italic><bold>2010;26:57–59.</bold></p></abstract>

The Rh blood group system is highly complex both in the number of discrete antigens and in the existence of partial antigens, especially D and e. Recently, several partial c antigens have been reported. Here we report findings on an African American man with sickle cell disease whose RBCs typed C+c+ and whose plasma contained anti-c. Hemagglutination tests, DNA extraction, PCR-RFLP, reticulocyte RNA isolation, RT-PCR cDNA analyses, cloning, and sequencing were performed by standard procedures. RBCs from the patient typed C+c+ but his plasma contained alloanti-c. DNA analyses showed the presence of RHCE*Ce in trans to RHCE*ceAR with RHD*D and RHD*Weak D Type 4.2.2. The amino acid changes on RhceAR are such that a C+c+ patient made alloanti-c. This case shows that RhceAR carries a partial c antigen and illustrates the value of DNA testing as an adjunct to hemagglutination to aid in antibody identification in unusual cases. Immunohematology2010;26:57–59.

<italic>RHCE*ceAR</italic> encodes a partial c (RH4) antigen

Immunohematology

{"article-title":{"italic":"RHCE*ceAR","content":"encodes a partial c (RH4) antigen"}}

The Rh blood group system is highly complex both in the number of discrete antigens and in the existence of partial antigens, especially D and e. Recently,...

RHCE*ceAR encodes a partial c (RH4) antigen

Article Category: Original Report

Pubblicato online: 12 mar 2020

Pagine: 57 - 59

DOI: https://doi.org/10.21307/immunohematology-2019-203

© 2010 C. Halter Hipsky, et al., published by Sciendo