<abstract xmlns="http://www.w3.org/1999/xhtml"><sec id="j_sjph-2016-0018_s_379_w2aab3b8c13b1b7b1aab1c15b1Aa"><h3>Introduction</h3><p>According to the existing literature, a heterogeneous sequence type (ST) or clones of community-associated methicillin-resistant <italic>Staphylococcus aureus</italic> (CA-MRSA) circulate in Europe. In Europe, the European clone that belongs to sequence type ST80 is predominant.</p></sec><sec id="j_sjph-2016-0018_s_380_w2aab3b8c13b1b7b1aab1c15b2Aa"><h3>Methods</h3><p>The aim of the study was to investigate the phenotypic and genotypic characteristics and epidemiological data of CA-MRSA ST80 and its occurrence in Slovenia. We retrospectively analyzed those CA-MRSA isolates that were isolated during microbiological procedures in microbiological laboratories between 2006 and 2013. Only CA-MRSA isolates from the national collection of CA-MRSA strains that belonged to ST80 (European clone) were analyzed. We determined the Pantone-Valentine leukocidin (PVL), <italic>mec</italic> A genes, exfoliative toxin genes and type of staphylococcal cassette chromosome (SCC<italic>mec</italic>) by polymerase chain reaction (PCR). We determined also spa type and sequence type.</p></sec><sec id="j_sjph-2016-0018_s_382_w2aab3b8c13b1b7b1aab1c15b3Aa"><h3>Results</h3><p>ST80 was confirmed in only 2 (0.5%) out of 385 CA-MRSA isolates, collected in a national collection of CAMRSA. Both isolates were positive for the PVL genes, <italic>mec</italic> A gene, exfoliative toxin type D gene and SCC<italic>mec</italic> IV. One CA-MRSA isolate was confirmed in a wound swab taken from a 47-year-old male, and the second was isolated from blood cultures of a 69-year-old female. No epidemiological connections between them were found.</p></sec><sec id="j_sjph-2016-0018_s_383_w2aab3b8c13b1b7b1aab1c15b4Aa"><h3>Conclusions</h3><p>In Slovenia CA-MRSA infections caused by ST80 are rare. In the future, it is necessary that a surveillance study of CA-MRSA at the national level continues and CA-MRSA be considered as a public health threat.</p></sec></abstract>

IntroductionAccording to the existing literature, a heterogeneous sequence type (ST) or clones of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) circulate in Europe. In Europe, the European clone that belongs to sequence type ST80 is predominant.MethodsThe aim of the study was to investigate the phenotypic and genotypic characteristics and epidemiological data of CA-MRSA ST80 and its occurrence in Slovenia. We retrospectively analyzed those CA-MRSA isolates that were isolated during microbiological procedures in microbiological laboratories between 2006 and 2013. Only CA-MRSA isolates from the national collection of CA-MRSA strains that belonged to ST80 (European clone) were analyzed. We determined the Pantone-Valentine leukocidin (PVL), mec A genes, exfoliative toxin genes and type of staphylococcal cassette chromosome (SCCmec) by polymerase chain reaction (PCR). We determined also spa type and sequence type.ResultsST80 was confirmed in only 2 (0.5%) out of 385 CA-MRSA isolates, collected in a national collection of CAMRSA. Both isolates were positive for the PVL genes, mec A gene, exfoliative toxin type D gene and SCCmec IV. One CA-MRSA isolate was confirmed in a wound swab taken from a 47-year-old male, and the second was isolated from blood cultures of a 69-year-old female. No epidemiological connections between them were found.ConclusionsIn Slovenia CA-MRSA infections caused by ST80 are rare. In the future, it is necessary that a surveillance study of CA-MRSA at the national level continues and CA-MRSA be considered as a public health threat.

Infections Caused By Community-Associated Methicillin-Resistant <italic>Staphylococcus Aureus</italic> European Clone (ST80) In Slovenia Between 2006 And 2013

National Laboratory for Health, Environment and Food (NLZOH), Department of Medical Microbiology, Gosposvetska ulica 12, 4000 Kranj,

University Medical Centre Ljubljana, Department of Infectious Diseases, Japljeva 2, 1000 Ljubljana,

National Laboratory for Health, Environment and Food (NLZOH), Department of Medical Microbiology, Mej vrti 5, 8000 Novo mesto,

National Institute of Public Health (NIJZ), Regional Unit Novo mesto, Mej vrti 5, 8000 Novo mesto,

Institute of Microbiology and Parasitology, University of Ljubljana, Veterinary Faculty, Gerbičeva 60, 1000 Ljubljana,

Slovenian Journal of Public Health

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Infections Caused By Community-Associated Methicillin-Resistant  European Clone (ST80) In Slovenia Between 2006 And 2013

IntroductionAccording to the existing literature, a heterogeneous sequence type (ST) or clones of community-associated methicillin-resistant Staphylococcus...

Characteristic
Gender	M	F
Age (years)	47	69
Diagnosis at hospital admission	Abscessus glutei	Meningeoma
Source of isolates	Wound swab	Blood cultures, tracheal aspirate
Type of infection	SSTI	Sepsis and meningitis
Outcome	recovered	death
Risk factors for MRSA colonization
History of MRSA infection / colonization	No	No
Surgery in the past year	No	Yes
Hospitalization in the past year	No	Yes
Residence in a day care	No	No
Resistance pattern	P, OX, TE, FA, K	P, OX, TE, FA, K
MIC of oxacillin (mg/L)	16	64
Typing
SCCmec type	IV	IV
spa type	t044	t044
MLST (ST)	80	80
Toxin gene profile
PVL	+	+
etd	+	+

Infections Caused By Community-Associated Methicillin-Resistant Staphylococcus Aureus European Clone (ST80) In Slovenia Between 2006 And 2013

Article Category: Original scientific article

Pubblicato online: 11 feb 2016

Pagine: 131 - 135

Ricevuto: 09 set 2015

Accettato: 09 dic 2015

DOI: https://doi.org/10.1515/sjph-2016-0018

Parole chiave
CA-MRSA, ST80 (European clone), invasive infections, death, Slovenia

© National Institute of Public Health, Slovenia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypical, genotypical and epidemiological characteristics and clinical data from patients infected with CA-MRSA ST80 strain in Slovenia between 2006 and 2013.

Infections Caused By Community-Associated Methicillin-Resistant Staphylococcus Aureus European Clone (ST80) In Slovenia Between 2006 And 2013

Article Category: Original scientific article

Pubblicato online: 11 feb 2016

Pagine: 131 - 135

Ricevuto: 09 set 2015

Accettato: 09 dic 2015

DOI: https://doi.org/10.1515/sjph-2016-0018

Parole chiaveCA-MRSA, ST80 (European clone), invasive infections, death, Slovenia

© National Institute of Public Health, Slovenia

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Phenotypical, genotypical and epidemiological characteristics and clinical data from patients infected with CA-MRSA ST80 strain in Slovenia between 2006 and 2013.

Parole chiave
CA-MRSA, ST80 (European clone), invasive infections, death, Slovenia