Protective/restorative role of the adipose tissue-derived mesenchymal stem cells on the radioiodine-induced salivary gland damage in rats

To analyze protective/regenerative effects of adipose tissue-derived mesenchymal stem cells (ADMSC) on ¹³¹I-Radioiodine (RAI)-induced salivary gland damage in rats.

Materials and Methods

Study population consisted of controls (n:6) and study groups (n:54): RAI (Group 1), ADMSC (Group 2), amifostine (Group 3), RAI+amifostine (Group 4), concomitant RAI+ADMSC (Group 5) and RAI+ADMSC after 48 h (Group 6). We used light microscopy (LM), transmission electron microscopy (TEM), and salivary gland scintigraphy (SGS), and analyzed data statistically.

Results

We observed the homing of ADMSC in salivary glands at 1^st month on LM. RAI exposure affected necrosis, periductal fibrosis, periductal sclerosis, vascular sclerosis and the total sum score were in a statistically significant manner (P < 0.05). Intragroup comparisons with LM at 1^st and 6^th months revealed statistically significant improvements in Group 6 (P < 0.05) but not in Groups 4 and 5. Intergroup comparisons of the total score showed that Groups 4 and 5 in 1^st month and Group 6 in 6^th month had the lowest values. TEM showed vacuolization, edema, and fibrosis at 1^st month, and an improvement in damage in 6^th month in Groups 5 and 6. SGSs revealed significant differences for the maximum secretion ratio (Smax) (P = 0.01) and the gland-to-background ratio at a maximum count (G/BGmax) (P = 0. 01) at 1^st month, for G/BGmax (P = 0.01), Smax (P = 0.01) and the time to reach the maximum count ratio over the time to reach the minimum count (Tmax/Tmin) (P = 0.03) at 6^th month. 1^st and 6^th month scans showed differences for Smax and G/BGmax (P = 0.04), but not for Tmax/Tmin (p > 0.05). We observed a significant deterioration in gland function in group 1, whereas, mild to moderate deteriorations were seen in protective treatment groups.

Conclusions

Our results indicated that ADMSC might play a promising role as a protective/regenerative agent against RAI-induced salivary gland dysfunction.

Lingua:: Inglese

Frequenza di pubblicazione:: 4 volte all'anno
Argomenti della rivista:: Medicina, Medicina clinica, Medicina interna, Ematologia, Oncologia, Radiologia

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Protective/restorative role of the adipose tissue-derived mesenchymal stem cells on the radioiodine-induced salivary gland damage in rats

Güleser Saylam

Ömer Bayır

Salih Sinan Gültekin

Ferda Alparslan Pınarlı

Ünsal Han

Mehmet Hakan Korkmaz

Mehmet Eser Sancaktar

İlkan Tatar

Mustafa Fevzi Sargon

Emel Çadallı Tatar

Categoria dell'articolo: Research Article

Pubblicato online: 14 giu 2017

Pagine: 307 - 316

Ricevuto: 21 dic 2016

Accettato: 07 apr 2017

DOI: https://doi.org/10.1515/raon-2017-0022

Parole chiavesalivary gland, radioiodine treatment, radiation damage, radiation protection, stem cell transplantation, adipose tissue-derived mesenchymal stem cells

© 2017 Güleser Saylam, Ömer Bayır, Salih Sinan Gültekin, Ferda Alparslan Pınarlı, Ünsal Han, Mehmet Hakan Korkmaz, Mehmet Eser Sancaktar, İlkan Tatar, Mustafa Fevzi Sargon, Emel Çadallı Tatar

This work is licensed under the Creative Commons Attribution 4.0 International License.

Background

Materials and Methods

Results

Conclusions

Parole chiave
salivary gland, radioiodine treatment, radiation damage, radiation protection, stem cell transplantation, adipose tissue-derived mesenchymal stem cells