<abstract xmlns="http://www.w3.org/1999/xhtml"><p>To understand the mechanisms standing behind a successful stem cell-based therapy, the monitoring of transplanted cell’s migration, homing as well as the engraftment efficiency and functional capability <italic>in-vivo</italic> has become a critical issue. The present study was designed to track the labelled oADMSCs <italic>in-vitro</italic> and its visualization through MRI technique. oADMSCs from passage 4 (P-4) to passage 6 (P-6) were labelled with superparamagnetic iron oxide (SPIO) conjugated with rhodamine (Molday Ion Rhodamine-B - MIRB) at the concentration of 25μg Fe/ml in DMEM. Internalized MIRB was observed under fluorescent microscope after 72 hrs of incubation. Labelled oADMSCs showed Prussian Blue positive reaction demonstrating the iron uptake of the cells. The viability of the MIRB-labelled oADMSCs ranged between 98-99 per cent and Trypan blue exclusion test showed no significant difference in viability between labelled and unlabelled oADMSCs. MR signal in control group of cells was similar to that of water. MR signals or fluorescence in MIRB-labelled cells decreased with increasing concentrations of iron. The T2 weighted images of MIRB-labelled oADMSCs increased with increasing concentrations of SPIOs. The MIRB was found to be nontoxic, and did not affect proliferation capacity <italic>in-vitro.</italic></p></abstract>

To understand the mechanisms standing behind a successful stem cell-based therapy, the monitoring of transplanted cell’s migration, homing as well as the engraftment efficiency and functional capability in-vivo has become a critical issue. The present study was designed to track the labelled oADMSCs in-vitro and its visualization through MRI technique. oADMSCs from passage 4 (P-4) to passage 6 (P-6) were labelled with superparamagnetic iron oxide (SPIO) conjugated with rhodamine (Molday Ion Rhodamine-B - MIRB) at the concentration of 25μg Fe/ml in DMEM. Internalized MIRB was observed under fluorescent microscope after 72 hrs of incubation. Labelled oADMSCs showed Prussian Blue positive reaction demonstrating the iron uptake of the cells. The viability of the MIRB-labelled oADMSCs ranged between 98-99 per cent and Trypan blue exclusion test showed no significant difference in viability between labelled and unlabelled oADMSCs. MR signal in control group of cells was similar to that of water. MR signals or fluorescence in MIRB-labelled cells decreased with increasing concentrations of iron. The T2 weighted images of MIRB-labelled oADMSCs increased with increasing concentrations of SPIOs. The MIRB was found to be nontoxic, and did not affect proliferation capacity in-vitro.

<italic>In-vitro</italic> Labelling of Ovine Adipose-Derived Mesenchymal Stem Cells (oADMSCs) and Tracking Using MRI Technique

Department of Veterinary Anatomy, Madras Veterinary College

Tamil Nadu Veterinary and Animal Sciences University

Centre for Stem Cell Research and Regenerative Medicine, Madras Veterinary College

Department of Animal Biotechnology, Madras Veterinary College

Macedonian Veterinary Review

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

 Labelling of Ovine Adipose-Derived Mesenchymal Stem Cells (oADMSCs) and Tracking Using MRI Technique

To understand the mechanisms standing behind a successful stem cell-based therapy, the monitoring of transplanted cell’s migration, homing as well as the...

In-vitro Labelling of Ovine Adipose-Derived Mesenchymal Stem Cells (oADMSCs) and Tracking Using MRI Technique

Pubblicato online: 17 ott 2017

Pagine: 137 - 142

Ricevuto: 27 nov 2016

Accettato: 15 apr 2017

DOI: https://doi.org/10.1515/macvetrev-2017-0018

Parole chiave
ovine, mesenchymal stem cells, in-vitro MIRB labelling, MRI imaging

© 2017 Ravi Gnanam Gnanadevi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

In-vitro Labelling of Ovine Adipose-Derived Mesenchymal Stem Cells (oADMSCs) and Tracking Using MRI Technique

Pubblicato online: 17 ott 2017

Pagine: 137 - 142

Ricevuto: 27 nov 2016

Accettato: 15 apr 2017

DOI: https://doi.org/10.1515/macvetrev-2017-0018

Parole chiaveovine, mesenchymal stem cells, in-vitro MIRB labelling, MRI imaging

© 2017 Ravi Gnanam Gnanadevi et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
ovine, mesenchymal stem cells, in-vitro MIRB labelling, MRI imaging