<abstract xmlns="http://www.w3.org/1999/xhtml">Propylthiouracil is used against Grave's Disease and for developing animal models of hypothyroidism. Apart from depressed metabolism, free radical-induced tissue damage has been registered as an effect from this drug. Superoxide is essential for generation of free radicals in tissues. The mutual effects of Propylthiouracil and superoxide radical have not been well investigated.The <italic>in vitro</italic> effects of Propylthiouracil on the free radicals in three model systems generating superoxide were measured using luminol-dependent chemiluminescence and spectrophotometry. The drug did not affect the formation of free radicals in the presence of potassium superoxide and in pyrogallol-oxygen solutions, while in the presence of the xanthine/xanthine oxidase system a distinct prooxidant effect was registered. The investigation of the system propylthiouracil/xanthine oxidase showed mild free radicals formation along with decreasing intensities of the drug's UV-specter.Our <italic>in vitro</italic> investigation indicated that, along with the transformation of xanthine to uric acid over xanthine oxidase, some free radicals may be produced due to the interaction of propylthiouracil with the enzyme. It was proposed that this might contribute to the <italic>in vivo</italic> free radicals-induced tissue damage observed in the presence of propylthiouracil.</abstract>

Propylthiouracil is used against Grave's Disease and for developing animal models of hypothyroidism. Apart from depressed metabolism, free radical-induced tissue damage has been registered as an effect from this drug. Superoxide is essential for generation of free radicals in tissues. The mutual effects of Propylthiouracil and superoxide radical have not been well investigated.The in vitro effects of Propylthiouracil on the free radicals in three model systems generating superoxide were measured using luminol-dependent chemiluminescence and spectrophotometry. The drug did not affect the formation of free radicals in the presence of potassium superoxide and in pyrogallol-oxygen solutions, while in the presence of the xanthine/xanthine oxidase system a distinct prooxidant effect was registered. The investigation of the system propylthiouracil/xanthine oxidase showed mild free radicals formation along with decreasing intensities of the drug's UV-specter.Our in vitro investigation indicated that, along with the transformation of xanthine to uric acid over xanthine oxidase, some free radicals may be produced due to the interaction of propylthiouracil with the enzyme. It was proposed that this might contribute to the in vivo free radicals-induced tissue damage observed in the presence of propylthiouracil.

Interaction Of Propylthiouracil With Model Systems Generating A Superoxide Radical

Department of Pharmacology and Toxicology, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria

Department of Medical Physics and Biophysics, Medical Faculty, Medical University of Sofia, Sofia, Bulgaria

Journal of Biomedical and Clinical Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Propylthiouracil is used against Grave's Disease and for developing animal models of hypothyroidism. Apart from depressed metabolism, free radical-induced...

Interaction Of Propylthiouracil With Model Systems Generating A Superoxide Radical

Pubblicato online: 10 dic 2015

Pagine: 93 - 98

Ricevuto: 19 ott 2014

Accettato: 19 dic 2014

DOI: https://doi.org/10.1515/jbcr-2015-0132

Parole chiave
propylthiouracil, xanthine oxidase, superoxide radical, free radicals formation

© 2014 Maria L. Valcheva-Traykova et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Interaction Of Propylthiouracil With Model Systems Generating A Superoxide Radical

Pubblicato online: 10 dic 2015

Pagine: 93 - 98

Ricevuto: 19 ott 2014

Accettato: 19 dic 2014

DOI: https://doi.org/10.1515/jbcr-2015-0132

Parole chiavepropylthiouracil, xanthine oxidase, superoxide radical, free radicals formation

© 2014 Maria L. Valcheva-Traykova et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
propylthiouracil, xanthine oxidase, superoxide radical, free radicals formation