<abstract xmlns="http://www.w3.org/1999/xhtml"><p>A total of 102 honey samples collected from small apiaries (≤ 20 hives) in Poland were analysed for the presence of <italic>Clostridium botulinum</italic> spores. The samples were prepared using the dilution centrifugation method and cultured in parallel in cooked meat medium (CMM) and tripticase peptone glucose yeast (TPGY) enrichment broths. Identification of toxin types A, B, and E of <italic>Clostridium botulinum</italic> strains was performed with the use of the multiplex PCR method. Positive samples were also subjected to quantitative analysis with the use of <italic>Clostridium botulinum</italic> Isolation Agar Base (CBAB). The prevalence analysis showed 22 (21.6%) samples contaminated with <italic>C. botulinum</italic> spores. The major serotype detected was botulin neurotoxin type A – 16 (72.7%) whereas type B was found in 3 (13.6%) honey samples and type E also only in 3 (13.6%) honey samples. Dual-toxin-producing strains were noted. The average quantity of spores in PCR - <italic>C. botulinum</italic> positive samples was 190 in 1 gram of honey.</p></abstract>

A total of 102 honey samples collected from small apiaries (≤ 20 hives) in Poland were analysed for the presence of Clostridium botulinum spores. The samples were prepared using the dilution centrifugation method and cultured in parallel in cooked meat medium (CMM) and tripticase peptone glucose yeast (TPGY) enrichment broths. Identification of toxin types A, B, and E of Clostridium botulinum strains was performed with the use of the multiplex PCR method. Positive samples were also subjected to quantitative analysis with the use of Clostridium botulinum Isolation Agar Base (CBAB). The prevalence analysis showed 22 (21.6%) samples contaminated with C. botulinum spores. The major serotype detected was botulin neurotoxin type A – 16 (72.7%) whereas type B was found in 3 (13.6%) honey samples and type E also only in 3 (13.6%) honey samples. Dual-toxin-producing strains were noted. The average quantity of spores in PCR - C. botulinum positive samples was 190 in 1 gram of honey.

<italic>Clostridium botulinum</italic> Spores Found in Honey from Small Apiaries in Poland

Chair of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn,

Department of Hormonal Action Mechanisms, Institute of Animal Reproduction and Food Research, the Polish Academy of Sciences

Research Institute of Horticulture, Apiculture Division in Puławy,

Journal of Apicultural Science

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 Spores Found in Honey from Small Apiaries in Poland

A total of 102 honey samples collected from small apiaries (≤ 20 hives) in Poland were analysed for the presence of Clostridium botulinum spores. The samples...

Clostridium botulinum Spores Found in Honey from Small Apiaries in Poland

Pubblicato online: 08 dic 2016

Pagine: 89 - 100

Ricevuto: 15 feb 2016

Accettato: 26 mag 2016

DOI: https://doi.org/10.1515/jas-2016-0020

Parole chiave
honey, PCR, Poland, spores

© 2016 Joanna Wojtacka et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Clostridium botulinum Spores Found in Honey from Small Apiaries in Poland

Pubblicato online: 08 dic 2016

Pagine: 89 - 100

Ricevuto: 15 feb 2016

Accettato: 26 mag 2016

DOI: https://doi.org/10.1515/jas-2016-0020

Parole chiavehoney, PCR, Poland, spores

© 2016 Joanna Wojtacka et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
honey, PCR, Poland, spores