<abstract xmlns="http://www.w3.org/1999/xhtml"><p>To investigate susceptibility of young Scots pine seedlings to four <italic>Phytophthora</italic> species: <italic>Phytophthora cactorum</italic>, <italic>Phytophthora cambivora</italic>, <italic>Phytophthora plurivora</italic> and <italic>Phytophthora pini</italic>; seven-day-old seedlings of Scots pine (15 seedlings per experiment) were infected using agar plugs of the respective species. Control group also consisted of 15 seedlings and was inoculated with sterile agar plugs. Results unambiguously show that after 4.5 days, all seedlings show clear signs of infection and display severe symptoms of tissue damage and necrosis. Moreover, three and two seedlings in the <italic>P. cactorum</italic> and <italic>P. cambivora</italic> infected seedlings groups, respectively, collapsed. The length of largest necrosis measured 13.4±3.90 mm and was caused by <italic>P. cactorum</italic>. To rule out any putative contamination or infection by secondary pathogens, re-isolations of pathogens from infection sites were performed and were positive in 100% of plated pieces of infected seedlings. All re-isolations were, however, negative in the case of the control group. Detailed microscopic analyses of infected tissues of young seedlings confirmed the presence of numerous <italic>Phytophthora</italic> species inside and on the surface of infected seedlings. Therefore, our results suggest <italic>Phytophthora</italic> spp. and mainly <italic>P. cactorum</italic> and <italic>P. cambivora</italic> as aggressive pathogens of Scots pine seedlings and highlight a putative involvement of these species in the damping off of young Scots pine seedlings frequently observed in forest nurseries.</p></abstract>

To investigate susceptibility of young Scots pine seedlings to four Phytophthora species: Phytophthora cactorum, Phytophthora cambivora, Phytophthora plurivora and Phytophthora pini; seven-day-old seedlings of Scots pine (15 seedlings per experiment) were infected using agar plugs of the respective species. Control group also consisted of 15 seedlings and was inoculated with sterile agar plugs. Results unambiguously show that after 4.5 days, all seedlings show clear signs of infection and display severe symptoms of tissue damage and necrosis. Moreover, three and two seedlings in the P. cactorum and P. cambivora infected seedlings groups, respectively, collapsed. The length of largest necrosis measured 13.4±3.90 mm and was caused by P. cactorum. To rule out any putative contamination or infection by secondary pathogens, re-isolations of pathogens from infection sites were performed and were positive in 100% of plated pieces of infected seedlings. All re-isolations were, however, negative in the case of the control group. Detailed microscopic analyses of infected tissues of young seedlings confirmed the presence of numerous Phytophthora species inside and on the surface of infected seedlings. Therefore, our results suggest Phytophthora spp. and mainly P. cactorum and P. cambivora as aggressive pathogens of Scots pine seedlings and highlight a putative involvement of these species in the damping off of young Scots pine seedlings frequently observed in forest nurseries.

Four different <italic>Phytophthora</italic> species that are able to infect Scots pine seedlings in laboratory conditions

Forest Research Institute, Department of Forest Protection, Braci Leśnej 3, 05-090 Raszyn,

Forest Research Institute, Laboratory of Molecular Biology, Braci Leśnej 3, 05-090 Raszyn,

Forest Research Institute, Department of Silviculture and Genetics of Forest Trees, Braci Leśnej 3, 05-090 Raszyn,

Technical University in Białystok, Forest Faculty, Piłsudskiego 8, 17-200 Hajnówka,

University of Neuchatel, Laboratory of Soil Biology, Rue Emile Argand 11, 2009 Neuchâtel,

Institute of Forestry Belgrade, Kneza Višeslava 3, 11030 Belgrade,

Folia Forestalia Polonica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Four different  species that are able to infect Scots pine seedlings in laboratory conditions

To investigate susceptibility of young Scots pine seedlings to four Phytophthora species: Phytophthora cactorum, Phytophthora cambivora, Phytophthora plurivora...

Four different Phytophthora species that are able to infect Scots pine seedlings in laboratory conditions

Pubblicato online: 08 ott 2016

Pagine: 123 - 130

DOI: https://doi.org/10.1515/ffp-2016-0014

Parole chiave
pathogenicity, light microscopy

© 2016 Miłosz Tkaczyk et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Four different Phytophthora species that are able to infect Scots pine seedlings in laboratory conditions

Pubblicato online: 08 ott 2016

Pagine: 123 - 130

DOI: https://doi.org/10.1515/ffp-2016-0014

Parole chiavepathogenicity, light microscopy

© 2016 Miłosz Tkaczyk et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
pathogenicity, light microscopy