The study was performed in five fourth-order tributaries of the Bystrzyca Lubelska River (Eastern Poland, Lublin Upland), differing in the degree of river-bed transformation and level of pollution. Hydro-morphological methods (descriptive method by Ilnicki and Lewandowski - IL, and index method by Oglecki and Pawlat - OP) and biological indices based on the composition of zoobenthos (Diversity -