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Associations of biochemical changes and maternal traits with mutation 1843 (C>T) in the RYR1 gene as a common cause for porcine stress syndrome

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Thirteen percent polyacrylamide gel electrophoresis (PAGE) for PSS genotyping of the digested fragment of interest from the ryr1 gene was done by RFLP using the HhaI restriction enzyme.Lane 1: heterozygous (Nn genotype);lane 2: homozygous (nn genotype); L: low range DNA ladder;lane 3: normal (NN genotype).
Thirteen percent polyacrylamide gel electrophoresis (PAGE) for PSS genotyping of the digested fragment of interest from the ryr1 gene was done by RFLP using the HhaI restriction enzyme.Lane 1: heterozygous (Nn genotype);lane 2: homozygous (nn genotype); L: low range DNA ladder;lane 3: normal (NN genotype).

Biochemical data of plasma samples from animals with different porcine stress syndrome genotypes.

Biochemical ParameterGenotypeReference Values

Reference values from the Research Animal Resources, University of Minnesota, Minneapolis, MN, USA (www.ahc.umn.edu/rar/refvalues.htm).

t Value
NNNnnn
Ca2+ (µmol/L)2.622.682.712.50-2.951.14

A p value of >0.05 was not considered to be significant.

Creatinine (µmol/L)142.10157.30174.6088.40-265.209.97

A p value of <0.05 was considered to be significant.

Total proteins (g/L)72.4075.7074.9068.00-89.001.42

A p value of >0.05 was not considered to be significant.

Bilirubin (µmol/L)1.652.544.120.00-12.0012.79

A p value of <0.05 was considered to be significant.

AP (U/L)21.8045.3062.409.00-70.0014.55

A p value of <0.05 was considered to be significant.

AST (U/L)29.2058.30125.5017.00-45.0016.32

A p value of <0.05 was considered to be significant.

ALT (U/L)35.3051.7048.209.00-60.009.14

A p value of >0.05 was not considered to be significant.

LDH (U/L398.00655.00894.00159.00-475.0017.02

A p value of <0.05 was considered to be significant.

CPK (U/L)276.00591.00764.0067.00-489.0015.29

A p value of <0.05 was considered to be significant.

Association of the porcine stress syndrome genotype with maternal traits (newborn, stillborn and weaned piglets).

PSS GenotypeNewborn Pigletst Value
NN10.920.22
Nn11.730.522.59

A p value of <0.05 was considered to be significant.

nn11.501.50
Stillborn Piglets
NN0.150.06
Nn0.090.092.53

A p value of <0.05 was considered to be significant.

nn1.50.50
Weaned Piglets
NN8.200.19
Nn9.000.460.05

A p value of >0.05 was not considered to be significant.

nn7.500.50
eISSN:
1311-0160
Lingua:
Inglese
Frequenza di pubblicazione:
2 volte all'anno
Argomenti della rivista:
Medicine, Basic Medical Science, other