<abstract xmlns="http://www.w3.org/1999/xhtml"> Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient.</abstract>

Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides and glycoproteins. The aim of the study was to evaluate the protein content in dried roots of Echinacea purpurea (L.) Moench after homogenization of roots with liquid nitrogen, extraction in 0.01 mol L-1 phosphate-buffered saline (PBS) and purification followed by fractionation of proteins using gel filtration chromatography. Total concentration of proteins was measured using the Bradford method, and evaluation of the molecular mass of proteins was accomplished by applying the SDS-PAGE gel electrophoresis. The Bradford assay revealed that the highest concentration of proteins in fractions collected after gel filtration chomatography was 4.66–6.07 mg mL-1. Glycoproteins, alkamides and polysaccharides in roots of Echinacea purpurea (L.) Moench are chemical compounds that are responsible for their immunomodulatory properties. However, information about the difference of protein contents in fresh and dried roots of E. purpurea is insufficient.

Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench

Department of Pharmacognosy Lithuanian University of Health Sciences Kaunas, Lithuania

Department of Social Pharmacy and Drug Technology, Lithuanian University of Health Sciences, Kaunas, Lithuania 4Kaunas Botanical Garden of Vytautas Magnus University Kaunas, Lithuania

Kaunas Botanical Garden of Vytautas Magnus University Kaunas, Lithuania

Institute of Biotechnology Vilnius University, Vilnius, Lithuania

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Echinacea purpurea (L.) Moench, a member of the Asteraceae family, is a plant rich in flavonoids, essential oils, phenolic compounds, saponins, polysaccharides...

Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench

Pubblicato online: 17 dic 2015

Pagine: 473 - 479

Accettato: 14 lug 2015

DOI: https://doi.org/10.1515/acph-2015-0036

Parole chiave
Echinacea purpurea (L.) Moench, proteins, gel filtration chromatography, SDS-PAGE

© by Nijole Savickiene

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Fractionation and evaluation of proteins in roots of Echinacea purpurea (L.) Moench

Pubblicato online: 17 dic 2015

Pagine: 473 - 479

Accettato: 14 lug 2015

DOI: https://doi.org/10.1515/acph-2015-0036

Parole chiaveEchinacea purpurea (L.) Moench, proteins, gel filtration chromatography, SDS-PAGE

© by Nijole Savickiene

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Parole chiave
Echinacea purpurea (L.) Moench, proteins, gel filtration chromatography, SDS-PAGE