<abstract xmlns="http://www.w3.org/1999/xhtml"><p> The effect of the alkaloid 1-(phenyl)-N’-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU). Both methodologies suggested that the mode of action was avoidance of infection progression in the host cell. The compound was able to prevent CPE and PFU formation when the cells were pretreated with PMC for 24 h prior to PV infection. In addition, when the alkaloid was continuously maintained in the infected cultures, the spread of PV to adjacent cells was impaired. The pre-exposure and post-exposure prophylactic applications are possible situations in which an anti-PV drug might be used. Future studies are needed to elucidate the PMC mode of action and verify the feasibility of PMC use in vivo. No antipicornavirus agent is currently approved for clinical use</p></abstract>

The effect of the alkaloid 1-(phenyl)-N’-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero cells was assayed by inhibition of the cytopathic effect (CPE) and inhibition of plaque forming units (PFU). Both methodologies suggested that the mode of action was avoidance of infection progression in the host cell. The compound was able to prevent CPE and PFU formation when the cells were pretreated with PMC for 24 h prior to PV infection. In addition, when the alkaloid was continuously maintained in the infected cultures, the spread of PV to adjacent cells was impaired. The pre-exposure and post-exposure prophylactic applications are possible situations in which an anti-PV drug might be used. Future studies are needed to elucidate the PMC mode of action and verify the feasibility of PMC use in vivo. No antipicornavirus agent is currently approved for clinical use

Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication

Post-Graduate Program in Pharmaceutical Sciences State University of Maringá 87020-900, Maringá, PR, Brazil

Post-Graduate Program in Chemistry State University of Maringá 87020-900, Maringá, PR, Brazil

Acta Pharmaceutica

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

The effect of the alkaloid 1-(phenyl)-N’-(4-methoxybenzylidene)- 9H-pyrido[3,4-b]indole-3-carbohydrazide (PMC) on the poliovirus (PV) replication cycle in Vero...

Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication

Pubblicato online: 11 mar 2015

Pagine: 75 - 81

Ricevuto: 12 ott 2014

DOI: https://doi.org/10.1515/acph-2015-0004

Parole chiave
1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[ 3,4-b]indole-3-carbohydrazide, b-carboline, poliovirus, antiviral activity

© by Tania Ueda-Nakamura

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Effect of 1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[3,4-b] indole-3-carbohydrazide on in vitro poliovirus replication

Pubblicato online: 11 mar 2015

Pagine: 75 - 81

Ricevuto: 12 ott 2014

DOI: https://doi.org/10.1515/acph-2015-0004

Parole chiave1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[ 3,4-b]indole-3-carbohydrazide, b-carboline, poliovirus, antiviral activity

© by Tania Ueda-Nakamura

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parole chiave
1-(phenyl)-N’-(4-methoxybenzylidene)-9H-pyrido[ 3,4-b]indole-3-carbohydrazide, b-carboline, poliovirus, antiviral activity