<abstract xmlns="http://www.w3.org/1999/xhtml"> Objectives: There are many treatment protocols used these days, but seems like no one ensures a hundred percent effectiveness for lifelong prosthesis function. The aim of the study was to investigate whether some new method could mitigate calcification better than usually used and to compare impact on different valve tissues.Methods: Porcine aortic and mitral valve cusps were treated with glutaraldehyde and carbodiimide. Control group without any treatment was also used. Cusp pieces were implanted subcutaneously into 90 rats for 60 days. Tissue calcification was assessed by atomic absorption spectrophotometry.Results: The most calcification was found when not used any treatment, and the lowest calcium level was in group when treated with carbodiimide. Glutaraldehyde in low concentration lowered significantly residual calcium level, but not so effectively as carbodiimide. All data differ statistically significantly (p &lt; 0.05).Conclusion: Calcification was minimal treating porcine valve tissue with carbodiimide comparing to glutaraldehyde or no treatment.</abstract>

Department of Cardiovascular Diseases, Vilnius University, Vilnius, Lithuania

Department of Cardiac Surgery, Seamen’s Hospital, Liepojos 45, LT-92288, Klaipeda, Lithuania. Tel.: +370 650 12900; Fax: +370 46385338

Faculty of Chemistry, Vilnius University, Vilnius, Lithuania

Impact on porcine heart valve tissue treating with carbodiimide

<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: There has been a continued search for an accurate noninvasive technique for detecting subclinical acute rejection in heart transplant recipients. Ultrasonic deformation imaging - strain/strain rate (SR) - is sensitive in detecting sub-clinical abnormalities in regional myocardial function and could potentially be sensitive tool to detect changes in deformation induced by graft rejection. There is an evidence of the importance of immunophenotyping in determining transplant rejection as well.Aim: to assess the potential role of cardiac ultrasound velocity/strain imaging and immunological testing (alterations in peripheral blood T-cells subsets activation) in the detection of acute allograft rejection proven by endomyocardial biopsy.Patients and methods: A retrospective observational study was carried out involving 28 patients (22 men and 6 women) who underwent a total of 167 routine follow up endomyocardial biopsies with correlative cardiac ultrasound and immunophenotyping data. Myocardial velocity derived from pulsed wave tissue Doppler imaging (PW-TDI) was calculated in the longitudinal direction in basal lateral segment of left ventricle (LV) in 4-chamber view and in the radial direction in basal posterior LV segment in long parasternal axis view. Global systolic strain by speckle tracking was calculated in the longitudinal, radial and circumferential directions.Results: According to the International Society of Heart and Lung Transplantation criteria, 90 biopsies (Group 1) had grade 0, 1R or 2R rejection, and 30 biopsies (Group 2) had grade 3R rejection. The results of the forward selection revealed that the best indicator to predict the rejection was the amount of CD4+/HLA-DR+ cells. Univariate logistic regression analysis showed that global radial systolic strain performs better in terms of receiver-operator-characteristic curves (ROC) than the rest of the measurements (area-under-curve 0.83, where a cut-off value of 32.4% had 91.7% sensitivity and 77.8% specificity).Conclusions: One of the best non-invasive parameters in the detection of acute sub-clinical rejection appears to be the expression of CD4+/HLA-DR+ cells. Among ultrasound markers the best predictor of acute rejection is global radial systolic strain.</abstract>

Faculty of Medicine, Vilnius University, Vilnius, Lithuania

Faculty of Medicine, Vilnius University, M.K. Čiurlionio str. 21, LT-03101 Vilnius, Lithuania. Tel.: +37061473160

Clinic of Cardiovascular Diseases, Faculty of Medicine, Vilnius University, Vilnius, Lithuania

Clinic of Children’s Diseases, Faculty of Medicine, Vilnius University, Vilnius, Lithuania

The potential role of ultrasonic strain imaging and immunophenotyping in diagnosing acute rejection after heart transplantation

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Impact on porcine heart valve tissue treating with carbodiimide

The potential role of ultrasonic strain imaging and immunophenotyping in diagnosing acute rejection after heart transplantation

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