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Rare disease day in Slovakia, Europlan national conference

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Clinical Pharmacy in the Slovak Republic, dedicated to the associated professor Lívia Magulová, PhD.

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Conference abstracts, Rare disease day, conference 29.2.2012, First Slovak conference about rare diseases

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Détails du magazine
Format
Magazine
eISSN
2453-6725
Première publication
25 Nov 2011
Période de publication
2 fois par an
Langues
Anglais

Chercher

Volume 64 (2017): Edition 2 (November 2017)

Détails du magazine
Format
Magazine
eISSN
2453-6725
Première publication
25 Nov 2011
Période de publication
2 fois par an
Langues
Anglais

Chercher

7 Articles
Accès libre

Partial molecular volumes of cholesterol and phosphatidylcholine in mixed bilayers

Publié en ligne: 30 Nov 2017
Pages: 1 - 3

Résumé

Abstract

Dispersion of multilamellar liposomes of dimyristoylphosphatidylcholine (DMPC) and cholesterol (CHOL) were studied by vibrational densitometer for the CHOL mole fractions X = 0−0.54 in the temperature range 18−50 °C, both below and above the main phase transition. DMPC-CHOL bilayers served as a simple model for lipidic part of biological membrane. Volumetric parameters are essential not only to evaluate the data obtained by scattering and diffraction methods on model membranes but can provide valuable information about molecular packing in bilayers and the phase behaviour of lipid-CHOL mixtures. In this paper, preliminary results regarding the changes in the specific volume of lipid bilayer with increasing temperature and CHOL content are presented. Different values of apparent molecular volume of CHOL for different CHOL mole fraction pointed out the non-ideal mixing of DMPC and CHOL.

Mots clés

  • cholesterol
  • dimyristoylphosphatidylcholine
  • specific volume
  • densitometry
Accès libre

Effect of alkan-1-ols on the structure of dopc model membrane

Publié en ligne: 30 Nov 2017
Pages: 4 - 8

Résumé

Abstract

The effect of general anaesthetics alkan-1-ols (CnOH, where n = 10, 12, 14, 16 and 18 is the number of carbon atoms in the molecule) on the structure of dioleoylphosphatidylcholine (DOPC) model membrane was studied by small-angle neutron scattering (SANS) and small-angle neutron diffraction (SAND). Fluid bilayers were prepared at CnOH:DOPC = 0.3 molar ratio. The results of both the experiments show that bilayer thickness - a thickness parameter dg in the case of SANS and lamellar repeat distance D in the case of SAND - increases with increasing n. A coexistence of two lamellar phases with different D was detected by measuring the C18OH+DOPC oriented sample.

Mots clés

  • Model membrane - general anaesthetics - alkan-1-ols - small-angle neutron scattering - small-angle neutron diffraction - structure
Accès libre

Cation-containing lipid membranes – experiment and md simulations

Publié en ligne: 30 Nov 2017
Pages: 9 - 14

Résumé

Abstract

Using small angle neutron diffraction and molecular dynamics simulations we studied the interactions between calcium (Ca2+) or zinc (Zn2+) cations, and oriented gel phase dipalmitoyl-phosphatidylcholine (DPPC) bilayers. For both cations studied at ~1:7 divalent metal ion to lipid molar ratio (Me2+:DPPC), bilayer thickness increased. Simulation results helped reveal subtle differences in the effects of the two cations on gel phase membranes.

Mots clés

  • Lipid bilayer - metal ions - small angle neutron diffraction - MD simulations - simulation-to-experiment analysis
Accès libre

Regulation of the CaV3.2 calcium channels in health and disease Regulácia CaV3.2 vápnikových kanálov v zdraví a chorobe

Publié en ligne: 30 Nov 2017
Pages: 15 - 21

Résumé

Abstract

Family of T-type or low-voltage activated calcium channels consists of three members: CaV3.1, CaV3.2, and CaV3.3. CaV3.2 channel has almost identical biophysical properties as the CaV3.1 channel, but is distinguished by a specific tissue expression profile and a prominent role in several pathologies, including neuropathic pain, epilepsy, and dysregulation of cardiac rhythm. Further, it may be involved in phenotype of autism spectrum disorders, and amyotrophic lateral sclerosis. It represents a promising target for future pharmacotherapies.

Mots clés

  • T-type calcium channel - CaV3.2 - regulation - trafficking - glycosylation
Accès libre

Mechanical properties of lipid bilayers

Publié en ligne: 30 Nov 2017
Pages: 22 - 23

Résumé

Mots clés

  • Bending modulus - Tilt modulus - x-ray scattering
Accès libre

Effect of urea and tmao on lipid bilayers

Publié en ligne: 30 Nov 2017
Pages: 24 - 27

Résumé

Abstract

We study the effect of the osmolytes, Urea and trimethylamine-N-oxide (TMAO) on POPE (1-palmitoyl-2-oleoyl-sn-glycero-3- phosphoethanolamine) lipid membranes using SAXS/WAXS and DSC. Their antagonist effect is observed with TMAO stabilizing and Urea destabilizing the lipid bilayer, as seen by others in earlier researches.

Mots clés

  • lipid membranes - phase transitions - osmocytes - X-rays - DSC
Accès libre

Ceramides in the skin barrier

Publié en ligne: 30 Nov 2017
Pages: 28 - 35

Résumé

Abstract

The skin barrier, which is essential for human survival on dry land, is located in the uppermost skin layer, the stratum corneum. The stratum corneum consists of corneocytes surrounded by multilamellar lipid membranes that prevent excessive water loss from the body and entrance of undesired substances from the environment. To ensure this protective function, the composition and organization of the lipid membranes is highly specialized. The major skin barrier lipids are ceramides, fatty acids and cholesterol in an approximately equimolar ratio. With hundreds of molecular species of ceramide, skin barrier lipids are a highly complex mixture that complicate the investigation of its behaviour. In this minireview, the structures of the major skin barrier lipids, formation of the stratum corneum lipid membranes and their molecular organization are described.

Mots clés

  • Skin barrier - stratum corneum - ceramide - sphingolipid
7 Articles
Accès libre

Partial molecular volumes of cholesterol and phosphatidylcholine in mixed bilayers

Publié en ligne: 30 Nov 2017
Pages: 1 - 3

Résumé

Abstract

Dispersion of multilamellar liposomes of dimyristoylphosphatidylcholine (DMPC) and cholesterol (CHOL) were studied by vibrational densitometer for the CHOL mole fractions X = 0−0.54 in the temperature range 18−50 °C, both below and above the main phase transition. DMPC-CHOL bilayers served as a simple model for lipidic part of biological membrane. Volumetric parameters are essential not only to evaluate the data obtained by scattering and diffraction methods on model membranes but can provide valuable information about molecular packing in bilayers and the phase behaviour of lipid-CHOL mixtures. In this paper, preliminary results regarding the changes in the specific volume of lipid bilayer with increasing temperature and CHOL content are presented. Different values of apparent molecular volume of CHOL for different CHOL mole fraction pointed out the non-ideal mixing of DMPC and CHOL.

Mots clés

  • cholesterol
  • dimyristoylphosphatidylcholine
  • specific volume
  • densitometry
Accès libre

Effect of alkan-1-ols on the structure of dopc model membrane

Publié en ligne: 30 Nov 2017
Pages: 4 - 8

Résumé

Abstract

The effect of general anaesthetics alkan-1-ols (CnOH, where n = 10, 12, 14, 16 and 18 is the number of carbon atoms in the molecule) on the structure of dioleoylphosphatidylcholine (DOPC) model membrane was studied by small-angle neutron scattering (SANS) and small-angle neutron diffraction (SAND). Fluid bilayers were prepared at CnOH:DOPC = 0.3 molar ratio. The results of both the experiments show that bilayer thickness - a thickness parameter dg in the case of SANS and lamellar repeat distance D in the case of SAND - increases with increasing n. A coexistence of two lamellar phases with different D was detected by measuring the C18OH+DOPC oriented sample.

Mots clés

  • Model membrane - general anaesthetics - alkan-1-ols - small-angle neutron scattering - small-angle neutron diffraction - structure
Accès libre

Cation-containing lipid membranes – experiment and md simulations

Publié en ligne: 30 Nov 2017
Pages: 9 - 14

Résumé

Abstract

Using small angle neutron diffraction and molecular dynamics simulations we studied the interactions between calcium (Ca2+) or zinc (Zn2+) cations, and oriented gel phase dipalmitoyl-phosphatidylcholine (DPPC) bilayers. For both cations studied at ~1:7 divalent metal ion to lipid molar ratio (Me2+:DPPC), bilayer thickness increased. Simulation results helped reveal subtle differences in the effects of the two cations on gel phase membranes.

Mots clés

  • Lipid bilayer - metal ions - small angle neutron diffraction - MD simulations - simulation-to-experiment analysis
Accès libre

Regulation of the CaV3.2 calcium channels in health and disease Regulácia CaV3.2 vápnikových kanálov v zdraví a chorobe

Publié en ligne: 30 Nov 2017
Pages: 15 - 21

Résumé

Abstract

Family of T-type or low-voltage activated calcium channels consists of three members: CaV3.1, CaV3.2, and CaV3.3. CaV3.2 channel has almost identical biophysical properties as the CaV3.1 channel, but is distinguished by a specific tissue expression profile and a prominent role in several pathologies, including neuropathic pain, epilepsy, and dysregulation of cardiac rhythm. Further, it may be involved in phenotype of autism spectrum disorders, and amyotrophic lateral sclerosis. It represents a promising target for future pharmacotherapies.

Mots clés

  • T-type calcium channel - CaV3.2 - regulation - trafficking - glycosylation
Accès libre

Mechanical properties of lipid bilayers

Publié en ligne: 30 Nov 2017
Pages: 22 - 23

Résumé

Mots clés

  • Bending modulus - Tilt modulus - x-ray scattering
Accès libre

Effect of urea and tmao on lipid bilayers

Publié en ligne: 30 Nov 2017
Pages: 24 - 27

Résumé

Abstract

We study the effect of the osmolytes, Urea and trimethylamine-N-oxide (TMAO) on POPE (1-palmitoyl-2-oleoyl-sn-glycero-3- phosphoethanolamine) lipid membranes using SAXS/WAXS and DSC. Their antagonist effect is observed with TMAO stabilizing and Urea destabilizing the lipid bilayer, as seen by others in earlier researches.

Mots clés

  • lipid membranes - phase transitions - osmocytes - X-rays - DSC
Accès libre

Ceramides in the skin barrier

Publié en ligne: 30 Nov 2017
Pages: 28 - 35

Résumé

Abstract

The skin barrier, which is essential for human survival on dry land, is located in the uppermost skin layer, the stratum corneum. The stratum corneum consists of corneocytes surrounded by multilamellar lipid membranes that prevent excessive water loss from the body and entrance of undesired substances from the environment. To ensure this protective function, the composition and organization of the lipid membranes is highly specialized. The major skin barrier lipids are ceramides, fatty acids and cholesterol in an approximately equimolar ratio. With hundreds of molecular species of ceramide, skin barrier lipids are a highly complex mixture that complicate the investigation of its behaviour. In this minireview, the structures of the major skin barrier lipids, formation of the stratum corneum lipid membranes and their molecular organization are described.

Mots clés

  • Skin barrier - stratum corneum - ceramide - sphingolipid

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