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<p>The capability of the biosurfactant-producing strain <italic>Rhodococcus wratislawiensis</italic> BN38 to mineralize both aromatic and aliphatic xeno- biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by <italic>Rhodococcus</italic> free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l n-hexadecane (40 cycles). The alkanotrophic strain <italic>R. wratislawiensis</italic> BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous super-macroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water.
</p>
</abstract>

The capability of the biosurfactant-producing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno- biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by Rhodococcus free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l n-hexadecane (40 cycles). The alkanotrophic strain R. wratislawiensis BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous super-macroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water.

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain <italic>Rhodococcus wratislawiensis</italic> BN38

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain  BN38

The capability of the biosurfactant-producing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno- biotics was proved....

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Publié en ligne: 26 août 2016

Pages: 287 - 293

Reçu: 20 oct. 2014

Accepté: 11 févr. 2016

DOI: https://doi.org/10.5604/pjm-2016-287

Mots clés
sp., biodegradation, immobilization, n-hexadecane, phenol

© 2016 ALEXANDER E. HRISTOV et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Publié en ligne: 26 août 2016

Pages: 287 - 293

Reçu: 20 oct. 2014

Accepté: 11 févr. 2016

DOI: https://doi.org/10.5604/pjm-2016-287

Mots cléssp., biodegradation, immobilization, n-hexadecane, phenol

© 2016 ALEXANDER E. HRISTOV et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
sp., biodegradation, immobilization, n-hexadecane, phenol