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<p>We report the genetic similarity changes between a mutant mushroom (<italic>Pleurotus florida</italic>, designated as PfCM4) having increased cellulolytic activity developed through radiation mutagenesis and its wild type by amplified fragment length polymorphism (AFLP). On average, 23 AFLP fragments were amplified per primer combination, and a total of 286 polymorphic fragments (78.57% polymorphism) with maximal fragment length of 1365 base pairs (bp) were obtained. The genetic similarity between wild type and PfCM4 was found to be 22.30%. In addition, mycelial and secreted protein profiling by 2D-PAGE showed at least three and five different protein spots in the range of 25 kD to 100 kD, respectively, in PfCM4. It seems that the variation in genetic similarity and different expression of both mycelial and secreted proteins in PfCM4 in comparison to the wild type could likely be correlated with its increased cellulolytic activity effected by the irradiation.</p>
</abstract>

We report the genetic similarity changes between a mutant mushroom (Pleurotus florida, designated as PfCM4) having increased cellulolytic activity developed through radiation mutagenesis and its wild type by amplified fragment length polymorphism (AFLP). On average, 23 AFLP fragments were amplified per primer combination, and a total of 286 polymorphic fragments (78.57% polymorphism) with maximal fragment length of 1365 base pairs (bp) were obtained. The genetic similarity between wild type and PfCM4 was found to be 22.30%. In addition, mycelial and secreted protein profiling by 2D-PAGE showed at least three and five different protein spots in the range of 25 kD to 100 kD, respectively, in PfCM4. It seems that the variation in genetic similarity and different expression of both mycelial and secreted proteins in PfCM4 in comparison to the wild type could likely be correlated with its increased cellulolytic activity effected by the irradiation.

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom <italic>Pleurotus florida</italic>

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom 

We report the genetic similarity changes between a mutant mushroom (Pleurotus florida, designated as PfCM4) having increased cellulolytic activity developed...

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

Publié en ligne: 26 août 2016

Pages: 271 - 277

Reçu: 23 sept. 2013

Accepté: 11 févr. 2016

DOI: https://doi.org/10.5604/pjm-2016-271

Mots clés
2D-PAGE, AFLP, genetic variability, mushroom

© 2016 CHANDRAN SATHESH-PRABU et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Genetic Variability and Proteome Profiling of a Radiation Induced Cellulase Mutant Mushroom Pleurotus florida

Publié en ligne: 26 août 2016

Pages: 271 - 277

Reçu: 23 sept. 2013

Accepté: 11 févr. 2016

DOI: https://doi.org/10.5604/pjm-2016-271

Mots clés2D-PAGE, AFLP, genetic variability, mushroom

© 2016 CHANDRAN SATHESH-PRABU et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
2D-PAGE, AFLP, genetic variability, mushroom