<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil microbial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus <italic>Bacillus</italic> sp. and were identified by cultivation and 16S rRNA methods. The commercially available procedures for bacterial DNA isolation from acidified soil failed, therefore a new, specific DNA isolation method was established. Ureolytic activity, detected in soil extracts as well as in isolated <italic>Bacillus</italic> sp. strains may be considered as a tool for the bioremediation of acidified soils with high sulfate content.</p>
</abstract>

Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic, oxidoreductive, and lipolytic activity were detected. The presented results indicate that the enzymatic activity of soil microbial communities varied considerably over time. Isolated 26 (80%) bacterial strains belonged to genus Bacillus sp. and were identified by cultivation and 16S rRNA methods. The commercially available procedures for bacterial DNA isolation from acidified soil failed, therefore a new, specific DNA isolation method was established. Ureolytic activity, detected in soil extracts as well as in isolated Bacillus sp. strains may be considered as a tool for the bioremediation of acidified soils with high sulfate content.

Characterization of Microbial Communities in Acidified, Sulfur Containing Soils

Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

{"article-title":"Characterization of Microbial Communities in Acidified, Sulfur Containing Soils"}

Over a period of three years, microbial communities in acidified soil with high sulfur content were analyzed. In soil water extracts ureolytic, proteolytic,...

Characterization of Microbial Communities in Acidified, Sulfur Containing Soils

Publié en ligne: 04 déc. 2017

Pages: 509 - 517

Reçu: 03 mars 2017

Accepté: 13 juin 2017

DOI: https://doi.org/10.5604/01.3001.0010.7043

Mots clés
acidified soil microflora, DNA isolation specific method, soil bacteria, soil biochemical activity

© 2017 GRZEGORZ CZERWONKA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Characterization of Microbial Communities in Acidified, Sulfur Containing Soils

Publié en ligne: 04 déc. 2017

Pages: 509 - 517

Reçu: 03 mars 2017

Accepté: 13 juin 2017

DOI: https://doi.org/10.5604/01.3001.0010.7043

Mots clésacidified soil microflora, DNA isolation specific method, soil bacteria, soil biochemical activity

© 2017 GRZEGORZ CZERWONKA et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Mots clés
acidified soil microflora, DNA isolation specific method, soil bacteria, soil biochemical activity