<abstract xmlns="http://www.w3.org/1999/xhtml"> Background: Villin is a protein that severs, binds, and bundles actin filaments, necessary for maintenance of the structural integrity of the microvilli in the absorptive epithelia, which include enterocytes, kidney proximal tubule cells, and bile canaliculi. Biliary atresia (BA) is a progressive, sclerosing, inflammatory process that results in complete obliteration of the extrahepatic bile ducts. Liver histopathology in BA might be similar to idiopathic neonatal hepatitis (INH).Objective: We compared the hepatic expression of villin in children with BA and INH.Methods: This study was conducted on stored liver specimens of 28 children with BA (age 2.78±1.33 months) and 10 children with INH (age 2.40±1.26 months). Villin was investigated on liver tissue by immunohistochemistry. Villin mRNA analyzes were undertaken on liver homogenates using real-time PCR technique, with primers from coding regions of the human villin gene. Amounts of villin PCR product (villin mRNA relative to the concentrations of GAPDH product) were expressed as median (minimum, maximum).Results: Villin mRNA expression in patients with BA and INH were 15.98 (0.02, 11880.47) and 0.09 (0, 111.95), respectively. Villin mRNA expression in BA patients was significantly higher than in patients with INH (p = 0.01). Villin staining and mRNA expression were undetectable in two INH.Conclusions: The higher villin expression in BA might have been due to pronounced bile duct proliferation in comparison with INH. Hepatic villin expression might be helpful in discrimination BA from INH and hence BA patients can be timely diagnosed.</abstract>

Background: Villin is a protein that severs, binds, and bundles actin filaments, necessary for maintenance of the structural integrity of the microvilli in the absorptive epithelia, which include enterocytes, kidney proximal tubule cells, and bile canaliculi. Biliary atresia (BA) is a progressive, sclerosing, inflammatory process that results in complete obliteration of the extrahepatic bile ducts. Liver histopathology in BA might be similar to idiopathic neonatal hepatitis (INH).Objective: We compared the hepatic expression of villin in children with BA and INH.Methods: This study was conducted on stored liver specimens of 28 children with BA (age 2.78±1.33 months) and 10 children with INH (age 2.40±1.26 months). Villin was investigated on liver tissue by immunohistochemistry. Villin mRNA analyzes were undertaken on liver homogenates using real-time PCR technique, with primers from coding regions of the human villin gene. Amounts of villin PCR product (villin mRNA relative to the concentrations of GAPDH product) were expressed as median (minimum, maximum).Results: Villin mRNA expression in patients with BA and INH were 15.98 (0.02, 11880.47) and 0.09 (0, 111.95), respectively. Villin mRNA expression in BA patients was significantly higher than in patients with INH (p = 0.01). Villin staining and mRNA expression were undetectable in two INH.Conclusions: The higher villin expression in BA might have been due to pronounced bile duct proliferation in comparison with INH. Hepatic villin expression might be helpful in discrimination BA from INH and hence BA patients can be timely diagnosed.

Hepatic villin expression in biliary atresia

Assoc. Prof. MD. Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Department of Pathology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,

Asian Biomedicine

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

{"article-title":"Hepatic villin expression in biliary atresia"}

Background: Villin is a protein that severs, binds, and bundles actin filaments, necessary for maintenance of the structural integrity of the microvilli in the...

Hepatic villin expression in biliary atresia

Article Category: Original article

Publié en ligne: 04 févr. 2017

Pages: 731 - 736

DOI: https://doi.org/10.5372/1905-7415.0605.114

Mots clés
Actin, biliary atresia, cholestatic liver disease, idiopathic neonatal hepatitis, villin

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Hepatic villin expression in biliary atresia

Article Category: Original article

Publié en ligne: 04 févr. 2017

Pages: 731 - 736

DOI: https://doi.org/10.5372/1905-7415.0605.114

Mots clésActin, biliary atresia, cholestatic liver disease, idiopathic neonatal hepatitis, villin

© 2017

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Mots clés
Actin, biliary atresia, cholestatic liver disease, idiopathic neonatal hepatitis, villin