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Inflammation and Hypoxia Negatively Impact the Survival and Immunosuppressive Properties of Mesenchymal Stromal Cells In Vitro

Carmen Alexandra Neculachi
Carmen Alexandra Neculachi
, 
Livia Ioana Leti
Livia Ioana Leti
, 
Alexandrina Burlacu
Alexandrina Burlacu
 et 
Mihai Bogdan Preda
Mihai Bogdan Preda
   | 05 mai 2022
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Article Category: Original Article
Publié en ligne: 05 mai 2022
Pages: 547 - 554
DOI: https://doi.org/10.47803/rjc.2021.31.3.547
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© 2021 Carmen Alexandra Neculachi et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Figure 1

TNFα and IFNγ differentially regulate immunomodulatory factors in murine MSC. A. Phase contrast microscopy images illustrating the morphology of MSC after in vitro stimulation with TNFα and IFNγ for 24 hours. B–D. Gene expression levels of IDO1, NOS2 and COX2 in MSC stimulated with TNFα and IFNγ for 24 hours.
TNFα and IFNγ differentially regulate immunomodulatory factors in murine MSC. A. Phase contrast microscopy images illustrating the morphology of MSC after in vitro stimulation with TNFα and IFNγ for 24 hours. B–D. Gene expression levels of IDO1, NOS2 and COX2 in MSC stimulated with TNFα and IFNγ for 24 hours.

Figure 2

Prolonged inflammation affects the morphology and viability of MSC. A) xCELLigence analysis on MSC viability, correlated with the cellular index variation B) Phase contrast microscopy images showing the cellular morphology of MSC after stimulation with TNFα and IFNγ after 48 hours of cytokine treatment; C) xCELLigence analysis of MSC viability after stimulated with three different doses of TNFα; D) Phase contrast microscopy images showing the MSC viability after 72-hour stimulation with TNFα at different concentrations.
Prolonged inflammation affects the morphology and viability of MSC. A) xCELLigence analysis on MSC viability, correlated with the cellular index variation B) Phase contrast microscopy images showing the cellular morphology of MSC after stimulation with TNFα and IFNγ after 48 hours of cytokine treatment; C) xCELLigence analysis of MSC viability after stimulated with three different doses of TNFα; D) Phase contrast microscopy images showing the MSC viability after 72-hour stimulation with TNFα at different concentrations.

Figure 3

L-NAME effect on MSC mortality and NO production. A) Phase contrast microscopy images showing the cellular morphology of MSC after stimulation with TNFα and IFNγ, alone or in combination, in the presence or absence of NOS2 inhibitor L-NAME. B) Quantitative determination of nitrite produced by MSC after stimulation with TNFα and IFNγ.
L-NAME effect on MSC mortality and NO production. A) Phase contrast microscopy images showing the cellular morphology of MSC after stimulation with TNFα and IFNγ, alone or in combination, in the presence or absence of NOS2 inhibitor L-NAME. B) Quantitative determination of nitrite produced by MSC after stimulation with TNFα and IFNγ.

Figure 4

Low oxygen levels and cytokines affect MSC viability. A) Phase contrast microscopy images showing the cellular morphology of MSC after stimulation with TNFα and IFNγ in 21% (Normoxia) vs 2% (Hypoxia) O2. B) Quantitative determination of the L-NAME MSC produced when stimulated with TNFα and IFNγ in 21% vs 2% O2.
Low oxygen levels and cytokines affect MSC viability. A) Phase contrast microscopy images showing the cellular morphology of MSC after stimulation with TNFα and IFNγ in 21% (Normoxia) vs 2% (Hypoxia) O2. B) Quantitative determination of the L-NAME MSC produced when stimulated with TNFα and IFNγ in 21% vs 2% O2.
eISSN:
2734-6382
Langue:
Anglais
Périodicité:
4 fois par an
Sujets de la revue:
Medicine, Clinical Medicine, Internal Medicine, Cardiology, Pediatrics and Juvenile Medicine, Paediatric Cardiology, Surgery, Cardiac Surgery
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