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The effect of the release of exogenous nitric oxide on the responses of the pregnant human myometrium to oxytocin*

Beata Modzelewska
Beata Modzelewska
, 
Tomasz Kleszczewski
Tomasz Kleszczewski
 et 
Anna Kostrzewska
Anna Kostrzewska
   | 14 janv. 2019
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Article Category: Original Article/Praca Oryginalna
Publié en ligne: 14 janv. 2019
Pages: 301 - 307
Reçu: 29 août 2018
Accepté: 19 sept. 2018
DOI: https://doi.org/10.34763/devperiodmed.20182204.301307
© 2018 Beata Modzelewska, Tomasz Kleszczewski, Anna Kostrzewska, published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.

Fig. 1

A representative recording of spontaneous contractile activity of the myometrial strips and the effect of cumulatively administered OXT (10-14– 10-6 mol/L): (A) without any blocker and (B) after preincubation with L-NNA (3·10-4 mol/L) and (C) incubation with DETA/NO (10-4 mol/L).
A representative recording of spontaneous contractile activity of the myometrial strips and the effect of cumulatively administered OXT (10-14– 10-6 mol/L): (A) without any blocker and (B) after preincubation with L-NNA (3·10-4 mol/L) and (C) incubation with DETA/NO (10-4 mol/L).

Fig. 2

Effects of DETA/NO (20 min or 20 hours) and L-NNA (20 min) incubation on the pregnant myometrium, as measured by the area under the curve. Spontaneous contractile activity after 20 min observation was treated as control. *- p<0.05.
Effects of DETA/NO (20 min or 20 hours) and L-NNA (20 min) incubation on the pregnant myometrium, as measured by the area under the curve. Spontaneous contractile activity after 20 min observation was treated as control. *- p<0.05.

Fig. 3

Effects of OXT (range 10-14–10-6 mol/L) on the pregnant myometrium, as measured by the area under the curve. (□) – OXT alone, (▪) – after preincubation with 3·10-4 mol/L L-NNA, (▴) – after incubation with 10-4 mol/L DETA/NO, (•) – after preincubation with 3·10-4 mol/L L-NNA followed by incubation of 10-4 mol/L DETA/NO. Each point represents the mean ± SEM of N =8 individual myometrial strips from different patients. Spontaneous contractions of the myometrial strips before OXT were treated as a control.
Effects of OXT (range 10-14–10-6 mol/L) on the pregnant myometrium, as measured by the area under the curve. (□) – OXT alone, (▪) – after preincubation with 3·10-4 mol/L L-NNA, (▴) – after incubation with 10-4 mol/L DETA/NO, (•) – after preincubation with 3·10-4 mol/L L-NNA followed by incubation of 10-4 mol/L DETA/NO. Each point represents the mean ± SEM of N =8 individual myometrial strips from different patients. Spontaneous contractions of the myometrial strips before OXT were treated as a control.

Log EC50 and Emax for OXT (range 10-14 - 10-6 mol/L) on spontaneous contractions of the pregnant myometrium. The values are mean ± SEM of N individual myometrial strips from different patients. N=6 for all comparisons which were done in reference to control. *p<0.05 versus the Group 1; †p<0.05 versus the Group 3.

Groups OXT
logEC 50 Emax
Group 1 (no blocker or NO donor - Control) -7.61±0.18 367.30±51.53
Group 2 (preincubation with L-NNA) -7.64±0.18 482.70±54.27
Group 3 (preincubation with DETA/NO) -6.68±1.05 174.10±73.08*
Group 4 (preincubation with L-NNA + DETA/NO) -6.97±0.39 426.60±46.88†
eISSN:
2719-535X
Langue:
Anglais
Périodicité:
Volume Open
Sujets de la revue:
Medicine, Clinical Medicine, Pediatrics and Juvenile Medicine, Paediatric Haematology and Oncology, Public Health
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