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Isolation and Electrochemical Analysis of a Facultative Anaerobic Electrogenic Strain Klebsiella sp. SQ-1

Lei Zhou

Lei Zhou

Henan Provincial Engineering Research Center for Development and Application of Characteristic Microorganism Resources, College of Biology and Food, Shangqiu Normal UniversityShangqiu, PR China
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Zhou, Lei
, 
Tuoxian Tang

Tuoxian Tang

Department of Biological Sciences, Virginia TechBlacksburg, USA
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Tang, Tuoxian
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Dandan Deng

Dandan Deng

Henan Provincial Engineering Research Center for Development and Application of Characteristic Microorganism Resources, College of Biology and Food, Shangqiu Normal UniversityShangqiu, PR China
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Deng, Dandan
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Yayue Wang

Yayue Wang

Henan Provincial Engineering Research Center for Development and Application of Characteristic Microorganism Resources, College of Biology and Food, Shangqiu Normal UniversityShangqiu, PR China
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Wang, Yayue
 et 
Dongli Pei

Dongli Pei

Henan Provincial Engineering Research Center for Development and Application of Characteristic Microorganism Resources, College of Biology and Food, Shangqiu Normal UniversityShangqiu, PR China
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Pei, Dongli
  
27 avr. 2024
Isolation and Electrochemical Analysis of a Facultative Anaerobic Electrogenic Strain Klebsiella sp. SQ-1's Cover Image
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Catégorie d'article: Original Paper
Publié en ligne: 27 avr. 2024
Pages: 143 - 153
Reçu: 07 déc. 2023
Accepté: 26 févr. 2024
DOI: https://doi.org/10.33073/pjm-2024-013
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© 2024 Lei Zhou et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Fig. 1.

Enrichment, isolation, and identification of the strain SQ-1 A) The output voltage performance of mixed microflora in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. B) The output voltage (circle) and power density (square) curves for mixed microflora. C) Phylogenetic analysis of the strain SQ-1 based on 16S rDNA gene sequences. D) The appearance of the strain SQ-1 and Geobacter sulfurreducens PCA in liquid growth medium after 3 days of growth.
Enrichment, isolation, and identification of the strain SQ-1 A) The output voltage performance of mixed microflora in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. B) The output voltage (circle) and power density (square) curves for mixed microflora. C) Phylogenetic analysis of the strain SQ-1 based on 16S rDNA gene sequences. D) The appearance of the strain SQ-1 and Geobacter sulfurreducens PCA in liquid growth medium after 3 days of growth.

Fig. 2.

Output voltage and power density of Klebsiella sp. SQ-1 in MFC A) The output voltage performance of the strain SQ-1 in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. B) The output voltage performance of a model electricigen Geobacter sulfurreducens PCA in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. C) The output voltage (circle) and power density (square) curve for the strain SQ-1. D) The output voltage (circle) and power density (square) curves for G. sulfurreducens PCA. E) Comparison of the maximum output voltage of mixed microflora, G. sulfurreducens PCA, and Klebsiella sp. SQ-1 in MFC. F) Comparison of maximum output power density of mixed microflora, G. sulfurreducens PCA, and Klebsiella sp. SQ-1 in MFC.
Output voltage and power density of Klebsiella sp. SQ-1 in MFC A) The output voltage performance of the strain SQ-1 in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. B) The output voltage performance of a model electricigen Geobacter sulfurreducens PCA in a dual-chamber MFC using sodium acetate as a carbon source. Arrows show the supply of sodium acetate. C) The output voltage (circle) and power density (square) curve for the strain SQ-1. D) The output voltage (circle) and power density (square) curves for G. sulfurreducens PCA. E) Comparison of the maximum output voltage of mixed microflora, G. sulfurreducens PCA, and Klebsiella sp. SQ-1 in MFC. F) Comparison of maximum output power density of mixed microflora, G. sulfurreducens PCA, and Klebsiella sp. SQ-1 in MFC.

Fig. 3.

Electrochemical performance of Klebsiella sp. SQ-1 in a three-electrode system A) Chronoamperometrlc curves for the strain SQ-1 In a three-electrode system using sodium acetate as electron acceptors. Inset, cyclic voltammograms of the enriched strain SQ-1 biofilm on electrodes in dltferent stages; a) before blofllm formation; b) after blofllm formation with a current density below 2 μA cm–2. B(1) The morphology of the strain SQ-1 blofllms under the scanning electron microscope (5,000x). Blofllms were attached to the graphite surface. B(2) The appearance of bacterial culture after 5 days of Incubation when the growth medium Is supplemented with lron(III) oxide. A magnet Is placed outside the tube. The arrow shows that the magnet attracts the black suspension. B(3) The Initial appearance of bacterial culture. C) Voltammograms of the strain SQ-1 blofllm. Scan rates: 1, 2, 5, 10, 20, 50 mV s–1. Inset, the linear dependence of the cathodic peak current density at -0.37 V versus scan rates or square roots of scan rates. D) The Nyquist plot for blofllms attached to working electrodes.
Electrochemical performance of Klebsiella sp. SQ-1 in a three-electrode system A) Chronoamperometrlc curves for the strain SQ-1 In a three-electrode system using sodium acetate as electron acceptors. Inset, cyclic voltammograms of the enriched strain SQ-1 biofilm on electrodes in dltferent stages; a) before blofllm formation; b) after blofllm formation with a current density below 2 μA cm–2. B(1) The morphology of the strain SQ-1 blofllms under the scanning electron microscope (5,000x). Blofllms were attached to the graphite surface. B(2) The appearance of bacterial culture after 5 days of Incubation when the growth medium Is supplemented with lron(III) oxide. A magnet Is placed outside the tube. The arrow shows that the magnet attracts the black suspension. B(3) The Initial appearance of bacterial culture. C) Voltammograms of the strain SQ-1 blofllm. Scan rates: 1, 2, 5, 10, 20, 50 mV s–1. Inset, the linear dependence of the cathodic peak current density at -0.37 V versus scan rates or square roots of scan rates. D) The Nyquist plot for blofllms attached to working electrodes.

Fig. 4.

Ferric reduction of Klebsiella sp. SQ-1 A) The growth curve of strain SQ-1 measured under aerobic and anaerobic conditions. B) The growth curve of strain SQ-1 when the growth medium was supplemented with different amounts of ferric citrate. C) Measurement of reduced Fe2+. D) Analysis of Fe3+ reduction ratio. The concentration of Fe2+ was measured after 30 hours of incubation.
Ferric reduction of Klebsiella sp. SQ-1 A) The growth curve of strain SQ-1 measured under aerobic and anaerobic conditions. B) The growth curve of strain SQ-1 when the growth medium was supplemented with different amounts of ferric citrate. C) Measurement of reduced Fe2+. D) Analysis of Fe3+ reduction ratio. The concentration of Fe2+ was measured after 30 hours of incubation.

Electrical generation performance of reported electricigens_

Strains Substrates Voltage (V) Power density (mW m–A) Current density (μA cm–2) References
Klebsiella sp. SQ-1 sodium acetate 0.59 560 625 this work
Geobacter sulfurreducens PCA sodium acetate 0.47 460 605 (Deng et al. 2015)
Pseudomonas aeruginosa glucose / / 52.1 (Yong et al. 2017)
Klebsiella pneumonia glucose 0.621 40.26 / (Guo et al. 2020)
Shewanella oneidensis lactate / 41 / (Watson and Logan 2010)
Citrobacter freundii citrate / 204.5 / (Huang et al. 2015)
Escherichia coli glucose / / 34 (Qiao et al. 2009)
Rhodoferax ferrireducens glucose / / 14.6 (Liu et al. 2007)
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Sciences de la vie, Microbiologie et virologie
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