Cloning, Heterologous Expression, and Characterization of a Neutral Uricase from Arthrobacter sp. CSAJ-16 in Cangshan Mountain

Abdel-Fattah YR, Saeed HM, Gohar YM, El-Baz MA. Improved production of Pseudomonas aeruginosa uricase by optimization of process parameters through statistical experimental designs. Process Biochem. 2005 Apr;40(5):1707–1714. https://doi.org/10.1016/j.procbio.2004.06.048 Abdel-FattahYR SaeedHM GoharYM El-BazMA Improved production of Pseudomonas aeruginosa uricase by optimization of process parameters through statistical experimental designs Process Biochem 2005 Apr 40 5 1707 1714 https://doi.org/10.1016/j.procbio.2004.06.048 Search in Google Scholar

Bodofsky S, Merriman TR, Thomas TJ, Schlesinger N. Advances in our understanding of gout as an auto-inflammatory disease. Semin Arthritis Rheum. 2020 Oct;50(5):1089–1100. https://doi.org/10.1016/j.semarthrit.2020.06.015 BodofskyS MerrimanTR ThomasTJ SchlesingerN Advances in our understanding of gout as an auto-inflammatory disease Semin Arthritis Rheum 2020 Oct 50 5 1089 1100 https://doi.org/10.1016/j.semarthrit.2020.06.015 Search in Google Scholar

Bongaerts GP, Uitzetter J, Brouns R, Vogels GD. Uricase of Bacillus fastidiosus. Properties and regulation of synthesis. Biochim Biophys Acta. 1978 Dec;527(2):348–358. https://doi.org/10.1016/0005-2744(78)90349-2 BongaertsGP UitzetterJ BrounsR VogelsGD Uricase of Bacillus fastidiosus. Properties and regulation of synthesis Biochim Biophys Acta 1978 Dec 527 2 348 358 https://doi.org/10.1016/0005-2744(78)90349-2 Search in Google Scholar

Chiu YC, Hsu TS, Huang CY, Hsu CH. Molecular elucidation of a urate oxidase from Deinococcus radiodurans for hyperuricemia and gout therapy. Int J Mol Sci. 2021a May;22(11):5611. https://doi.org/10.3390/ijms22115611 ChiuYC HsuTS HuangCY HsuCH Molecular elucidation of a urate oxidase from Deinococcus radiodurans for hyperuricemia and gout therapy Int J Mol Sci 2021a May 22 11 5611 https://doi.org/10.3390/ijms22115611 Search in Google Scholar

Chiu YC, Hsu TS, Huang CY, Hsu CH. Structural and biochemical insights into a hyperthermostable urate oxidase from Thermobispora bispora for hyperuricemia and gout therapy. Int J Biol Macromol. 2021b Oct;188:914–923. https://doi.org/10.1016/j.ijbiomac.2021.08.081 ChiuYC HsuTS HuangCY HsuCH Structural and biochemical insights into a hyperthermostable urate oxidase from Thermobispora bispora for hyperuricemia and gout therapy Int J Biol Macromol 2021b Oct 188 914 923 https://doi.org/10.1016/j.ijbiomac.2021.08.081 Search in Google Scholar

Felten R, Duret PM, Gottenberg JE, Spielmann L, Messer L. At the crossroads of gout and psoriatic arthritis: “psout”. Clin Rheumatol. 2020 May;39(5):1405–1413. https://doi.org/10.1007/s10067-020-04981-0 FeltenR DuretPM GottenbergJE SpielmannL MesserL At the crossroads of gout and psoriatic arthritis: “psout” Clin Rheumatol 2020 May 39 5 1405 1413 https://doi.org/10.1007/s10067-020-04981-0 Search in Google Scholar

Grassi D, Ferri L, Desideri G, Di Giosia P, Cheli P, Del Pinto R, Properzi G, Ferri C. Chronic hyperuricemia, uric acid deposit and cardiovascular risk. Curr Pharm Des. 2013;19(13):2432–2438. https://doi.org/10.2174/1381612811319130011 GrassiD FerriL DesideriG Di GiosiaP CheliP Del PintoR ProperziG FerriC Chronic hyperuricemia, uric acid deposit and cardiovascular risk Curr Pharm Des 2013 19 13 2432 2438 https://doi.org/10.2174/1381612811319130011 Search in Google Scholar

Hansildaar R, Vedder D, Baniaamam M, Tausche AK, Gerritsen M, Nurmohamed MT. Cardiovascular risk in inflammatory arthritis: rheumatoid arthritis and gout. Lancet Rheumatol. 2021 Jan;3(1): e58–e70. https://doi.org/10.1016/S2665-9913(20)30221-6 HansildaarR VedderD BaniaamamM TauscheAK GerritsenM NurmohamedMT Cardiovascular risk in inflammatory arthritis: rheumatoid arthritis and gout Lancet Rheumatol 2021 Jan 3 1 e58 e70 https://doi.org/10.1016/S2665-9913(20)30221-6 Search in Google Scholar

Hu DE, Moore AM, Thomsen LL, Brindle KM. Uric acid promotes tumor immune rejection. Cancer Res. 2004 Aug;64(15):5059–5062. https://doi.org/10.1158/0008-5472.CAN-04-1586 HuDE MooreAM ThomsenLL BrindleKM Uric acid promotes tumor immune rejection Cancer Res 2004 Aug 64 15 5059 5062 https://doi.org/10.1158/0008-5472.CAN-04-1586 Search in Google Scholar

Khaleghi R, Asad S. Heterologous expression of recombinant urate oxidase using the intein-mediated protein purification in Pichia pastoris. 3 Biotech. 2021 Mar;11(3):120. https://doi.org/10.1007/s13205-021-02670-6 KhaleghiR AsadS Heterologous expression of recombinant urate oxidase using the intein-mediated protein purification in Pichia pastoris 3 Biotech 2021 Mar 11 3 120 https://doi.org/10.1007/s13205-021-02670-6 Search in Google Scholar

Koyama Y, Ichikawa T, Nakano E. Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding the Candida utilis urate oxidase (uricase). J Biochem. 1996 Nov;120(5):969–973. https://doi.org/10.1093/oxfordjournals.jbchem.a021514 KoyamaY IchikawaT NakanoE Cloning, sequence analysis, and expression in Escherichia coli of the gene encoding the Candida utilis urate oxidase (uricase) J Biochem 1996 Nov 120 5 969 973 https://doi.org/10.1093/oxfordjournals.jbchem.a021514 Search in Google Scholar

Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016 Jul;33(7):1870–1874. https://doi.org/10.1093/molbev/msw054 KumarS StecherG TamuraK MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets Mol Biol Evol 2016 Jul 33 7 1870 1874 https://doi.org/10.1093/molbev/msw054 Search in Google Scholar

Li J, Chen Z, Hou L, Fan H, Weng S, Xu C, Ren J, Li B, Chen W. High-level expression, purification, and characterization of non-tagged Aspergillus flavus urate oxidase in Escherichia coli. Protein Expr Purif. 2006 Sep;49(1):55–59. https://doi.org/10.1016/j.pep.2006.02.003 LiJ ChenZ HouL FanH WengS XuC RenJ LiB ChenW High-level expression, purification, and characterization of non-tagged Aspergillus flavus urate oxidase in Escherichia coli Protein Expr Purif 2006 Sep 49 1 55 59 https://doi.org/10.1016/j.pep.2006.02.003 Search in Google Scholar

Liu SY, Shibu MA, Jhan HJ, Lo CT, Peng KC. Purification and characterization of novel glucanases from Trichoderma harzianum ETS 323. J Agric Food Chem. 2010 Oct;58(19):10309–10314. https://doi.org/10.1021/jf1029338 LiuSY ShibuMA JhanHJ LoCT PengKC Purification and characterization of novel glucanases from Trichoderma harzianum ETS 323 J Agric Food Chem 2010 Oct 58 19 10309 10314 https://doi.org/10.1021/jf1029338 Search in Google Scholar

McCormick N, Rai SK, Lu N, Yokose C, Curhan GC, Choi HK. Estimation of primary prevention of gout in men through modification of obesity and other key lifestyle factors. JAMA Netw Open. 2020 Nov;3(11):e2027421. https://doi.org/10.1001/jamanetworkopen.2020.27421 McCormickN RaiSK LuN YokoseC CurhanGC ChoiHK Estimation of primary prevention of gout in men through modification of obesity and other key lifestyle factors JAMA Netw Open 2020 Nov 3 11 e2027421 https://doi.org/10.1001/jamanetworkopen.2020.27421 Search in Google Scholar

McLeod MP, Warren RL, Hsiao WW, Araki N, Myhre M, Fernandes C, Miyazawa D, Wong W, Lillquist AL, Wang D, et al. The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse. Proc Natl Acad Sci USA. 2006 Oct; 103(42): 15582–15587. https://doi.org/10.1073/pnas.0607048103 McLeodMP WarrenRL HsiaoWW ArakiN MyhreM FernandesC MiyazawaD WongW LillquistAL WangD The complete genome of Rhodococcus sp. RHA1 provides insights into a catabolic powerhouse Proc Natl Acad Sci USA 2006 Oct 103 42 15582 15587 https://doi.org/10.1073/pnas.0607048103 Search in Google Scholar

Reinders MK, Jansen TL. New advances in the treatment of gout: review of pegloticase. Ther Clin Risk Manag. 2010 Oct;6:543–550. https://doi.org/10.2147/TCRM.S6043 ReindersMK JansenTL New advances in the treatment of gout: review of pegloticase Ther Clin Risk Manag 2010 Oct 6 543 550 https://doi.org/10.2147/TCRM.S6043 Search in Google Scholar

Schumacher HR Jr, Chen LX. Newer therapeutic approaches: gout. Rheum Dis Clin North Am. 2006 Feb;32(1):235–244. https://doi.org/10.1016/j.rdc.2005.10.003 SchumacherHRJr ChenLX Newer therapeutic approaches: gout Rheum Dis Clin North Am 2006 Feb 32 1 235 244 https://doi.org/10.1016/j.rdc.2005.10.003 Search in Google Scholar

Sherman MR, Saifer MG, Perez-Ruiz F. PEG-uricase in the management of treatment-resistant gout and hyperuricemia. Adv Drug Deliv Rev. 2008 Jan;60(1):59–68. https://doi.org/10.1016/j.addr.2007.06.011 ShermanMR SaiferMG Perez-RuizF PEG-uricase in the management of treatment-resistant gout and hyperuricemia Adv Drug Deliv Rev 2008 Jan 60 1 59 68 https://doi.org/10.1016/j.addr.2007.06.011 Search in Google Scholar

Singh JA, Gaffo A. Gout epidemiology and comorbidities. Semin Arthritis Rheum. 2020 Jun;50(3S):S11–S16. https://doi.org/10.1016/j.semarthrit.2020.04.008 SinghJA GaffoA Gout epidemiology and comorbidities Semin Arthritis Rheum 2020 Jun 50 3S S11 S16 https://doi.org/10.1016/j.semarthrit.2020.04.008 Search in Google Scholar

Suzuki K, Sakasegawa S, Misaki H, Sugiyama M. Molecular cloning and expression of uricase gene from Arthrobacter globiformis in Escherichia coli and characterization of the gene product. J Biosci Bioeng. 2004;98(3):153–158. https://doi.org/10.1016/S1389-1723(04)00259-2 SuzukiK SakasegawaS MisakiH SugiyamaM Molecular cloning and expression of uricase gene from Arthrobacter globiformis in Escherichia coli and characterization of the gene product J Biosci Bioeng 2004 98 3 153 158 https://doi.org/10.1016/S1389-1723(04)00259-2 Search in Google Scholar

Tang L, Liu J, Liu X. [Construction and secetory expression of gene engineering bacteria of uric acid enzyme] (in Chinese). J Chongqing Med Univ. 2018;43(01):127–130. https://doi.org/10.13406/j.cnki.cyxb.000939 TangL LiuJ LiuX [Construction and secetory expression of gene engineering bacteria of uric acid enzyme] (in Chinese) J Chongqing Med Univ 2018 43 01 127 130 https://doi.org/10.13406/j.cnki.cyxb.000939 Search in Google Scholar

Vogels GD, Van der Drift C. Degradation of purines and pyrimi-dines by microorganisms. Bacteriol Rev. 1976 Jun;40(2):403–468. https://doi.org/10.1128/br.40.2.403-468.1976 VogelsGD Van der DriftC Degradation of purines and pyrimi-dines by microorganisms Bacteriol Rev 1976 Jun 40 2 403 468 https://doi.org/10.1128/br.40.2.403-468.1976 Search in Google Scholar

Waterborg JH. The Lowry method for protein quantitation. In: Walker JM, editor. The protein protocols handbook. Springer protocols handbooks. Totowa (USA): Humana Press; 2009. p. 7–10. https://doi.org/10.1007/978-1-59745-198-7_2 WaterborgJH The Lowry method for protein quantitation In: WalkerJM editor. The protein protocols handbook. Springer protocols handbooks Totowa (USA) Humana Press 2009 7 10 https://doi.org/10.1007/978-1-59745-198-7_2 Search in Google Scholar