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Polish Journal of Microbiology
Édition 72 (2023): Edition 2 (June 2023)
Accès libre
Mechanisms of Rapid Bactericidal and Anti-Biofilm Alpha-Mangostin
In Vitro
Activity against
Staphylococcus aureus
Xiangbin Deng
Xiangbin Deng
,
Hongbo Xu
Hongbo Xu
,
Duoyun Li
Duoyun Li
,
Jinlian Chen
Jinlian Chen
,
Zhijian Yu
Zhijian Yu
,
Qiwen Deng
Qiwen Deng
,
Peiyu Li
Peiyu Li
,
Jinxin Zheng
Jinxin Zheng
et
Haigang Zhang
Haigang Zhang
| 14 juin 2023
Polish Journal of Microbiology
Édition 72 (2023): Edition 2 (June 2023)
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Article Category:
ORIGINAL PAPER
Publié en ligne:
14 juin 2023
Pages:
199 - 208
Reçu:
27 janv. 2023
Accepté:
16 avr. 2023
DOI:
https://doi.org/10.33073/pjm-2023-021
Mots clés
alpha-mangostin
,
biofilm
,
cell membrane
,
SarT
© 2023 Xiangbin Deng et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Fig. 1.
The effect of sub-MIC of α-mangostin on the growth of Staphylococcus aureus planktonic cells. S. aureus SA113 was treated with α-mangostin, daptomycin, vancomycin, linezolid, and their synergistic combinations (½ × MIC), and the growth of planktonic cells was detected by optical density at 600 nm (OD600). The data presented was the average of three independent experiments (mean ± SD). MIC, minimum inhibitory concentration.
Fig. 2.
The antibacterial effect of α-mangostin on the planktonic cells of Staphylococcus aureus. S. aureus SA113 during logarithmic growth phase was treated with α-mangostin, daptomycin, vancomycin, linezolid, and their synergistic combinations (4 × MIC), the remaining planktonic cells were enumerated. The data presented was the average of three independent experiments (mean ± SD). MIC, minimum inhibitory concentration.
Fig. 3.
Effect of different concentrations of α-mangostin on Staphylococcus aureus biofilms. A) S. aureus SA113 and B) YUSA145 formed mature biofilms, then were treated with different concentrations of α-mangostin for 24 h. The remaining biofilm biomass was determined by crystal violet staining. The data presented was the average of three independent experiments (mean ± SD). Compared with control: *p < 0.05; **p < 0.01; ***p < 0.001; Student’s t-test. MIC – minimum inhibitory concentration
Fig. 4.
Schematic illustration of the SNPs in the sarT gene of the α-mangostin non-sensitive Staphylococcus aureus isolate. There were 35 SNPs located on both sides of the sarT gene, 10 SNPs in the sarT gene included one non-synonymous mutation (in red) and nine synonymous mutations.
Fig. 5.
The different abundance proteins in-α-mangostin-treated Staphylococcus aureus isolate. A) The molecular functions of different abundance proteins were classified by the GO analysis; B) different abundance proteins related to cell membrane synthesis and transport of biological process.
Fig. 6.
Protein-protein interaction network of different abundance proteins in α-mangostin-treated Staphylococcus aureus isolate. The protein-protein interaction network of different abundance proteins was analyzed through STRING database.
Fig. 7.
The fluorescence intensity was significantly increased in α-mangostin-treated Staphylococcus aureus isolate. S. aureus SA113 was treated with α-mangostin, and staining with A) propidium iodide or B) bis(1,3-dibutylbarbituric acid) trimethine oxonol to evaluate the integrity of S. aureus cell membrane. The results were expressed in a relative fluorescence units. The data presented was the average of three independent experiments (mean ± SD).
Staphylococcus aureus susceptibility to α-mangostin.
S. aureus
The MICs (μM) of α-mangostin
1.56
3.13
6.25
MIC
50
/MIC
90
MSSA (n = 190)
16
163
11
3.13/3.13
MRSA (n = 138)
13
117
8
3.13/3.13
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