Strains | Adhesion capacity to HT-29 Cell (%) |
---|---|
WDS-2 | 3.79 ± 0.72 |
WDS-3 | 9.65 ± 1.50 |
WDS-4 | 11.50 ± 1.22 |
WDS-7 | 10.74 ± 0.99 |
WDS-8 | 2.47 ± 0.46 |
WDS-9 | 9.43 ± 0.93 |
WDS-10 | 8.99 ± 1.05 |
WDS-11 | 3.32 ± 0.50 |
WDS-15 | 2.92 ± 0.80 |
WDS-18 | 11.23 ± 0.71 |
WDS-20 | 4.16 ± 0.37 |
LGG | 15.56 ± 1.31 |
Strains | Temperature (°C) | |||
---|---|---|---|---|
50 | 60 | 70 | 80 | |
WDS-7 | 91.81 ± 7.43% | 12.38 ± 2.33% |
≈ 0% | 0% |
LGG | 83.38 ± 7.32% | 4.79 ± 0.93% | ≈ 0% | 0% |
Strains | Antioxidant capacities (%) | ||
---|---|---|---|
DPPH scavenging activity | ABTS+ scavenging activity | Superoxide anion scavenging activity | |
WDS-3 | 19.32 ± 1.24 |
52.35 ± 2.47 |
41.31 ± 2.64 |
WDS-4 | 31.04 ± 1.71 |
65.20 ± 2.38 |
49.72 ± 1.63 |
WDS-7 | 30.15 ± 2.24 |
68.31 ± 1.50 |
48.92 ± 1.36 |
WDS-9 | 18.09 ± 1.78 |
40.78 ± 1.43 |
40.77 ± 1.85 |
WDS-10 | 22.08 ± 1.61 |
49.01 ± 1.72 |
41.94 ± 1.69 |
WDS-18 | 32.00 ± 2.71 |
56.97 ± 1.68 |
48.68 ± 1.33 |
LGG | 21.32 ± 1.13 |
51.94 ± 2.04 |
40.12 ± 1.07 |
Strains | Antibiotic susceptibility | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Metronidazole | Chloramphenicol | Streptomycin | Kanamycin | Ampicillin | Gentamicin | Tetracycline | Erythromycin | Rifampicin | Ciprofloxacin | Doxycycline | Vancomycin | |
WDS-7 | R | S | R | R | S | R | I | S | S | I | S | R |
LGG | R | S | R | R | S | R | S | S | S | S | S | R |
Enzyme | Strains | |
---|---|---|
LGG | WDS-7 | |
Alkaline phosphatase | 1 | 1 |
Esterase | 2 | 1 |
Esterase lipase | 1 | 2 |
Lipase | 0 | 0 |
Leucine arylamidase | 2 | 4 |
Valine arylamidase | 3 | 2 |
Cystine arylamidase | 1 | 0 |
Trypsin | 0 | 0 |
α-Chymotrypsin | 0 | 0 |
Acid phosphatase | 2 | 0 |
Naphthol-AS-BI-phosphohydrolase | 2 | 2 |
α-Galactosidase | 1 | 3 |
β-Galactosidase | 2 | 4 |
β-Glucuronidase | 0 | 0 |
α-Glucosidase | 2 | 2 |
β-Glucosidase | 1 | 0 |
N-Acetyl-b-glucosaminidase | 0 | 0 |
α-Mannosidase | 0 | 0 |
α-Fucosidase | 1 | 0 |
Control | 0 | 0 |
Characteristic | WDS | WDS-7 |
---|---|---|
Glucose gas production | − | − |
Gelatin | 3 | − |
Nitrate reduction | − | − |
Catalase | − | − |
Arginine hydrolysis | − | − |
Motile | − | − |
H2S production | − | − |
Indole | − | − |
15°C growth test | − | − |
45°C growth test | + | + |
Arabinose | 3 | − |
Cellobiose | 7 | − |
Esculin | 5 | − |
Fructose | + | + |
Gluconate | 4 | − |
Lactose | 9 | + |
Mannose | 6 | + |
Mannitol | 1 | − |
Sorbitol | − | − |
Melezitose | − | − |
Melibiose | 5 | − |
Raffinose | 3 | − |
Rhamnose | − | − |
Salicin | 6 | − |
Glucose | + | + |
Sucrose | 8 | + |
Trehalose | 6 | − |
Xylose | − | − |
Ribose | − | − |
Maltose | 8 | − |
Strains | Artificial simulated gastric juice, 3 h, survival rate (%) | Artificial simulated intestinal juice, 5 h survival rate (%) |
---|---|---|
WDS-3 | 81.34 ± 4.40 |
70.56 ± 4.49 |
WDS-4 | 77.45 ± 3.66 |
62.36 ± 3.06 |
WDS-7 | 78.99 ± 3.98 |
67.81 ± 3.57 |
WDS-9 | 76.95 ± 2.03 |
66.19 ± 3.48 |
WDS-10 | 70.94 ± 4.27 |
60.04 ± 1.25 |
WDS-18 | 71.29 ± 2.13 |
60.75 ± 5.17 |
LGG | 84.72 ± 4.96 |
77.75 ± 4.25 |
Strains | Biofilm formation | |
---|---|---|
Glass | Plastic | |
WDS-7 | + | ++ |
LGG | + | + |